##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936105_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3199515 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.475982766137996 39.0 36.0 41.0 30.0 41.0 2 36.33774743984635 39.0 36.0 41.0 29.0 41.0 3 36.24780662069095 39.0 36.0 41.0 29.0 41.0 4 36.12602066250666 39.0 36.0 41.0 29.0 41.0 5 36.05809474248441 39.0 35.0 41.0 29.0 41.0 6 35.959589500283634 39.0 35.0 40.0 28.0 41.0 7 35.801793709359075 39.0 35.0 40.0 27.0 41.0 8 35.62718787066165 39.0 35.0 40.0 27.0 41.0 9 35.5416120880821 38.0 35.0 40.0 27.0 41.0 10-11 35.75600989524975 39.0 35.0 40.0 27.5 41.0 12-13 35.944701931386476 39.0 35.0 41.0 28.5 41.0 14-15 35.89151746436569 39.0 35.0 41.0 28.0 41.0 16-17 35.73426863133943 39.0 35.0 41.0 27.0 41.0 18-19 35.59824317123064 39.0 35.0 41.0 27.0 41.0 20-21 35.40715842869935 39.0 35.0 41.0 26.5 41.0 22-23 35.16658837355037 39.0 35.0 41.0 25.5 41.0 24-25 34.95965216603142 39.0 34.0 41.0 24.5 41.0 26-27 34.03203782448277 38.0 33.0 40.0 23.5 40.5 28-29 34.06905687268227 38.0 33.5 39.5 23.0 40.5 30-31 34.45081270130004 38.0 34.0 40.0 23.0 41.0 32-33 34.303517564380854 38.0 34.0 40.0 23.0 41.0 34-35 34.03723767508513 38.0 33.0 40.0 22.0 41.0 36-37 33.75135856528255 38.0 33.0 40.0 21.5 41.0 38-39 33.47520014752236 37.0 33.0 40.0 20.5 41.0 40-41 33.097042207959646 37.0 32.0 40.0 18.5 41.0 42-43 32.74130047835375 36.0 32.0 39.0 18.0 41.0 44-45 32.378147625499494 36.0 32.0 39.0 15.5 41.0 46-47 31.988763140663508 35.0 31.0 39.0 12.5 40.0 48-49 31.520264008763828 35.0 31.0 38.0 8.0 40.0 50-51 31.074355800801058 35.0 31.0 37.0 7.0 39.0 52-53 30.615999612441257 35.0 30.0 36.5 2.0 39.0 54-55 30.151453892230542 34.5 30.0 36.0 2.0 38.5 56-57 29.68464454768926 34.0 29.5 36.0 2.0 37.0 58-59 29.249082126509798 34.0 29.0 35.0 2.0 37.0 60-61 28.889924097871084 34.0 29.0 35.0 2.0 36.5 62-63 28.538000915763796 34.0 29.0 35.0 2.0 36.0 64-65 28.163711375005274 34.0 28.5 35.0 2.0 36.0 66-67 27.78180349209177 34.0 27.0 35.0 2.0 35.0 68-69 27.417458896113942 34.0 26.5 35.0 2.0 35.0 70-71 26.99288220245881 33.0 26.0 35.0 2.0 35.0 72-73 26.503190796105034 33.0 25.0 35.0 2.0 35.0 74-75 25.915862091598257 33.0 24.5 35.0 2.0 35.0 76-77 24.202006397844674 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6507371214786559 1101 2 0.6577934998181476 1101 3 0.718197254218083 1101 4 0.7219734845076502 1101 5 0.8342768584850404 1101 6 0.6708097765196328 1101 7 0.7812739515749456 1101 8 1.0471473584748665 1101 9 1.076571385813594 1101 10-11 0.842141422209373 1101 12-13 0.9342145184462396 1101 14-15 0.9589432974622412 1101 16-17 1.071120435402804 1101 18-19 0.9331527747266577 1101 20-21 1.0151823786078609 1101 22-23 0.9959535617039634 1101 24-25 1.0871370699576843 1101 26-27 0.9233035866140327 1101 28-29 1.01798924152164 1101 30-31 1.0463202145328552 1101 32-33 1.0856436980810642 1101 34-35 1.0869680563923367 1101 36-37 1.0878986930050658 1101 38-39 1.0815254820186126 1101 40-41 1.0681824793137835 1101 42-43 1.0858184177401498 1101 44-45 0.9038878868902529 1101 46-47 0.9011470365686947 1101 48-49 1.0500296858960922 1101 50-51 0.9229680458977434 1101 52-53 0.9802146982740645 1101 54-55 0.9916148104236413 1101 56-57 0.9295311875488785 1101 58-59 1.0704843499976668 1101 60-61 1.0081059873779274 1101 62-63 0.777588135675952 1101 64-65 0.7617620986171296 1101 66-67 0.8095247509486896 1101 68-69 0.7434129700971717 1101 70-71 0.8024232133909486 1101 72-73 0.6974634449726231 1101 74-75 0.6130593079943907 1101 76-77 0.5478420521714114 1102 1 0.7208574227531628 1102 2 1.0042517369551476 1102 3 0.9049819879218006 1102 4 0.9194800755081403 1102 5 0.9109683003625904 1102 6 0.9425997979186604 1102 7 0.8338232165108224 1102 8 1.0414550179373592 1102 9 0.9150707726395382 1102 10-11 0.786827909044753 1102 12-13 0.8242254910346247 