##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936102_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2589463 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.447096173994375 39.0 36.0 41.0 30.0 41.0 2 36.306947038826195 39.0 36.0 41.0 29.0 41.0 3 36.16464919560542 39.0 36.0 41.0 29.0 41.0 4 36.059122682965544 39.0 35.0 41.0 28.0 41.0 5 35.98103853965088 39.0 35.0 41.0 28.0 41.0 6 35.87705790737307 39.0 35.0 40.0 28.0 41.0 7 35.72464947365535 39.0 35.0 40.0 27.0 41.0 8 35.57892041709034 38.0 35.0 40.0 27.0 41.0 9 35.727473997504504 39.0 35.0 40.0 28.0 41.0 10-11 35.927306356568906 39.0 35.0 41.0 29.0 41.0 12-13 35.82370244332512 39.0 35.0 41.0 27.5 41.0 14-15 35.66592687364137 39.0 35.0 41.0 27.0 41.0 16-17 35.49822318372574 39.0 35.0 41.0 26.5 41.0 18-19 35.32204283281901 39.0 35.0 41.0 26.0 41.0 20-21 35.07242524801474 39.0 35.0 41.0 24.5 41.0 22-23 34.74579015031301 39.0 34.0 41.0 23.5 41.0 24-25 34.4859996068683 39.0 34.0 41.0 23.0 41.0 26-27 33.529725275085994 38.0 33.0 39.5 20.5 40.5 28-29 33.54211703353167 38.0 33.0 39.5 19.5 40.5 30-31 33.94761230417272 38.0 33.0 40.0 20.0 41.0 32-33 33.82894368446276 38.0 33.0 40.0 19.0 41.0 34-35 33.57567611508641 38.0 33.0 40.0 18.0 41.0 36-37 33.27531422538186 37.5 33.0 40.0 14.5 41.0 38-39 32.97509676716756 37.0 32.0 40.0 13.0 41.0 40-41 32.62041203137484 36.5 32.0 40.0 8.5 41.0 42-43 32.250594042085176 36.0 31.5 39.0 7.5 41.0 44-45 31.892684313311292 35.5 31.0 39.0 6.0 41.0 46-47 31.47733854470985 35.0 31.0 38.5 2.0 40.0 48-49 31.067463601526647 35.0 31.0 37.5 2.0 40.0 50-51 30.61288498812302 35.0 30.0 37.0 2.0 39.0 52-53 30.12420644743717 35.0 30.0 36.5 2.0 39.0 54-55 29.66139716999239 34.0 29.0 36.0 2.0 38.5 56-57 29.216787418858658 34.0 29.0 36.0 2.0 37.5 58-59 28.789827659248267 34.0 29.0 35.0 2.0 37.0 60-61 28.418977988872598 34.0 29.0 35.0 2.0 36.5 62-63 28.045332178911224 34.0 27.5 35.0 2.0 36.0 64-65 27.656357708142576 34.0 27.0 35.0 2.0 36.0 66-67 27.2913411004521 34.0 26.0 35.0 2.0 35.0 68-69 26.918439460227855 33.0 25.5 35.0 2.0 35.0 70-71 26.46098920895954 33.0 25.0 35.0 2.0 35.0 72-73 25.97705837078962 33.0 24.0 35.0 2.0 35.0 74-75 25.40495210783085 33.0 21.0 35.0 2.0 35.0 76-77 23.67301077482088 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9265338659408542 1101 2 0.9969675278970627 1101 3 0.9960100510677989 1101 4 0.9839390080910206 1101 5 1.0139699772365063 1101 6 0.9922075029083786 1101 7 1.023664931473519 1101 8 1.1406365700693257 1101 9 1.0264701084944363 1101 10-11 1.021531176034678 1101 12-13 1.2229680763552295 1101 14-15 1.2635519426907322 1101 16-17 1.2810320348877369 1101 18-19 1.2830794470754157 1101 20-21 1.296881029158122 1101 22-23 1.1388456209029911 1101 24-25 1.225090530269597 1101 26-27 1.2028315380430357 1101 28-29 1.208050567083916 1101 30-31 1.2747572817979105 1101 32-33 1.401077488485079 1101 34-35 1.3657076326630673 1101 36-37 1.1836373550128485 1101 38-39 1.236301243389775 1101 40-41 1.1708324345785783 1101 42-43 1.1308036879787267 1101 44-45 1.0648879596960086 1101 46-47 1.0510439216921235 1101 48-49 1.0265572389546307 1101 50-51 1.062293460805936 1101 52-53 1.2116694653460875 1101 54-55 1.1620957731759667 1101 56-57 1.2313338365322366 1101 58-59 1.2828594403094087 1101 60-61 1.2550240890278737 1101 62-63 1.2059665898010756 1101 64-65 1.1863117925873041 1101 66-67 1.2923387034685767 1101 68-69 1.168666354082692 1101 70-71 1.1486968324127353 1101 72-73 1.3012250136324646 1101 74-75 1.2200283134613983 1101 76-77 1.291159743140529 1102 1 0.41950638401038987 1102 2 0.6872059880175172 1102 3 