FastQCFastQC Report
Thu 26 May 2016
SRR936100_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936100_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4249877
Sequences flagged as poor quality0
Sequence length77
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47870.11263855400991604No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGA3751.8189894E-1216.09370458
CGCACGA3401.6007107E-1015.6591764
CGCGGAA3201.1332304E-915.53215759
ATTCGCG4059.094947E-1214.90035156
CAATTCG4101.8007995E-1013.852509554
TCGCGCT4152.1827873E-1013.685451537
TCGAACT13250.013.66350124
ATTCGTC3657.2759576E-913.61354834
CTCGCGC4001.8371793E-913.310336
GCCTCGA13900.013.02486721
CTCGAAC14750.012.99614423
TAATTCG4701.1459633E-1012.83754835
CCTCGAA16150.012.748919522
ACGCACG4203.805326E-912.6760293
GACGTAA4559.367795E-1012.48057231
GTTATCG3457.373819E-712.347383523
CGAACTC14250.012.20571725
GTAACGT3508.793104E-712.1689872
AATTCGC4407.579729E-912.10169855
CGGTTAC4154.37376E-811.9739254
Produced by FastQC (version 0.11.5)