1102 14-15 0.8566226694925732 1102 16-17 0.9083549955807442 1102 18-19 0.9736371574068485 1102 20-21 0.9876416782488988 1102 22-23 1.0738514918070834 1102 24-25 1.0269591253568464 1102 26-27 0.9127058536203023 1102 28-29 0.8816759319208742 1102 30-31 0.9640456615044783 1102 32-33 0.9762181950052664 1102 34-35 1.0390573166611432 1102 36-37 0.9506395562151155 1102 38-39 1.0548995777730639 1102 40-41 1.178691587555143 1102 42-43 1.0332244680551739 1102 44-45 0.9960700417874548 1102 46-47 0.8892373096918078 1102 48-49 0.8563150923535261 1102 50-51 0.9800155755758588 1102 52-53 0.847972665813078 1102 54-55 0.7807003122085483 1102 56-57 0.9240386871392694 1102 58-59 1.0053898417533276 1102 60-61 0.9100465467316887 1102 62-63 0.8168076917081422 1102 64-65 0.8094494146028488 1102 66-67 0.8525797479448833 1102 68-69 0.7069055300864449 1102 70-71 0.8978450125975463 1102 72-73 0.9346463694405003 1102 74-75 0.9817643439656152 1102 76-77 1.14000062345103 1103 1 0.37377082815815044 1103 2 0.25226337570007473 1103 3 0.25998501040552213 1103 4 0.4227562662637041 1103 5 0.4965230981146078 1103 6 0.4152069459267693 1103 7 0.469001225261529 1103 8 0.5142489976945726 1103 9 0.48847836373573017 1103 10-11 0.42037052885890347 1103 12-13 0.39859151154281847 1103 14-15 0.4616563358753183 1103 16-17 0.574096757784794 1103 18-19 0.654239551856513 1103 20-21 0.5739237229495231 1103 22-23 0.5311829617119841 1103 24-25 0.41858256753801015 1103 26-27 0.3035996043252922 1103 28-29 0.34091151418463994 1103 30-31 0.6059827889541083 1103 32-33 0.6712368415122612 1103 34-35 0.6292100961413638 1103 36-37 0.6599635779937572 1103 38-39 0.8322261656072101 1103 40-41 0.7934939352794075 1103 42-43 0.7317178358772978 1103 44-45 0.672743556623459 1103 46-47 0.4824310814546564 1103 48-49 0.4983189204836833 1103 50-51 0.5384879763358974 1103 52-53 0.5099842535721386 1103 54-55 0.5428069222459868 1103 56-57 0.5628990274384584 1103 58-59 0.5714715329752735 1103 60-61 0.6293121128720465 1103 62-63 0.5712985507465582 1103 64-65 0.6025702696008075 1103 66-67 0.5840581737669162 1103 68-69 0.46757790088355833 1103 70-71 0.39297918049258485 1103 72-73 0.5141011978887988 1103 74-75 0.5156247174405344 1103 76-77 0.5740380156430014 1104 1 0.6207479129974516 1104 2 0.6644639597832764 1104 3 0.5399942532206126 1104 4 0.5535240367856034 1104 5 0.627405381389849 1104 6 0.44264945012983503 1104 7 0.49112399861046185 1104 8 0.6202908745994975 1104 9 0.6340997334514071 1104 10-11 0.5601638469715837 1104 12-13 0.7095740684558223 1104 14-15 0.7041125526397849 1104 16-17 0.684628488893857 1104 18-19 0.7466356664660552 1104 20-21 0.6614244173534942 1104 22-23 0.6272716013993076 1104 24-25 0.6803002724706815 1104 26-27 0.5436657081754035 1104 28-29 0.528981500562395 1104 30-31 0.7186972490179571 1104 32-33 0.7240682886228811 1104 34-35 0.84362397999476 1104 36-37 0.8570660986035037 1104 38-39 0.883724868307219 1104 40-41 0.7716671569491709 1104 42-43 0.8354638343211533 1104 44-45 0.8671333859824202 1104 46-47 0.6837233715086413 1104 48-49 0.9006988431262357 1104 50-51 0.7223267846398436 1104 52-53 0.6429911573960823 1104 54-55 0.6249461363545059 1104 56-57 0.6794709654747884 1104 58-59 0.638266890164747 1104 60-61 0.43107485948769764 1104 62-63 0.5600038784183248 1104 64-65 0.49215219921567765 1104 66-67 0.549578417815404 1104 68-69 0.5731630900221596 1104 70-71 0.5165476432713412 1104 72-73 0.3831608062074672 1104 74-75 0.3662355032468163 1104 76-77 0.49226278850047933 1105 1 0.44736287812224873 1105 2 0.5389765334628436 1105 3 0.5310120894338652 1105 4 0.6785883981910175 1105 5 0.605288291094034 1105 6 0.4006012893226796 1105 7 0.46643234146385737 1105 8 0.6883440303163724 1105 9 0.6115668800931857 1105 10-11 0.674693847739384 1105 12-13 0.6957854048696532 1105 14-15 0.3695107990003095 1105 