0.7667176244533707 1102 4 0.6474470021590975 1102 5 0.6337678487866469 1102 6 0.7006195365189569 1102 7 0.7052465903767668 1102 8 0.7005944880623787 1102 9 0.7953088225651541 1102 10-11 0.7432616696006278 1102 12-13 0.88597085247455 1102 14-15 0.8168631837728881 1102 16-17 0.8802426189458288 1102 18-19 0.8899462841427948 1102 20-21 0.9973305493214468 1102 22-23 0.757478808597817 1102 24-25 0.7195768106273803 1102 26-27 0.6709410736964898 1102 28-29 0.8975354992767279 1102 30-31 1.0185190094575702 1102 32-33 0.9285254568829941 1102 34-35 0.8863677451385499 1102 36-37 0.9612549617723829 1102 38-39 1.0170960657484045 1102 40-41 1.00807719461897 1102 42-43 1.0306125890316693 1102 44-45 0.9023755270010767 1102 46-47 0.9724629272416188 1102 48-49 0.880906584269205 1102 50-51 0.7731205840970006 1102 52-53 0.7281967815072825 1102 54-55 0.8564109707096499 1102 56-57 0.8570884821987121 1102 58-59 0.9667169014396073 1102 60-61 0.770035923302629 1102 62-63 0.7687061298291944 1102 64-65 0.8203570510360372 1102 66-67 0.6189663203531062 1102 68-69 0.6451338247876173 1102 70-71 0.5027494610593166 1102 72-73 0.6172022991160269 1102 74-75 0.4357453721505955 1102 76-77 0.5445467497190215 1103 1 0.6899303474104386 1103 2 0.7050246714192809 1103 3 0.6304960820496319 1103 4 0.5828011710104093 1103 5 0.5760745845017183 1103 6 0.5262136606824086 1103 7 0.6901634257656895 1103 8 0.5582423116125099 1103 9 0.4935519264631125 1103 10-11 0.7444864183994042 1103 12-13 0.7709278182165349 1103 14-15 0.6978821485576674 1103 16-17 0.6847761220769328 1103 18-19 0.628487287011744 1103 20-21 0.7034095751957778 1103 22-23 0.5680916064833426 1103 24-25 0.6844677931958856 1103 26-27 0.7225386583466715 1103 28-29 0.6587454462266891 1103 30-31 0.7848617932537891 1103 32-33 0.7687907709483781 1103 34-35 0.8869658352832843 1103 36-37 0.8343366703977892 1103 38-39 0.583322323636672 1103 40-41 0.6362705657837253 1103 42-43 0.7167217227538103 1103 44-45 0.7342260706766197 1103 46-47 0.6905443400989206 1103 48-49 0.6648957161360691 1103 50-51 0.7610603878951991 1103 52-53 0.8207541302689556 1103 54-55 0.7448795944200306 1103 56-57 0.8209761219155283 1103 58-59 0.682493087671336 1103 60-61 0.5067514824522164 1103 62-63 0.47503361770942476 1103 64-65 0.46004244116759807 1103 66-67 0.43906176270117925 1103 68-69 0.5141638465937923 1103 70-71 0.48198074297202 1103 72-73 0.5300544816157 1103 74-75 0.36498050240955493 1103 76-77 0.3061481692714487 1104 1 0.569717100446276 1104 2 0.5402178354416165 1104 3 0.5587211507284167 1104 4 0.7062970924661371 1104 5 0.6074766730749417 1104 6 0.5576763869532968 1104 7 0.525196921502598 1104 8 0.4790170175196735 1104 9 0.43661246924959585 1104 10-11 0.4444636133522408 1104 12-13 0.5993857488364682 1104 14-15 0.7157569224845091 1104 16-17 0.829755337187855 1104 18-19 0.7354083551843402 1104 20-21 0.7403922268822924 1104 22-23 0.6799356977351678 1104 24-25 0.664267777772821 1104 26-27 0.6217438925663643 1104 28-29 0.5772492044457351 1104 30-31 0.8121136598403069 1104 32-33 0.889236517345708 1104 34-35 0.8086028982304896 1104 36-37 0.8547356532663244 1104 38-39 0.8009800211969278 1104 40-41 0.9080325786389167 1104 42-43 0.9269113440845658 1104 44-45 0.9904195194324927 1104 46-47 0.8702157332104825 1104 48-49 0.7723029569362758 1104 50-51 0.6460067004640742 1104 52-53 0.6127341191696196 1104 54-55 0.6620747812368677 1104 56-57 0.6873864954437465 1104 58-59 0.5507562065054081 1104 60-61 0.5065261507903749 1104 62-63 0.46198604941292487 1104 64-65 0.34164371139554106 1104 66-67 0.25588228104804145 1104 68-69 0.035652660256783264 