FastQCFastQC Report
Thu 26 May 2016
SRR936100_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936100_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4249877
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55130.12972140134879198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78400.035.527441
CGTATCA14350.033.39236100-101
GTATCAA141650.029.0752641
CCGTACA9800.027.636843100-101
GCGTACA9250.027.34956100-101
CGGTATC12450.026.535471100-101
CCTGTCG12950.026.42713596-97
TCGAGTA5750.025.880657100-101
CGTACAT24600.025.286243100-101
TACGCAG10200.024.510506100-101
GCGTATC4150.023.66677100-101
CCGGTAT5350.023.365154100-101
ATCAACG173800.023.2437343
TATCAAC176700.023.0984952
TCAACGC174650.023.0965464
CGAGTAC15700.022.36946100-101
CAACGCA181200.022.2608665
ATACACG10850.022.21658596-97
ACCGTAC3900.022.12971798-99
CCCGTAC5650.021.06948998-99
Produced by FastQC (version 0.11.5)