FastQCFastQC Report
Thu 26 May 2016
SRR936102_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936102_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2589463
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108610.419430592366062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76970.29724309634854795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55640.21487080525962335No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12900.049.142544100-101
CGGTATC10050.040.867752100-101
TCGAGTA5350.040.61036100-101
GGTATCA91450.040.0237541
CGTACAT20100.039.090893100-101
TACGCAG11900.036.7653100-101
CGAGTAC12900.036.22244100-101
ACGTATC5300.033.693268100-101
GCGTATC3650.032.61631100-101
CCGTACA8750.031.973307100-101
GCGTACA5900.031.780174100-101
TCGTATC4700.031.662113100-101
GACGCAG10550.030.749765100-101
CCGTATC6550.030.443968100-101
GTATCAA181300.030.2663151
TCGTACA11100.030.030514100-101
CCGGTAT5050.029.46771100-101
ACCGTAC3350.029.31449198-99
AGTATCA36100.029.10283100-101
AGGTATC23950.028.457565100-101
Produced by FastQC (version 0.11.5)