1104 70-71 0.05967246355351108 1104 72-73 0.011333416343173752 1104 74-75 0.09127775367635849 1104 76-77 0.29595914113555466 1105 1 0.037351512933625486 1105 2 0.2505515696724103 1105 3 0.10536419369498873 1105 4 0.14622699563133779 1105 5 -0.1163492823686596 1105 6 0.08971936384921264 1105 7 0.17016909956301163 1105 8 -0.007810790999307926 1105 9 -0.051296084835428246 1105 10-11 0.09242832749342966 1105 12-13 0.1969922361488372 1105 14-15 0.19064942862701884 1105 16-17 0.23232209177665908 1105 18-19 0.23680848526093712 1105 20-21 -0.09204414139966133 1105 22-23 -0.07620745362856951 1105 24-25 -0.0021221965215687533 1105 26-27 -0.2995162115981529 1105 28-29 -0.0696954149253628 1105 30-31 0.1757106649814304 1105 32-33 0.12560750923140773 1105 34-35 0.042143627175626364 1105 36-37 0.026087056235368777 1105 38-39 0.048884342630664435 1105 40-41 0.04026292778103624 1105 42-43 0.0545925609201916 1105 44-45 -0.05082366703128116 1105 46-47 -0.019651777061440612 1105 48-49 -0.1886311556522493 1105 50-51 -0.20539078420546986 1105 52-53 -0.26678533296012574 1105 54-55 -0.3167842224383115 1105 56-57 -0.22442789247358164 1105 58-59 -0.135981787217883 1105 60-61 -0.07608894377325015 1105 62-63 -0.06268817838630625 1105 64-65 -0.12418924340800075 1105 66-67 -0.277462429686377 1105 68-69 -0.2293706709430161 1105 70-71 -0.516462351551894 1105 72-73 -0.9658999524343166 1105 74-75 -1.1548483380379686 1105 76-77 -1.0454438666277461 1106 1 0.1410514551621631 1106 2 0.14910208292528893 1106 3 0.11412154909351813 1106 4 0.14001450190153975 1106 5 0.2025160822098826 1106 6 0.12256903817921483 1106 7 -0.2176979727933741 1106 8 -0.013871563285242416 1106 9 0.06030573848534715 1106 10-11 -0.11361985682736275 1106 12-13 0.14870803604267735 1106 14-15 0.1242529064566682 1106 16-17 0.12457132968159357 1106 18-19 0.18311613703152574 1106 20-21 0.16133185889965773 1106 22-23 0.26701023319173345 1106 24-25 0.12403505601710663 1106 26-27 0.07504550843155045 1106 28-29 0.24809664330523162 1106 30-31 0.4035448278944287 1106 32-33 0.2204246127564815 1106 34-35 0.2286905327240376 1106 36-37 0.14569531685761916 1106 38-39 0.35858237098117485 1106 40-41 0.321691541335575 1106 42-43 0.32358968693238666 1106 44-45 0.24418575967410305 1106 46-47 0.27082106749526247 1106 48-49 0.2096358113076704 1106 50-51 0.058962032676735276 1106 52-53 0.2504719111442988 1106 54-55 0.2578964966674313 1106 56-57 0.2767986414349721 1106 58-59 0.08381510263520298 1106 60-61 -0.1316328643568241 1106 62-63 -0.25135854999444973 1106 64-65 -0.15137227242120233 1106 66-67 -0.30209766406668237 1106 68-69 -0.3347666591553562 1106 70-71 -0.38218934934440085 1106 72-73 -0.35453387601743813 1106 74-75 -0.28889153853920746 1106 76-77 -0.2904428456391379 1107 1 0.0298047278726159 1107 2 0.10446776561897053 1107 3 0.2617043234696581 1107 4 0.13980847355816195 1107 5 0.25650439266718195 1107 6 -0.10899900754468206 1107 7 0.19848801213240108 1107 8 0.15664147639413528 1107 9 0.04956806204389608 1107 10-11 0.18203518156897047 1107 12-13 0.30613061687165555 1107 14-15 0.04892879093863911 1107 16-17 0.1366804349395565 1107 18-19 0.2887964992982859 1107 20-21 0.17754965950344115 1107 22-23 0.21875272747961816 1107 24-25 0.2507234927567623 1107 26-27 0.142653594423642 1107 28-29 0.3833037010244169 1107 30-31 0.5822705862472333 1107 32-33 0.6554048248446591 1107 34-35 0.45871505086280706 1107 36-37 0.5446270790448438 1107 38-39 0.4445004792335183 1107 40-41 0.49193322100730086 1107 42-43 0.4647704943429076 1107 44-45 0.5052524687412472 1107 46-47 0.4000889178215701 1107 48-49 