##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936099_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3558124 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.45164811569243 39.0 36.0 41.0 30.0 41.0 2 36.31069574865856 39.0 36.0 41.0 29.0 41.0 3 36.222932365482485 39.0 36.0 41.0 29.0 41.0 4 36.10248883962448 39.0 36.0 41.0 29.0 41.0 5 36.03393052069011 39.0 35.0 41.0 28.0 41.0 6 35.93862805231071 39.0 35.0 40.0 28.0 41.0 7 35.77996045106916 39.0 35.0 40.0 27.0 41.0 8 35.604024199269055 39.0 35.0 40.0 27.0 41.0 9 35.52401068653032 38.0 35.0 40.0 27.0 41.0 10-11 35.7421572435362 39.0 35.0 40.0 27.5 41.0 12-13 35.931123957456236 39.0 35.0 41.0 28.5 41.0 14-15 35.88684739486314 39.0 35.0 41.0 28.0 41.0 16-17 35.73508357212958 39.0 35.0 41.0 27.0 41.0 18-19 35.595855147262995 39.0 35.0 41.0 27.0 41.0 20-21 35.407313938468704 39.0 35.0 41.0 26.5 41.0 22-23 35.168579144515476 39.0 35.0 41.0 25.5 41.0 24-25 34.95661112428909 39.0 34.0 41.0 24.0 41.0 26-27 34.03649282599483 38.0 33.0 40.0 23.5 40.5 28-29 34.07686325715461 38.0 33.5 40.0 23.0 40.5 30-31 34.460566017373196 38.0 34.0 40.0 23.0 41.0 32-33 34.31539949141739 38.0 34.0 40.0 23.0 41.0 34-35 34.0465474221809 38.0 33.0 40.0 22.0 41.0 36-37 33.76305308640171 38.0 33.0 40.0 21.5 41.0 38-39 33.48892955950945 37.0 33.0 40.0 20.0 41.0 40-41 33.11289137197018 37.0 32.0 40.0 18.5 41.0 42-43 32.75948688128913 36.0 32.0 39.0 18.0 41.0 44-45 32.387566031987646 36.0 32.0 39.0 15.0 41.0 46-47 31.9971039514081 35.0 31.0 39.0 12.0 40.0 48-49 31.525335401464368 35.0 31.0 38.0 8.0 40.0 50-51 31.08300666306177 35.0 31.0 37.0 7.0 39.0 52-53 30.622346073380243 35.0 30.0 37.0 2.0 39.0 54-55 30.161046944963132 34.5 30.0 36.0 2.0 39.0 56-57 29.703093680827315 34.0 29.5 36.0 2.0 37.5 58-59 29.267425755819637 34.0 29.0 35.0 2.0 37.0 60-61 28.91159386238366 34.0 29.0 35.0 2.0 36.5 62-63 28.56273502553593 34.0 29.0 35.0 2.0 36.0 64-65 28.189691112507603 34.0 28.5 35.0 2.0 36.0 66-67 27.803720162647508 34.0 27.0 35.0 2.0 35.0 68-69 27.443828967174838 34.0 26.5 35.0 2.0 35.0 70-71 27.020894578154106 33.0 26.0 35.0 2.0 35.0 72-73 26.541719456657496 33.0 25.0 35.0 2.0 35.0 74-75 25.957431359896397 33.0 24.5 35.0 2.0 35.0 76-77 24.255308134286494 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9516158776667396 1101 2 1.0122151913727109 1101 3 1.0066507494345984 1101 4 0.9526997165585769 1101 5 1.0630685818073147 1101 6 0.9935853528221941 1101 7 1.0218332421089826 1101 8 1.1685029179914181 1101 9 1.0620914499029226 1101 10-11 0.9086454339022154 1101 12-13 1.0601600617043445 1101 14-15 1.1339662400887818 1101 16-17 1.1377048412554274 1101 18-19 1.0125439158479352 1101 20-21 0.8549906442927337 1101 22-23 0.8831635735783365 1101 24-25 0.7858041951155954 1101 26-27 0.6886181758089549 1101 28-29 0.6587435932125985 1101 30-31 0.7110493278138605 1101 32-33 0.8129929006711407 1101 34-35 0.88078807941001 1101 36-37 0.7836996788298194 1101 38-39 0.865140008779612 1101 40-41 0.7546148833638213 1101 42-43 0.7001974929775372 1101 44-45 0.5641667089740281 1101 46-47 0.6365568785717386 1101 48-49 0.8481410399539335 1101 50-51 0.8145277678351839 1101 52-53 0.87163643786565 1101 54-55 0.7892975317901048 1101 56-57 0.7677221124725655 1101 58-59 0.9152884675002397 1101 60-61 0.8851503824679376 1101 62-63 0.8383969839950893 1101 64-65 0.8684941499240111 1101 66-67 0.8045710450220973 1101 68-69 0.8881643065213574 1101 70-71 0.7660086740928946 1101 72-73 0.728044370862289 1101 74-75 0.7022245315224325 1101 76-77 0.6960822542805296 1102 1 0.9306156322875481 1102 2 1.0965905239649771 1102 3 0.9664469960199895 1102 4 1.0178201740872836 1102 5 1.0366777548498973 1102 6 1.0445506834041254 