FastQCFastQC Report
Thu 26 May 2016
SRR936102_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936102_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589463
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100710.38892233640720103No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36710.14176684509490964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG2103.6379788E-1223.6615565
CGGTATA1806.188202E-921.69269210
TGCGCGT900.004861420519.72329712
TTGCGTA2554.5474735E-1119.48636236
TTACGCG950.0066477518.68342256
CCGATAA1159.172246E-418.520622
ATTTGCG2751.3278623E-1018.0684743
GTTACGC1000.008934461517.74959455
CAACGCG1200.001220644517.74856610
CGTAAGT2902.8012437E-1017.13753939
TGCGTAA2902.8012437E-1017.13588337
GTCGGGT3451.0913936E-1116.47198566
TGCGCGC3055.7480065E-1016.2931376
GCGTAAG3107.239578E-1016.03096238
CCGCCTA1350.002685115715.77497925
TCGCGCT2352.3970206E-615.10457937
CGCCTAG1450.004321848514.68648126
TTTGCGC3654.656613E-1014.585894
ATCGTAC1951.2572945E-414.56039633
CGCCTCG1951.2572945E-414.56039633
Produced by FastQC (version 0.11.5)