16-17 0.40583368843245893 1105 18-19 0.6957834075051963 1105 20-21 0.6536822409013539 1105 22-23 0.6306975532013226 1105 24-25 0.5744638463602243 1105 26-27 0.4707349149132014 1105 28-29 0.6344144993159873 1105 30-31 0.6328874813075913 1105 32-33 0.7151508458024765 1105 34-35 0.7285003164827089 1105 36-37 0.7302788872009245 1105 38-39 0.6603930595122094 1105 40-41 0.6637583323062657 1105 42-43 0.601399463843741 1105 44-45 0.4835006324383144 1105 46-47 0.4176089019141074 1105 48-49 0.3920113533460565 1105 50-51 0.4685259490866365 1105 52-53 0.45561261984210333 1105 54-55 0.3709635842419594 1105 56-57 0.40540582940919023 1105 58-59 0.44114950668848607 1105 60-61 0.09975606303657614 1105 62-63 0.17752437505050978 1105 64-65 0.335431540880645 1105 66-67 0.4846849175484458 1105 68-69 0.11404284377713836 1105 70-71 -0.08509226431818107 1105 72-73 -0.06558715840111518 1105 74-75 -0.13241830065330262 1105 76-77 0.12384576819181348 1106 1 0.2049707357245154 1106 2 0.19107531632103303 1106 3 0.02902842481655199 1106 4 0.14151974759341357 1106 5 -0.008630829186884625 1106 6 0.36712778116586264 1106 7 0.39145678772226233 1106 8 0.3149328151764266 1106 9 0.3033718018363061 1106 10-11 0.05396550681361845 1106 12-13 0.08437149897676477 1106 14-15 0.12822711631740447 1106 16-17 0.3337128983933084 1106 18-19 0.3260755286414465 1106 20-21 0.11391973025396851 1106 22-23 0.2317135321546857 1106 24-25 0.3089218851289033 1106 26-27 0.17959495803093972 1106 28-29 0.1670186838762575 1106 30-31 0.1903048955659159 1106 32-33 0.111246590799702 1106 34-35 0.11265357044283064 1106 36-37 0.08483441360888122 1106 38-39 0.16690976841331207 1106 40-41 0.2000752854258181 1106 42-43 0.20735196686096202 1106 44-45 -0.017199021030705808 1106 46-47 0.1653797760004565 1106 48-49 0.029804469865606364 1106 50-51 -0.03726436304869196 1106 52-53 -0.03925477557385548 1106 54-55 0.13375509172497502 1106 56-57 0.1848529733360671 1106 58-59 -0.05394337577727626 1106 60-61 -0.17161970980031427 1106 62-63 -0.18269773413227242 1106 64-65 -0.05822545958231018 1106 66-67 -0.03698425477648115 1106 68-69 -0.237056805075607 1106 70-71 -0.2937723172810074 1106 72-73 -0.21858356170512394 1106 74-75 -0.3193685927433698 1106 76-77 -0.3010702622950987 1107 1 0.2514272526035697 1107 2 0.2535836999561738 1107 3 0.162545080305037 1107 4 0.1788816970680358 1107 5 0.30041902549677246 1107 6 -0.03767883631680746 1107 7 0.20784399988411906 1107 8 0.30543583328508106 1107 9 0.13687504350856017 1107 10-11 0.26483293359653715 1107 12-13 0.3295492320418205 1107 14-15 0.2424077557093085 1107 16-17 0.20069255410535902 1107 18-19 0.27905339584467015 1107 20-21 0.20035836204471735 1107 22-23 0.29932140159353793 1107 24-25 0.14414880218681247 1107 26-27 0.040853621120596983 1107 28-29 0.23202650860238805 1107 30-31 0.3555541689842059 1107 32-33 0.43470511975117887 1107 34-35 0.4894387258195323 1107 36-37 0.4399897903135539 1107 38-39 0.4424295537922518 1107 40-41 0.4883136042275211 1107 42-43 0.553830840100396 1107 44-45 0.46450900490268054 1107 46-47 0.3702981752850505 1107 48-49 0.5708440406234843 1107 50-51 0.5435270521089208 1107 52-53 0.60761980524439 1107 54-55 0.5873567013829231 1107 56-57 0.5284545829940122 1107 58-59 0.6544111960984225 1107 60-61 0.5633992931060128 1107 62-63 0.6608524558967908 1107 64-65 0.458394258727548 1107 66-67 0.6186137778638034 1107 68-69 0.5201486498763295 1107 70-71 0.47033901739370876 1107 72-73 0.5373185174363897 1107 74-75 0.6067101016451879 1107 76-77 0.6021691743246187 1108 1 0.09857572851310437 1108 2 0.23910835229498417 1108 3 0.15525332584514473 1108 4 0.18055187546387685 1108 5 0.11891319602634098 1108 6 0.2027833072426759 1108 7 -0.01667489321884119 1108 8 0.10336250837989525 1108 9 0.050973933802140436 1108 10-11 0.05779823771228365 1108 12-13 0.17049739891919558 