FastQCFastQC Report
Thu 26 May 2016
SRR936105_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936105_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199515
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69050.2158139593032069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49990.15624243049337164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34690.10842268281286384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13500.042.104603100-101
CGGTATC9850.034.442814100-101
GGTATCA78650.034.198311
CGTACAT18150.033.940926100-101
TCGAGTA4800.033.47977100-101
TACGCAG10750.031.559227100-101
CGAGTAC11050.031.241056100-101
TCGTATC6050.030.989544100-101
GTATCAA154000.030.7965831
ACGTATC4200.029.7598100-101
CCGTACA8500.028.359337100-101
CCTGTCG10800.026.7266296-97
ACCGTAC3900.026.70730298-99
AGTATCA35350.026.434448100-101
CCGGTAT4900.026.115742100-101
GGGTATC15400.024.928661100-101
CGAGCGT3000.024.79789296-97
CCGAGTA7500.024.601435100-101
AGCGTAC4050.024.24853598-99
AGGTATC23950.023.9818100-101
Produced by FastQC (version 0.11.5)