FastQCFastQC Report
Thu 26 May 2016
SRR936103_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936103_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581769
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106910.41409591640460475No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77070.29851624990461967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56560.21907459575198246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14300.047.238216100-101
CGGTATC10600.043.23331100-101
GGTATCA91950.038.181591
CGTACAT19300.038.084064100-101
CGAGTAC12200.036.831615100-101
TACGCAG11400.036.022835100-101
TCGTATC4700.034.823185100-101
ACGTATC5600.032.414955100-101
GTATCAA180950.031.6351741
CCTGTCG6550.031.34356196-97
ACCGTAC3900.031.28374398-99
CCGTACA9450.031.175041100-101
TCGAGTA4400.031.11063100-101
CCGGTAT5300.030.880936100-101
GCGTATC3750.030.154768100-101
AGTATCA37050.029.31624100-101
CCCGGTA3900.028.99468898-99
AGGTATC24050.028.706255100-101
GCGTACA5250.027.774126100-101
GGGTATC15350.027.334705100-101
Produced by FastQC (version 0.11.5)