1108 14-15 0.29316067044452154 1108 16-17 0.20225527358842754 1108 18-19 0.2058148791990746 1108 20-21 0.2027751340632591 1108 22-23 0.18775657507296017 1108 24-25 0.12158102581372532 1108 26-27 0.029548662558994465 1108 28-29 0.2599672306139027 1108 30-31 0.24864238362108892 1108 32-33 0.20214225979894707 1108 34-35 0.37886069519724686 1108 36-37 0.3486384769268369 1108 38-39 0.27074813786304475 1108 40-41 0.3084786554987673 1108 42-43 0.3820335371084056 1108 44-45 0.31497395784550264 1108 46-47 0.23173587720947353 1108 48-49 0.35700291125138506 1108 50-51 0.3311355645404426 1108 52-53 0.348782889947298 1108 54-55 0.28733141693286157 1108 56-57 0.23834501704373778 1108 58-59 0.2629187111988571 1108 60-61 0.29003294951843017 1108 62-63 0.22789487758523208 1108 64-65 0.2008763489087535 1108 66-67 0.09594978011131161 1108 68-69 0.12984593882140416 1108 70-71 0.13442667015392473 1108 72-73 0.1962839620213046 1108 74-75 0.11806352206940218 1108 76-77 0.14291295856397568 1109 1 -0.05924886945884822 1109 2 -0.0057371657332936365 1109 3 -0.33254025821548794 1109 4 -0.040864252989912586 1109 5 -0.18093321862806988 1109 6 -0.15424620090604435 1109 7 -0.19546692028109192 1109 8 -0.1638064586783443 1109 9 -0.07583837937533389 1109 10-11 -0.26165999272591733 1109 12-13 -0.14619308289637445 1109 14-15 0.008113974725276307 1109 16-17 0.05215553663942529 1109 18-19 -0.12420458023093062 1109 20-21 -0.11310975662765088 1109 22-23 -0.07363647288250519 1109 24-25 -0.08918111045799293 1109 26-27 -0.1776359151463609 1109 28-29 -0.1348250794650241 1109 30-31 -0.043942121866216155 1109 32-33 -0.021643167607074076 1109 34-35 0.06487925434143449 1109 36-37 0.07874496601951364 1109 38-39 0.04798025911780002 1109 40-41 0.13993352999347053 1109 42-43 0.12616078848373746 1109 44-45 -0.022457763363547656 1109 46-47 -0.14068708512125028 1109 48-49 -0.03235113773734 1109 50-51 -0.027596567490096646 1109 52-53 0.07051232925302742 1109 54-55 0.10909037780621134 1109 56-57 0.12976582991156604 1109 58-59 0.05023142104698408 1109 60-61 -0.02817412150696086 1109 62-63 -0.11676347736404225 1109 64-65 0.014102417835513137 1109 66-67 -0.07399375684086706 1109 68-69 -0.12901210716799127 1109 70-71 -0.11539380130469468 1109 72-73 -0.01639956677381349 1109 74-75 -0.024793163325430356 1109 76-77 0.2221102057387938 1110 1 -0.536537384728156 1110 2 -0.5371335996218818 1110 3 -0.6183028200365328 1110 4 -0.49248257860003974 1110 5 -0.29192977900392947 1110 6 -0.6082434334636844 1110 7 -0.7615753290288794 1110 8 -0.6654261317541028 1110 9 -0.6149312655129364 1110 10-11 -0.5397762687341796 1110 12-13 -0.4962132754928348 1110 14-15 -0.6566318263682405 1110 16-17 -0.7154215708695304 1110 18-19 -0.5737856638298311 1110 20-21 -0.7052293413910888 1110 22-23 -0.9458242684888418 1110 24-25 -0.8515875851951407 1110 26-27 -0.812590220551094 1110 28-29 -0.6679574174574867 1110 30-31 -0.6521994978833874 1110 32-33 -0.504549451275544 1110 34-35 -0.32925526654534565 1110 36-37 -0.3874444069940637 1110 38-39 -0.32758250166283887 1110 40-41 -0.30477661076142937 1110 42-43 -0.36563871987549845 1110 44-45 -0.38918197359843276 1110 46-47 -0.3906823350625501 1110 48-49 -0.27156705039519835 1110 50-51 -0.311379518114105 1110 52-53 -0.3391915268834005 1110 54-55 -0.458713220256616 1110 56-57 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-0.6464102646899619 2314 14-15 -0.689216866986456 2314 16-17 -1.0840120885733668 2314 18-19 -0.8466035858054326 2314 20-21 -0.6442680280511297 2314 22-23 -0.4912364839074783 2314 24-25 -0.39138043128212274 2314 26-27 -0.585618379651855 2314 28-29 -0.6632732147428229 2314 30-31 -0.8770405506456882 2314 32-33 -0.8906250817995911 2314 34-35 -0.8705618199334637 2314 36-37 -0.8832889257395635 2314 38-39 -0.9172485359043563 2314 40-41 -0.6513338393395216 2314 42-43 -0.6296725162805075 2314 44-45 -0.5532551710312958 2314 46-47 -0.36585271282299203 2314 48-49 -0.38943467123152686 2314 50-51 -0.41929847905567286 2314 52-53 -0.48308295668556056 2314 54-55 -0.4489749569085326 2314 56-57 -0.3297954390460127 2314 58-59 -0.4653035849916556 2314 60-61 -0.4398789611561078 2314 62-63 -0.4063893504616516 2314 64-65 -0.4395330973510916 2314 66-67 -0.3900726786459785 2314 68-69 -0.2791885960861471 2314 70-71 -0.22067482374179193 2314 72-73 -0.10143036481949963 2314 74-75 -0.05662684205409718 2314 76-77 -0.04230671388837948 2315 1 -1.4452832930865682 2315 2 -1.4332967665161291 2315 3 -1.3797725567064774 2315 4 -1.3413521280637468 2315 5 -1.3086871970788394 2315 6 -1.359282629349174 2315 7 -1.4149524780143992 2315 8 -1.3692315401578057 2315 9 -1.2568976690396667 2315 10-11 -1.4406590120994878 2315 12-13 -1.5017998270623423 2315 14-15 -1.8588620855605313 2315 16-17 -1.6615449816015655 2315 18-19 -1.5573729669082255 2315 20-21 -1.5547366110094458 2315 22-23 -1.5139848386514743 2315 24-25 -1.6448116477940502 2315 26-27 -1.5394446973409899 2315 28-29 -1.5768794607117442 2315 30-31 -1.6809461107813775 2315 32-33 -1.6919541846632242 2315 34-35 -1.6880501571795605 2315 36-37 -1.7480786491834621 2315 38-39 -1.54906262749585 2315 40-41 -1.6207908514075484 2315 42-43 -1.7273026522575847 2315 44-45 -1.6044641081520723 2315 46-47 -1.7000157511513265 2315 48-49 -1.5146060874797058 2315 50-51 -1.429252345364354 2315 52-53 -1.5244952225849246 2315 54-55 -1.6018057687999523 2315 56-57 -1.6458355486430563 2315 58-59 -1.5528797895599098 2315 60-61 -1.5640680921164751 2315 62-63 -1.5614669480552656 2315 64-65 -1.6026332171978872 2315 66-67 -1.5569217523656036 2315 68-69 -1.4207050768957679 2315 70-71 -1.640722913872306 2315 72-73 -3.03297921204501 2315 74-75 -3.5170537762003242 2315 76-77 -3.149979425375001 2316 1 -3.312159021234507 2316 2 -3.1222898868886304 2316 3 -2.725542164185086 2316 4 -2.7496703184623996 2316 5 -3.393588054553476 2316 6 -2.7499318910159047 2316 7 -3.2289123093542003 2316 8 -3.08050455725963 2316 9 -2.7636291245855347 2316 10-11 -2.769612652791494 2316 12-13 -2.8024446014628097 2316 14-15 -2.926243894399178 2316 16-17 -4.2398335964028036 2316 18-19 -3.8088011776394026 2316 20-21 -3.300277986019694 2316 22-23 -3.4455472748938405 2316 24-25 -3.522608363509203 2316 26-27 -3.2076050405215213 2316 28-29 -3.3706579152870724 2316 30-31 -3.6114296725140207 2316 32-33 -3.7005974952996326 2316 34-35 -3.7111139748770583 2316 36-37 -3.6313020333703605 2316 38-39 -3.6715906586092544 2316 40-41 -3.7288323932602054 2316 42-43 -3.8957109550349216 2316 44-45 -3.5589145419741115 2316 46-47 -3.1831761880814042 2316 48-49 -3.475369559924644 2316 50-51 -3.7352978879390406 2316 52-53 -4.838264059092712 2316 54-55 -3.938188862166239 2316 56-57 -3.8454243138884827 2316 58-59 -3.80336315584859 2316 60-61 -3.591002613939775 2316 62-63 -3.659104435732761 2316 64-65 -3.5766417124954764 2316 66-67 -3.7466408755563165 2316 68-69 -3.719631811468883 2316 70-71 -3.4746070257717605 2316 72-73 -3.4650410235022875 2316 74-75 -3.5099922507586037 2316 76-77 -3.5069675163228666 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82832.0 3 7035.0 4 8789.0 5 10370.0 6 11855.0 7 12573.0 8 14350.0 9 14874.0 10 17645.0 11 19601.0 12 16173.0 13 14174.0 14 14537.0 15 15137.0 16 15549.0 17 16692.0 18 17478.0 19 18632.0 20 19814.0 21 21398.0 22 23943.0 23 27375.0 24 31856.0 25 37207.0 26 43751.0 27 51938.0 28 61330.0 29 72203.0 30 85508.0 31 102732.0 32 125242.0 33 155935.0 34 205890.0 35 273308.0 36 386146.0 37 527854.0 38 487929.0 39 128885.0 40 975.