0.43308051942754844 1107 50-51 0.5099419923227551 1107 52-53 0.5683945541647297 1107 54-55 0.4112085439795017 1107 56-57 0.5378569255512744 1107 58-59 0.4789392465836073 1107 60-61 0.3649367254307876 1107 62-63 0.2720618942669937 1107 64-65 0.40145553935253275 1107 66-67 0.523567049236842 1107 68-69 0.2431306661218855 1107 70-71 0.21487948771214604 1107 72-73 0.0929779143183147 1107 74-75 0.11808965145599615 1107 76-77 0.004530307872041561 1108 1 0.2540136372428137 1108 2 0.49259987007365424 1108 3 0.5858272113344754 1108 4 0.4070373522064017 1108 5 0.6113922574905288 1108 6 0.5025616683385721 1108 7 0.6344112072168855 1108 8 0.5120700478227036 1108 9 0.4781579237950524 1108 10-11 0.48105668694531545 1108 12-13 0.5413641038148214 1108 14-15 0.4700870090646063 1108 16-17 0.5009907267982427 1108 18-19 0.43552307379905386 1108 20-21 0.5105680926831582 1108 22-23 0.44907530812478313 1108 24-25 0.44553455266459707 1108 26-27 0.43356910901658097 1108 28-29 0.5304818884284117 1108 30-31 0.6528896338662804 1108 32-33 0.6907289415881337 1108 34-35 0.6355460800486767 1108 36-37 0.6964780775087505 1108 38-39 0.6305557787726883 1108 40-41 0.4131083362146768 1108 42-43 0.4708058246040494 1108 44-45 0.48840761467058513 1108 46-47 0.5360950622148088 1108 48-49 0.5854799049882224 1108 50-51 0.48382217665454874 1108 52-53 0.6125896386501388 1108 54-55 0.6496294349165233 1108 56-57 0.6546126859199362 1108 58-59 0.7191988471980473 1108 60-61 0.8776295057687271 1108 62-63 0.7305872398891147 1108 64-65 0.72671513996697 1108 66-67 0.8013903979311543 1108 68-69 0.7250769026810282 1108 70-71 0.810761208142253 1108 72-73 0.833730602490359 1108 74-75 0.827121909520514 1108 76-77 0.7443828424342556 1109 1 -0.3159742094039686 1109 2 -0.2931723097959775 1109 3 -0.40584265939804 1109 4 -0.2276567147475319 1109 5 -0.010509410833414279 1109 6 -0.2535731233971461 1109 7 -0.20686997718262745 1109 8 -0.23447552725187393 1109 9 -0.15832555869307186 1109 10-11 -0.27910660992795755 1109 12-13 -0.2960087831589959 1109 14-15 -0.10708943552715766 1109 16-17 -0.15140304184610898 1109 18-19 -0.29271071252289715 1109 20-21 -0.2666576282316555 1109 22-23 -0.244432591554812 1109 24-25 -0.2964135193716473 1109 26-27 -0.5549444253995048 1109 28-29 -0.29184581016842515 1109 30-31 -0.2562141115762202 1109 32-33 -0.13231779357925433 1109 34-35 -0.16818190580924153 1109 36-37 -0.2531109181823368 1109 38-39 -0.2662702073061993 1109 40-41 -0.1686572145531997 1109 42-43 -0.16549447370553594 1109 44-45 -0.26381689090890603 1109 46-47 -0.20915850092738353 1109 48-49 -0.14847347032942082 1109 50-51 -0.29967091803111856 1109 52-53 -0.2962376732490988 1109 54-55 -0.1286008158844929 1109 56-57 -0.15708856256089376 1109 58-59 -0.05813338128499268 1109 60-61 -0.07843479651831231 1109 62-63 -0.23604628126876648 1109 64-65 -0.27346097316969775 1109 66-67 -0.14777963852890608 1109 68-69 -0.20483934966678063 1109 70-71 -0.2018223737823952 1109 72-73 -0.3248684473383925 1109 74-75 -0.37772617356423055 1109 76-77 -0.401125278215396 1110 1 -0.3821768389476645 1110 2 -0.39403749256371157 1110 3 -0.5178064882992217 1110 4 -0.3529186917496432 1110 5 -0.38214620404793465 1110 6 -0.39798045620354827 1110 7 -0.4448122360065625 1110 8 -0.6265087067560486 1110 9 -0.5884208307837042 1110 10-11 -0.5408260969374723 1110 12-13 -0.3453611709104507 1110 14-15 -0.42094595796836387 1110 16-17 -0.47453308272556427 1110 18-19 -0.5643377320617518 1110 20-21 -0.753204801089737 1110 22-23 -0.6509979519484759 1110 24-25 -0.6744507953207517 1110 26-27 -0.7048118433643751 1110 28-29 -0.5553203093737835 1110 30-31 -0.4029858240091784 1110 32-33 -0.45673625987706856 1110 34-35 -0.41121582471323137 1110 36-37 -0.34890653787586956 1110 38-39 -0.4121401919232852 1110 40-41 -0.4005546674483256 1110 42-43 -0.3491282413300212 1110 44-45 -0.34852278825981386 1110 46-47 -0.501340835221086 1110 48-49 -0.5080219265136066 1110 50-51 -0.5083316694992952 1110 52-53 -0.5222382001777 1110 54-55 -0.37042917314208523 1110 56-57 -0.3601638708566206 1110 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2314 34-35 -0.6469988292892808 2314 36-37 -1.094599715366364 2314 38-39 -0.7566841906161841 2314 40-41 -0.5961802673868206 2314 42-43 -0.3890063854882584 2314 44-45 -0.4303375789135693 2314 46-47 -0.21590262633256785 2314 48-49 -0.20580135818939738 2314 50-51 -0.14742747584666205 2314 52-53 -0.14560405483249994 2314 54-55 -0.33193279469818293 2314 56-57 -0.29515901127977173 2314 58-59 -0.23482723473357936 2314 60-61 -0.18211610949935064 2314 62-63 -0.1845059210223461 2314 64-65 -0.11224339444712328 2314 66-67 0.001512967391871456 2314 68-69 -0.05396209527302531 2314 70-71 -0.12989655630823194 2314 72-73 -0.08471135349833503 2314 74-75 0.03175855853892884 2314 76-77 0.0662405006828628 2315 1 -1.1479605034068285 2315 2 -1.065443018604995 2315 3 -1.0524048921029205 2315 4 -0.9371556468475291 2315 5 -1.1081195684318956 2315 6 -1.0608870859975994 2315 7 -1.5243891712126327 2315 8 -1.4989088231958974 2315 9 -1.05659515094478 2315 10-11 -1.2651820390086428 2315 12-13 -1.2218106044790886 2315 14-15 -1.091456352650944 2315 16-17 -1.1521663214515243 2315 18-19 -1.152272258903558 2315 20-21 -1.1299459407447827 2315 22-23 -1.206014568885628 2315 24-25 -1.264529149637525 2315 26-27 -1.2081152176942354 2315 28-29 -1.2258088715842064 2315 30-31 -1.2857442355755353 2315 32-33 -1.1790490464282684 2315 34-35 -1.1178541091660819 2315 36-37 -1.35124503822761 2315 38-39 -1.2339387024635151 2315 40-41 -1.1979244610101745 2315 42-43 -1.374892377855744 2315 44-45 -1.3693546054492494 2315 46-47 -1.3635470065332207 2315 48-49 -1.2174377865917236 2315 50-51 -1.1491062370950544 2315 52-53 -1.1503214304132463 2315 54-55 -1.3404847246545906 2315 56-57 -1.3756515840264498 2315 58-59 -1.2570827674345644 2315 60-61 -1.3994591098099534 2315 62-63 -1.2975190357337176 2315 64-65 -1.1388316906858194 2315 66-67 -1.1188234147462346 2315 68-69 -1.3133926652786094 2315 70-71 -1.371381333642674 2315 72-73 -1.171994720751524 2315 74-75 -1.3417380715580087 2315 76-77 -1.2537562304134262 2316 1 -2.286085554655358 2316 2 -1.9970089808957212 2316 3 -2.186518087455106 2316 4 -3.0555134749707946 2316 5 -2.593243264307411 2316 6 -2.4234088515518266 2316 7 -2.1551107988178657 2316 8 -2.040355020675193 2316 9 -2.2218766199569018 2316 10-11 -2.07161053464106 2316 12-13 -2.7128097941493152 2316 14-15 -3.96734204791872 2316 16-17 -3.0778799422268364 2316 18-19 -2.67710488721783 2316 20-21 -2.4120504346986564 2316 22-23 -2.6468658010209865 2316 24-25 -2.7668115785045515 2316 26-27 -2.7568494391747294 2316 28-29 -3.3100481986246137 2316 30-31 -3.6479300519104356 2316 32-33 -3.5091537622581512 2316 34-35 -3.496106401482905 2316 36-37 -3.5072672837093037 2316 38-39 -3.3544692272967467 2316 40-41 -3.142530341689117 2316 42-43 -3.4339537217490914 2316 44-45 -3.3807534854577383 2316 46-47 -3.2715756623022365 2316 48-49 -2.9760882981123906 2316 50-51 -3.0892006791010047 2316 52-53 -3.080320152214494 2316 54-55 -3.2328297611435453 2316 56-57 -3.216587466682981 2316 58-59 -3.4153612748842903 2316 60-61 -3.1021802261356832 2316 62-63 -3.034152814909209 2316 64-65 -2.965432815500723 2316 66-67 -2.896426478564525 2316 68-69 -2.981681880657508 2316 70-71 -2.867302191434618 2316 72-73 -3.0529475093953273 2316 74-75 -3.1757008976795724 2316 76-77 -3.4866164292336244 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70223.0 3 6065.0 4 8432.0 5 10625.0 6 11693.0 7 11958.0 8 14349.0 9 15252.0 10 19482.0 11 21870.0 12 16618.0 13 13234.0 14 12895.0 15 13205.0 16 13590.0 17 14042.0 18 14910.0 19 15509.0 20 16689.0 21 17544.0 22 19947.0 23 22094.0 24 25856.0 25 30119.0 26 35186.0 27 41188.0 28 48461.0 29 57565.0 30 67796.0 31 81765.0 32 98995.0 33 123921.0 34 164075.0 35 217796.0 36 306849.0 37 418134.0 38 385723.0 39 104899.0 40 909.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.107921795800145 26.747535602220612 29.573468501086168 21.57107410089307 2 21.742626507150163 26.961260075829173 29.573338287166006 21.722775129854657 3 22.031662778661808 27.20592779125276 29.025629934032658 21.736779496052776 4 21.675612246947455 27.018317582005015 29.463172482838857 21.842897688208673 5 21.947480571426585 27.065129606863238 29.413900356842284 21.573489464867894 6 22.017235279486215 27.030467635958022 29.35864398086136 21.5936531036944 7 21.926176557493875 26.732521827799378 29.501219575784614 21.840082038922137 8 21.607579804440736 27.160682661351842 29.285492190955143 21.94624534325228 9 21.706099447471132 26.92277535192091 29.396698731752156 21.974426468855796 10-11 22.07496015716194 27.032969707082717 29.179307062939706 21.712763072815637 12-13 22.154354764853647 26.75287659888569 29.102534365746134 21.99023427051453 14-15 21.91868248877907 27.113110749830405 29.158610104758313 21.809596656632213 16-17 22.08920096682109 26.93350218137222 29.230522951959603 21.74677389984708 18-19 21.981086529994283 26.965695437902642 28.98509555222543 22.068122479877644 20-21 21.99596282461692 27.21362481999655 28.858948553918385 21.931463801468144 22-23 22.16453165877493 27.224648096896974 28.4662182870074 22.1446019573207 24-25 22.19396490371514 27.42785419249401 28.178055097779698 22.200125806011147 26-27 22.31551041004639 27.498682462058866 27.981473939205735 22.204333188689006 28-29 22.39341970342706 27.52926046009551 27.997562996162973 22.079756840314456 30-31 22.195045744924492 27.436160060310925 28.12578087314121 22.243013321623376 32-33 22.200971647347398 27.750886273849446 27.902148569583552 22.145993509219604 34-35 22.232712448813476 27.403379251945353 28.083111175052355 22.280797124188812 36-37 22.23171458690866 27.58814397642838 28.152079268797824 22.02806216786513 38-39 22.20923583318962 27.537397613899074 28.29679237692956 21.95657417598175 40-41 22.093449454124396 27.43946549109107 28.232353330812547 22.234731723971986 42-43 22.0335785117918 27.715394718521136 27.9582042407949 22.29282252889217 44-45 22.178655430927883 27.482613464153445 28.141632263106942 22.197098841811734 46-47 22.011422320936976 27.47096574095514 28.17703458039782 22.340577357710067 48-49 22.120646873056156 27.548630287379616 28.0117639265977 22.318958912966526 50-51 22.00842291085833 27.662550494491118 27.955662384392603 22.37336421025795 52-53 21.983852587255907 27.530353659243232 28.09663338033338 22.38916037316748 54-55 21.97704631238577 27.546279262591106 28.044787946948063 22.43188647807506 56-57 21.888190634768804 27.79419611587375 27.85678600072265 22.460827248634793 58-59 22.015316083973886 27.707824860382775 27.859579775229687 22.417279280413652 60-61 21.88983586041472 27.578598314764065 27.832870791496955 22.698695033324256 62-63 21.87861896870739 27.542210839813198 27.946279922280294 