FastQCFastQC Report
Thu 26 May 2016
SRR936099_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936099_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3558124
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60000.16862818721326184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGTA4450.019.14369836
GCGTAAG4750.018.681938
TAACGCG1401.6732662E-417.75254452
TGCGTAA4900.017.38682637
CGGTATA1250.001605516117.03741310
CGTAAGT5600.016.48033539
GCGTTCG1300.002087002216.38212810
CGGTTAC2602.3333996E-816.3802854
CGAACTC7850.015.37454225
CGCGGAA2151.7305569E-514.85967259
CGCTAAA2151.731707E-514.8586251
TCGAACT8000.014.64358524
ACGCGAA1800.001131952213.80598254
CGTTCGG1800.001133515613.8034611
ATAACGC2102.3508612E-413.52593951
CTCGCGC2658.322942E-613.39456936
TTAGCGG3202.9604416E-713.31160142
CTTGCGT7000.013.18463835
TCGCGGA2701.0088032E-513.14707658
AACGCGA1900.001701466213.079535553
Produced by FastQC (version 0.11.5)