FastQCFastQC Report
Thu 26 May 2016
SRR936103_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936103_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581769
Sequences flagged as poor quality0
Sequence length77
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97050.37590504805038716No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37290.14443584999277628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG700.001129035425.35923452
CGGGATA850.003493902720.88124359
TCGCGCT1801.4687976E-719.72155637
CGGTTAC2404.1654857E-1019.2259084
ATACGTA3800.018.6814061
CGGTATA1159.1753085E-418.51967610
CGCGCGT1159.178891E-418.51868
TACGTAG4350.017.9509812
AGCGCGA1602.309904E-517.75008823
CGATCGT2004.4512308E-717.7473371
ACGTAGG4450.017.5475883
GCCTCGA7150.017.37770821
ATTCGCG1854.152578E-617.27068956
TTCGCGG2107.4122727E-716.90386457
CGAACTC6300.016.90288225
CGTAGGC4700.016.6142044
TGCGTAA2159.477426E-716.51106837
GCGTAAG2159.481264E-716.51043138
ATTCGTC1754.9879374E-516.2264534
CGCGCTT2201.2053388E-616.13519338
Produced by FastQC (version 0.11.5)