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.63917454879627 26.79228061840074 28.403267046342545 22.16527778646045 2 22.41438540828208 26.97767323366911 28.318560833991075 22.289380524057737 3 22.60861709646493 27.214283950374156 27.89188945874396 22.285209494416954 4 22.179038804432363 27.222058282921996 28.200390370038786 22.398512542606852 5 22.463431292716894 27.179985714799248 28.169415604002985 22.187167388480876 6 22.66859047975089 26.94640726031054 28.211285189244066 22.173717070694504 7 22.57752825496758 26.78234883142987 28.257937258420224 22.38218565518233 8 22.42648541399715 27.111374672038057 28.04128139376909 22.4208585201957 9 22.419721239520378 26.915919519766973 28.120253320858396 22.544105919854253 10-11 22.776497634626825 27.002736286446954 27.9355683163476 22.28519776257862 12-13 22.8251291113457 26.79995486065415 27.954978191506484 22.419937836493663 14-15 22.559338218861015 27.0708557800356 28.025049660154195 22.344756340949193 16-17 22.75791709032533 26.92510860925455 28.009935257975698 22.307039042444423 18-19 22.64647715370756 26.83809663438477 27.878874175922164 22.636552035985506 20-21 22.65652122466212 27.00611974670679 27.794116658619235 22.54324237001185 22-23 22.726608423651275 27.043253355866515 27.495042341149766 22.73509587933245 24-25 22.716259679329543 27.149248419335258 27.457105062800665 22.677386838534535 26-27 22.752370873929063 27.131754470154423 27.380979549600386 22.73489510631613 28-29 22.84603659418087 27.1307155843549 27.38885626813623 22.634391553328 30-31 22.688173101733746 26.98103620919856 27.4681067638651 22.862683925202592 32-33 22.617416423725174 27.281533565779874 27.41497490668249 22.686075103812456 34-35 22.778408852917952 27.038134790564673 27.422896128505098 22.760560228012274 36-37 22.834355483036067 27.043986821458265 27.630238126496497 22.49141956900917 38-39 22.70578969636639 26.987422619279272 27.664079481431365 22.64270820292297 40-41 22.615600251068415 26.972240560552752 27.57889024759247 22.833268940786365 42-43 22.54865506656795 27.156606646807486 27.387098785895514 22.90763950072905 44-45 22.5455826505066 26.976451659993394 27.549882325506807 22.9280833639932 46-47 22.48173979891465 26.98320547636432 27.607820260253295 22.927234464467734 48-49 22.618605477076102 26.8979502387522 27.47654213537065 23.00690214880105 50-51 22.428507867800565 27.056732521670178 27.371557104439283 23.143202506089974 52-53 22.420654773220736 26.976407802447394 27.492014198092914 23.110923226238956 54-55 22.485432237020948 26.885547725534387 27.509166939834206 23.11985309761046 56-57 22.35310888518857 27.081925589204907 27.466551374278698 23.098414151327823 58-59 22.39921024357596 27.082324542249815 27.43079706192104 23.087668152253187 60-61 22.42803435518995 26.848745380324857 27.339160902558383 23.384059361926813 62-63 22.376169618364308 26.76217498095675 27.53676992984048 23.32488547083846 64-65 22.376604241001854 26.682665177696336 27.564604982569108 23.37612559873271 66-67 22.330914653610026 26.74350187599048 27.666818713446634 23.258764756952857 68-69 22.31867666964863 26.6247002529304 27.675992061568806 23.38063101585216 70-71 22.183416656021024 26.532921934566584 27.692940116336434 23.590721293075966 72-73 22.236721320603532 26.39441207563743 27.672475534140055 23.696391069618976 74-75 22.160412832363416 26.364860762297155 27.601837556352233 23.8728888489872 76-77 21.980470341058897 26.277961968032027 27.90277966347721 23.83878802743187 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6647.0 1 4060.0 2 1368.5 3 1297.5 4 1331.0 5 1375.0 6 1428.5 7 1553.5 8 1766.0 9 1863.0 10 1829.5 11 1946.0 12 2158.5 13 2221.0 14 2388.0 15 2677.5 16 3033.5 17 3267.0 18 3514.5 19 4249.0 20 5359.0 21 7010.0 22 8038.0 23 9344.0 24 12666.5 25 17284.5 26 19886.0 27 23654.5 28 32390.0 29 42134.0 30 46911.0 31 54028.5 32 67773.5 33 80391.0 34 90875.0 35 95369.0 36 102873.0 37 116235.5 38 125089.0 39 128084.0 40 131923.5 41 139429.0 42 148468.5 43 153842.0 44 154333.5 45 155705.0 46 158565.5 47 160142.5 48 159739.0 49 159218.0 50 156121.0 51 149230.5 52 144916.0 53 140265.0 54 129734.5 55 115386.0 56 98902.0 57 90887.0 58 84948.0 59 71167.5 60 57527.0 61 51728.0 62 45323.0 63 32945.0 64 23323.0 65 19674.0 66 17107.0 67 12856.5 68 9414.0 69 6594.0 70 5533.0 71 4599.5 72 3125.5 73 2336.0 74 2087.0 75 1806.0 76 1196.5 77 724.5 78 581.0 79 473.0 80 312.5 81 216.5 82 146.5 83 120.0 84 111.0 85 83.0 86 66.5 87 69.0 88 68.0 89 56.0 90 47.0 91 49.0 92 50.5 93 48.0 94 43.5 95 38.5 96 34.0 97 38.0 98 46.0 99 100.5 100 151.