FastQCFastQC Report
Thu 26 May 2016
SRR936105_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936105_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199515
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65110.20349959290704997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA1402.8731301E-722.81898710
GCGTAAG4600.020.06244338
TTGCGTA4900.019.55760436
TGCGTAA4850.019.02859537
ATTCGCG3051.8189894E-1218.62299556
CGCGGAA2901.4551915E-1118.36182859
CGATCGT3101.8189894E-1218.3177571
TCGCGCT3350.018.01271637
CGTAAGT5000.017.748139
CTCGCGC3551.8189894E-1216.99685336
CCTCGCG3800.016.81267535
CGGTTAC3452.0008883E-1015.4314434
AATTCGC4007.2759576E-1215.08682955
CGAACTC8750.014.6043825
TCGAACT9650.014.34673624
CGCGCTT4001.2187229E-1014.19803838
GGGCGTA3851.036824E-913.828608534
TCGCGGA3901.2551027E-913.65323858
TTAGTCG2901.4248017E-613.46344515
CGCTCCG4253.1832315E-1013.36348525
Produced by FastQC (version 0.11.5)