FastQCFastQC Report
Thu 26 May 2016
SRR936104_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936104_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3214366
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68360.21267024352547284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50590.15738717992910578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35950.11184165088854224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13450.044.470974100-101
CGTACAT20300.039.726116100-101
CGGTATC12000.036.949444100-101
CCGTACA8350.034.569107100-101
GGTATCA82700.034.538041
GCGTACA7400.031.36648100-101
GTATCAA155100.029.6571661
TCGAGTA5250.028.907715100-101
CGAGTAC13150.028.513311100-101
ACGTATC4700.028.491646100-101
TCGTATC6550.028.167822100-101
CCGGTAT5700.026.625526100-101
TACGCAG10500.026.073626100-101
TCGTACA11400.025.842422100-101
CCTGTCG10450.025.62785196-97
TCGGTAT4850.025.156199100-101
AGTATCA34550.025.063852100-101
AGCGTAC4750.025.0589398-99
CGAGCGT2850.025.05834496-97
GCGTATC4050.024.247211100-101
Produced by FastQC (version 0.11.5)