22.632890269199123 64-65 21.93083306890717 27.461340830869162 27.920505674234562 22.687320425989096 66-67 21.857532701544454 27.493670201568595 28.03541597059806 22.613381126288896 68-69 21.923028487679908 27.377836271361122 28.019220472994533 22.679914767964437 70-71 21.706615054215487 27.21116179792473 28.14564572106865 22.93657742679113 72-73 21.889315842222935 27.03001045903634 28.126557238056982 22.954116460683743 74-75 21.68064654640994 27.0229793588876 28.049624160695117 23.24674993400734 76-77 21.54342898893683 26.926663006705997 28.378434522356777 23.151473482000394 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 10717.0 1 6392.5 2 2021.5 3 1993.0 4 2011.0 5 2116.0 6 2292.0 7 2438.0 8 2632.0 9 2751.0 10 2811.5 11 2955.5 12 3198.0 13 3357.0 14 3547.5 15 3931.0 16 4501.5 17 4879.0 18 5227.5 19 6152.0 20 7544.0 21 9712.0 22 11064.0 23 12710.0 24 16014.5 25 20451.5 26 23230.0 27 26109.0 28 33574.0 29 41665.5 30 45171.0 31 51079.0 32 61878.5 33 71377.5 34 80232.0 35 84479.0 36 90288.0 37 99210.0 38 103400.5 39 104478.0 40 106572.5 41 110288.0 42 115207.5 43 118506.0 44 117643.5 45 117327.5 46 117938.0 47 119150.0 48 120298.0 49 119697.0 50 116729.0 51 110501.5 52 106641.0 53 103622.5 54 95932.0 55 85036.0 56 73879.0 57 68946.0 58 65564.5 59 56254.5 60 46021.0 61 41716.0 62 36735.0 63 26811.0 64 18820.0 65 15772.0 66 13465.5 67 9654.5 68 6949.5 69 4871.5 70 3994.0 71 3327.5 72 2312.5 73 1741.5 74 1519.0 75 1405.5 76 1018.0 77 607.5 78 471.0 79 387.0 80 273.5 81 203.0 82 139.0 83 116.0 84 96.0 85 66.5 86 46.5 87 36.0 88 38.5 89 43.5 90 37.5 91 29.0 92 29.5 93 26.5 94 22.0 95 23.5 96 26.0 97 27.0 98 22.5 99 46.0 100 75.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0033983880055440067 2 0.008573206104895108 3 0.011701267791816296 4 0.018343571620834127 5 0.014056968568386573 6 0.013709406158728663 7 0.017300884391860397 8 0.018459425757386766 9 0.017687064847035854 10-11 0.020892362624992132 12-13 0.01975313028222454 14-15 0.008399424900066153 16-17 0.005889251941425693 18-19 0.011701267791816296 20-21 0.01067788958560134 22-23 0.013999041500110254 24-25 0.021490942330514087 26-27 0.013651479090452345 28-29 0.008418733922824925 30-31 0.008264261740754744 32-33 0.009731747470421473 34-35 0.01083236176767152 36-37 0.009557966265592518 38-39 0.016509214458750716 40-41 0.012357774565614571 42-43 0.015312055047706804 44-45 0.017860846051864807 46-47 0.015814089639434894 48-49 0.01396042345459271 50-51 0.026665760429865185 52-53 0.031396471005764516 54-55 0.024773476199505456 56-57 0.014925874592531347 58-59 0.016219579117369123 60-61 0.01961796712291313 62-63 0.02598994463330814 64-65 0.020216546828435086 66-67 0.020004147578088584 68-69 0.029542804820922332 70-71 0.05626649231906383 72-73 0.06752365258742836 74-75 0.07932146549303852 76-77 0.07737125419440247 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2589463.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.88167979232167 #Duplication Level Percentage of deduplicated Percentage of total 1 76.8477112335128 43.71226903157475 2 12.727100289108687 14.478776866596895 3 4.454980883193452 7.602203882361729 4 2.0696594218424487 4.7090281804961505 5 1.0972068078981707 3.1205483156409564 6 0.6454071650084561 2.2027106217408674 7 0.4299765144630558 1.7120450489734276 8 0.2963822446830661 1.3486975950553357 9 0.2126732489235794 1.0887490481097384 >10 1.089658842555764 11.328660190060301 >50 0.08362688423865014 3.2789037316620853 >100 0.044458685919197806 4.479496211701306 >500 0.0010215693992810186 0.4055591734637917 >1k 6.810462661855405E-5 