FastQCFastQC Report
Thu 26 May 2016
SRR936101_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936101_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4236723
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46220.10909375005163188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG3700.022.06645256
TCGCGCT3500.021.29766737
CGCGGAA3150.020.28492759
CGCTCCG4000.019.5230925
CAATTCG3800.018.68436854
TCGCGGA4000.018.63655758
CTCGCGC4000.018.63435736
CGTAATA1653.0124658E-517.21023615
CGCGCTT4150.017.10575138
CGATCGT4250.016.702871
CCTCGCG5100.015.31114335
CGCACGA3502.4738256E-1015.2102854
AATTCGC4750.014.9457355
CGATTCG6050.014.66781415
CGAGCTA4302.5465852E-1114.03599761
CTCCGCG5900.013.83713927
CGTCGAG1800.001133215813.80404437
TAAGGCG11950.013.66441917
CGAATCG2901.4266752E-613.46213428
GATCGTT5350.013.2681652
Produced by FastQC (version 0.11.5)