FastQCFastQC Report
Thu 26 May 2016
SRR936104_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936104_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3214366
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66350.2064170663826086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA807.878962E-526.62427123
CGATCGT3500.021.2967641
ATTCGCG3550.019.99851856
CGCGGAA3600.019.72260359
TCGCGCT4000.019.52294537
CTCGCGC3950.018.87055236
GCGTAAG4400.018.55573338
CGTAAGT4700.018.12744539
TTGCGTA4550.017.9423336
CAATTCG4800.017.7489654
AATTCGC4450.017.54953455
TCGCGGA4250.017.54151558
TGCGTAA4700.017.37051237
CGCTCCG4350.017.13612725
TCGCGTA1250.001603965917.03979922
CGCGCTT4450.016.7518338
ACGATCG2301.9141062E-615.43508159
CGGTTAC4251.8189894E-1215.03301054
CCTCGCG4850.014.636723535
CTTGCGT5600.014.57791535
Produced by FastQC (version 0.11.5)