FastQCFastQC Report
Thu 26 May 2016
SRR936101_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936101_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4236723
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54700.12910921955483048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74600.035.4961131
CGGTATC12050.033.34299100-101
CGTATCA15350.033.15473100-101
CCGTACA9500.030.074987100-101
GTATCAA137300.029.5145511
CCTGTCG13800.027.81727496-97
ACCGTAC3550.026.82697798-99
CGTACAT26900.026.553194100-101
TCGTATA5400.026.454847100-101
CGATATA3100.025.921484100-101
TACGCAG10250.024.680437100-101
TCGTACA12650.024.46812100-101
ATCAACG170750.023.5544973
TATCAAC172950.023.3586272
GCGTACA8800.023.335882100-101
TCAACGC173200.023.220764
TCGGTAT5300.023.023205100-101
CGAGTAC15000.022.420485100-101
CAACGCA179150.022.4158025
CGAGCCG7200.022.31851296-97
Produced by FastQC (version 0.11.5)