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009220147428594646 2 0.013752084300276761 3 0.014252160093014096 4 0.01559611378599569 5 0.012501894818433418 6 0.00978273269542415 7 0.016252463263963443 8 0.018502804331281462 9 0.017752690642175457 10-11 0.02044059802813864 12-13 0.016627520108516446 14-15 0.008438779002442557 16-17 0.006313456883308877 18-19 0.017518280114329828 20-21 0.024113029631053457 22-23 0.02114382961167552 24-25 0.019487328548233093 26-27 0.025003789636866836 28-29 0.00907950111188727 30-31 0.008954482163702936 32-33 0.011142313756928784 34-35 0.012158092710926499 36-37 0.011720526392281331 38-39 0.014955391676550977 40-41 0.012095583236834333 42-43 0.01533044852110398 44-45 0.013627065352092427 46-47 0.025738275957449805 48-49 0.02503504437391292 50-51 0.03831830761849843 52-53 0.04185009290470587 54-55 0.029020023347288572 56-57 0.021612650667366774 58-59 0.017315124323530287 60-61 0.021784551721120232 62-63 0.027363522283846142 64-65 0.027629187548737857 66-67 0.029316943349226365 68-69 0.04381914133860913 70-71 0.056461682473749934 72-73 0.08640372056389797 74-75 0.08655999424912839 76-77 0.09192018165253171 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3199515.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.385137577140554 #Duplication Level Percentage of deduplicated Percentage of total 1 73.57044515215848 40.01138781211612 2 13.722480476414606 14.925979772188672 3 5.245775596586275 8.558766825574535 4 2.5542134049318057 5.556449897143715 5 1.4320258391991536 3.8940461139433067 6 0.8522369151746214 2.7809413128053793 7 0.5596364959265705 2.130513547690878 8 0.3724191229730427 1.6203252191397561 9 0.27368659434171166 1.339603477766374 >10 1.3003824305519305 12.362849479673505 >50 0.07941826874432734 2.971243985091826 >100 0.036644600023412896 3.380564157187037 >500 5.196297062700563E-4 0.19684752674971953 >1k 5.7736633891791946E-5 0.0660345107696915 >5k 5.7736633891791946E-5 0.20444636215951562 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6511 0.20349959290704997 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.1254737046083544E-5 0.0 2 0.0 0.0 0.0 1.5627368523041772E-4 0.0 3 0.0 0.0 0.0 4.688210556912532E-4 0.0 4 0.0 0.0 0.0 7.813684261520887E-4 0.0 5 0.0 0.0 0.0 0.0019690484339032633 0.0 6 0.0 0.0 0.0 0.004844484242142949 0.0 7 0.0 3.1254737046083544E-5 0.0 0.01100166744022141 0.0 8 0.0 3.1254737046083544E-5 0.0 0.0169088127419312 0.0 9 0.0 3.1254737046083544E-5 0.0 0.02350356225865483 0.0 10 0.0 3.1254737046083544E-5 0.0 0.030817170727438378 0.0 11 0.0 3.1254737046083544E-5 0.0 0.0408499413192312 0.0 12 0.0 3.1254737046083544E-5 0.0 0.05497708246406096 0.0 13 0.0 3.1254737046083544E-5 0.0 0.07182338573189999 0.0 14 0.0 3.1254737046083544E-5 0.0 0.0927015500786838 0.0 15 0.0 3.1254737046083544E-5 0.0 0.11529872496300221 0.0 16 0.0 3.1254737046083544E-5 0.0 0.13789589984732062 0.0 17 0.0 3.1254737046083544E-5 0.0 0.16068060315391552 0.0 18 0.0 3.1254737046083544E-5 0.0 0.18359032540869474 0.0 19 0.0 6.250947409216709E-5 0.0 0.20996932347558928 0.0 20 0.0 6.250947409216709E-5 0.0 0.23984885209164514 0.0 21 0.0 6.250947409216709E-5 0.0 0.2708848059784061 0.0 22 0.0 6.250947409216709E-5 0.0 0.3054212904143284 0.0 23 0.0 6.250947409216709E-5 0.0 0.3428644653955365 