0.14221107324314136 >5k 0.0 0.0 >10k+ 6.810462661855405E-5 0.3901410293194434 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10071 0.38892233640720103 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3671 0.14176684509490964 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.5447218207018213E-4 0.0 2 0.0 0.0 0.0 3.861804551754553E-4 0.0 3 0.0 0.0 0.0 5.792706827631829E-4 0.0 4 0.0 0.0 0.0 0.0010813052744912748 0.0 5 0.0 0.0 0.0 0.001892284230359731 0.0 6 0.0 0.0 0.0 0.0037459504152019163 0.0 7 0.0 0.0 0.0 0.007067102329710832 0.0 8 0.0 0.0 0.0 0.011006142972500476 0.0 9 0.0 0.0 0.0 0.016219579117369123 0.0 10 0.0 0.0 0.0 0.02127854308016759 0.0 11 0.0 0.0 0.0 0.02757328449952751 0.0 12 0.0 0.0 0.0 0.03568307405821207 0.0 13 0.0 0.0 0.0 0.04676645312174764 0.0 14 0.0 0.0 0.0 0.060784803644616664 0.0 15 0.0 0.0 0.0 0.07681129253439806 0.0 16 0.0 0.0 0.0 0.09279916337866191 0.0 17 0.0 0.0 0.0 0.10774434699395202 0.0 18 0.0 0.0 0.0 0.1230757110644176 0.0 19 0.0 0.0 0.0 0.14006765109213765 0.0 20 0.0 0.0 0.0 0.1592222016688402 0.0 21 0.0 0.0 0.0 0.1795739116565867 0.0 22 0.0 0.0 0.0 0.2017020517381403 0.0 23 0.0 0.0 0.0 0.226031420414194 0.0 24 0.0 0.0 0.0 0.25036078909024767 0.0 25 0.0 0.0 0.0 0.27882228863667874 0.0 26 0.0 0.0 0.0 0.3090988363224344 0.0 27 0.0 0.0 0.0 0.33848716896128656 0.0 28 0.0 0.0 0.0 0.3682616820553142 0.0 29 0.0 0.0 0.0 0.3980361951493418 0.0 30 0.0 0.0 0.0 0.43047535338408 0.0 31 0.0 0.0 0.0 0.4664287537609149 0.0 32 0.0 0.0 0.0 0.5054329797336359 0.0 33 0.0 0.0 0.0 0.5496892598967431 0.0 34 0.0 0.0 0.0 0.5951813175164117 0.0 35 0.0 0.0 0.0 0.6405575209995277 0.0 36 0.0 0.0 0.0 0.6915333410826878 0.0 37 0.0 0.0 0.0 0.7459075491713919 0.0 38 0.0 0.0 0.0 0.8005520835787189 0.0 39 0.0 0.0 0.0 0.8529953893915456 0.0 40 0.0 0.0 0.0 0.9065972365698989 0.0 41 0.0 0.0 0.0 0.9662234988489892 0.0 42 0.0 0.0 0.0 1.0354656544619483 0.0 43 0.0 0.0 0.0 1.1093805935825305 0.0 44 0.0 0.0 0.0 1.191173613988692 0.0 45 0.0 0.0 0.0 1.2722328915300198 0.0 46 0.0 0.0 0.0 1.3617495210396904 0.0 47 0.0 0.0 0.0 1.4536218513259314 0.0 48 0.0 0.0 0.0 1.5485836252535758 0.0 49 0.0 0.0 0.0 1.6435067811357027 0.0 50 0.0 0.0 0.0 1.742214505478549 0.0 51 0.0 0.0 0.0 1.8475259156048958 0.0 52 0.0 0.0 0.0 1.956428803964374 0.0 53 0.0 0.0 0.0 2.0791955706646514 0.0 54 0.0 0.0 0.0 2.2136636051567447 0.0 55 0.0 0.0 0.0 2.349328799059882 0.0 56 0.0 0.0 0.0 2.5005570653065905 0.0 57 0.0 0.0 0.0 2.6559560804691937 0.0 58 0.0 0.0 0.0 2.814599011455271 0.0 59 0.0 0.0 0.0 2.973241942441348 0.0 60 0.0 0.0 0.0 3.1281775410577404 0.0 61 0.0 0.0 0.0 3.297826615016318 0.0 62 0.0 0.0 0.0 3.4730366875294223 0.0 63 0.0 0.0 0.0 3.651490675866 0.0 64 0.0 0.0 0.0 3.847863437322719 0.0 65 0.0 0.0 0.0 4.056246410935395 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCGCG 210 3.6379788E-12 23.661556 5 CGGTATA 180 6.188202E-9 21.692692 10 TGCGCGT 90 0.0048614205 19.723297 12 TTGCGTA 255 4.5474735E-11 19.486362 36 TTACGCG 95 0.00664775 18.683422 56 CCGATAA 115 9.172246E-4 18.5206 22 ATTTGCG 275 1.3278623E-10 18.068474 3 GTTACGC 100 0.0089344615 17.749594 55 CAACGCG 120 0.0012206445 17.748566 10 CGTAAGT 290 2.8012437E-10 17.137539 39 TGCGTAA 290 2.8012437E-10 17.135883 37 GTCGGGT 345 1.0913936E-11 16.471985 66 TGCGCGC 305 5.7480065E-10 16.293137 6 GCGTAAG 310 7.239578E-10 16.030962 38 CCGCCTA 135 0.0026851157 15.774979 25 TCGCGCT 235 2.3970206E-6 15.104579 37 CGCCTAG 145 0.0043218485 14.686481 26 TTTGCGC 365 4.656613E-10 14.58589 4 ATCGTAC 195 1.2572945E-4 14.560396 33 CGCCTCG 195 1.2572945E-4 14.560396 33 >>END_MODULE