0.0 24 0.0 9.376421113825065E-5 0.0 0.3791512151060395 0.0 25 0.0 9.376421113825065E-5 0.0 0.4175320321986301 3.1254737046083544E-5 26 0.0 9.376421113825065E-5 0.0 0.4572568029842023 3.1254737046083544E-5 27 0.0 9.376421113825065E-5 0.0 0.49773168745888047 3.1254737046083544E-5 28 0.0 1.2501894818433418E-4 0.0 0.5373939487703605 3.1254737046083544E-5 29 0.0 1.2501894818433418E-4 0.0 0.5781813806154995 3.1254737046083544E-5 30 0.0 1.2501894818433418E-4 0.0 0.6229069093284451 3.1254737046083544E-5 31 0.0 1.2501894818433418E-4 0.0 0.6702890906903077 3.1254737046083544E-5 32 0.0 1.2501894818433418E-4 0.0 0.7230783415611428 3.1254737046083544E-5 33 0.0 1.2501894818433418E-4 0.0 0.7794306324552315 3.1254737046083544E-5 34 0.0 2.1878315932258484E-4 0.0 0.8353141022936289 3.1254737046083544E-5 35 0.0 2.1878315932258484E-4 0.0 0.8968234248003213 3.1254737046083544E-5 36 0.0 2.1878315932258484E-4 0.0 0.960333050477963 3.1254737046083544E-5 37 0.0 2.1878315932258484E-4 0.0 1.0238426761556048 3.1254737046083544E-5 38 0.0 2.1878315932258484E-4 0.0 1.088227434470537 3.1254737046083544E-5 39 0.0 2.1878315932258484E-4 0.0 1.154800024378695 3.1254737046083544E-5 40 0.0 2.1878315932258484E-4 0.0 1.2258420416844429 6.250947409216709E-5 41 0.0 2.1878315932258484E-4 0.0 1.3050415453592186 6.250947409216709E-5 42 0.0 2.1878315932258484E-4 0.0 1.3860850785197132 6.250947409216709E-5 43 0.0 2.1878315932258484E-4 0.0 1.4800680728172864 6.250947409216709E-5 44 0.0 2.1878315932258484E-4 0.0 1.5801144861017997 6.250947409216709E-5 45 0.0 2.1878315932258484E-4 0.0 1.6806297204420044 6.250947409216709E-5 46 0.0 2.5003789636866836E-4 0.0 1.78895863904373 6.250947409216709E-5 47 0.0 2.812926334147519E-4 0.0 1.898193945019792 6.250947409216709E-5 48 0.0 2.812926334147519E-4 0.0 2.012898829978919 6.250947409216709E-5 49 0.0 2.812926334147519E-4 0.0 2.129666527583087 6.250947409216709E-5 50 0.0 2.812926334147519E-4 0.0 2.2500597746846007 6.250947409216709E-5 51 0.0 3.1254737046083544E-4 0.0 2.3782667060476355 6.250947409216709E-5 52 0.0 3.1254737046083544E-4 0.0 2.517694088010214 6.250947409216709E-5 53 0.0 3.1254737046083544E-4 0.0 2.6648413900231755 6.250947409216709E-5 54 0.0 3.1254737046083544E-4 0.0 2.8208337826201784 6.250947409216709E-5 55 0.0 3.1254737046083544E-4 0.0 2.9851711900084856 6.250947409216709E-5 56 0.0 3.1254737046083544E-4 0.0 3.158822509036526 6.250947409216709E-5 57 0.0 3.1254737046083544E-4 0.0 3.334411621761423 6.250947409216709E-5 58 0.0 3.1254737046083544E-4 0.0 3.513126208190929 6.250947409216709E-5 59 0.0 3.1254737046083544E-4 0.0 3.6977479399221442 6.250947409216709E-5 60 0.0 3.1254737046083544E-4 0.0 3.8878392506364245 6.250947409216709E-5 61 0.0 3.1254737046083544E-4 0.0 4.088119605627728 6.250947409216709E-5 62 0.0 3.1254737046083544E-4 0.0 4.297182541728981 6.250947409216709E-5 63 0.0 3.1254737046083544E-4 0.0 4.517403418955685 6.250947409216709E-5 64 0.0 3.1254737046083544E-4 0.0 4.7523765320681415 6.250947409216709E-5 65 0.0 3.1254737046083544E-4 0.0 4.994757017860519 6.250947409216709E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTATA 140 2.8731301E-7 22.818987 10 GCGTAAG 460 0.0 20.062443 38 TTGCGTA 490 0.0 19.557604 36 TGCGTAA 485 0.0 19.028595 37 ATTCGCG 305 1.8189894E-12 18.622995 56 CGCGGAA 290 1.4551915E-11 18.361828 59 CGATCGT 310 1.8189894E-12 18.317757 1 TCGCGCT 335 0.0 18.012716 37 CGTAAGT 500 0.0 17.7481 39 CTCGCGC 355 1.8189894E-12 16.996853 36 CCTCGCG 380 0.0 16.812675 35 CGGTTAC 345 2.0008883E-10 15.431443 4 AATTCGC 400 7.2759576E-12 15.086829 55 CGAACTC 875 0.0 14.60438 25 TCGAACT 965 0.0 14.346736 24 CGCGCTT 400 1.2187229E-10 14.198038 38 GGGCGTA 385 1.036824E-9 13.8286085 34 TCGCGGA 390 1.2551027E-9 13.653238 58 TTAGTCG 290 1.4248017E-6 13.463445 15 CGCTCCG 425 3.1832315E-10 13.363485 25 >>END_MODULE