1102 7 0.7882381709785875 1102 8 0.9853417689073041 1102 9 0.945811280088698 1102 10-11 0.8577996517634574 1102 12-13 0.9027616945106587 1102 14-15 0.9372839218763218 1102 16-17 0.8932591321391499 1102 18-19 1.007839582983685 1102 20-21 1.0130081874262515 1102 22-23 1.0583864843076185 1102 24-25 1.1221728640369903 1102 26-27 0.9672307191198541 1102 28-29 0.9564088693972224 1102 30-31 1.0878035672566426 1102 32-33 0.9710679083577176 1102 34-35 1.0169770376423344 1102 36-37 0.9618861714447959 1102 38-39 0.9987851904784648 1102 40-41 1.0040391291923711 1102 42-43 1.1296398909530083 1102 44-45 1.1838670359491772 1102 46-47 0.9169500839334219 1102 48-49 0.9535779627215781 1102 50-51 1.1348329692826162 1102 52-53 1.0796328059580809 1102 54-55 1.1591373627445414 1102 56-57 1.31286763267644 1102 58-59 1.218831018615976 1102 60-61 1.0708006151347114 1102 62-63 1.024147363889366 1102 64-65 1.0514592543432002 1102 66-67 1.092592808381955 1102 68-69 1.0538091142386783 1102 70-71 1.119697270169194 1102 72-73 1.2689375141400845 1102 74-75 1.3313455345024572 1102 76-77 1.4797333856855133 1103 1 0.41359931591945553 1103 2 0.3339040425792845 1103 3 0.4080885883907257 1103 4 0.468920198744442 1103 5 0.4753125637198039 1103 6 0.43876728082680216 1103 7 0.4739035528772604 1103 8 0.6198597584400645 1103 9 0.4893490639473441 1103 10-11 0.5661352098032708 1103 12-13 0.5414675549437007 1103 14-15 0.5871003835919097 1103 16-17 0.5635329286192885 1103 18-19 0.5303283065027316 1103 20-21 0.6322033720490836 1103 22-23 0.6061024986718095 1103 24-25 0.546344068177909 1103 26-27 0.5019261289942207 1103 28-29 0.4293006819212053 1103 30-31 0.7547548109602644 1103 32-33 0.6890913678875208 1103 34-35 0.7807294778672542 1103 36-37 0.6933219228486749 1103 38-39 0.7625600631603646 1103 40-41 0.6963881318749969 1103 42-43 0.7862877310411065 1103 44-45 0.7425927518875284 1103 46-47 0.5175798149107962 1103 48-49 0.5913247892704838 1103 50-51 0.7049312456912808 1103 52-53 0.7444081476822646 1103 54-55 0.7684729263461172 1103 56-57 0.76856779415305 1103 58-59 0.7305515602578474 1103 60-61 0.7396006362694187 1103 62-63 0.6956533696698983 1103 64-65 0.5803011370381341 1103 66-67 0.6223031145201077 1103 68-69 0.6006897513204557 1103 70-71 0.6024532921715426 1103 72-73 0.6052190006889511 1103 74-75 0.6865057875257428 1103 76-77 0.722277420969359 1104 1 0.6667175227824842 1104 2 0.7095604267872275 1104 3 0.5405230721700249 1104 4 0.5474731324291682 1104 5 0.6020397601645939 1104 6 0.22091710628433958 1104 7 0.5442451835967361 1104 8 0.7141363135380416 1104 9 0.6922305420796349 1104 10-11 0.6277081693672599 1104 12-13 0.7598093811833095 1104 14-15 0.6717826022567479 1104 16-17 0.7837153597765081 1104 18-19 0.800483378417475 1104 20-21 0.646204243743064 1104 22-23 0.7015686037611673 1104 24-25 0.7997791806087875 1104 26-27 0.6831032417319491 1104 28-29 0.6776454816963593 1104 30-31 0.8189519495772544 1104 32-33 0.7190185773669882 1104 34-35 0.7613167715018534 1104 36-37 0.7426025037607502 1104 38-39 0.7085788521031589 1104 40-41 0.7472598937423243 1104 42-43 0.8258825970079755 1104 44-45 0.6345670572818491 1104 46-47 0.5593600258281484 1104 48-49 0.7596641036193539 1104 50-51 0.7404095979452627 1104 52-53 0.8083490734429937 1104 54-55 0.6590537433759245 1104 56-57 0.5699379287225064 1104 58-59 0.578229283317139 1104 60-61 0.5326315651123075 1104 62-63 0.5177189042206152 1104 64-65 0.558120327068778 1104 66-67 0.47298831758271476 1104 68-69 0.4292859302594074 1104 70-71 0.46252481995780315 1104 72-73 0.4452949276784679 1104 74-75 0.46750761102264704 1104 76-77 0.3928512318606465 1105 1 0.16071458795570237 1105 2 0.2948719530203192 1105 3 0.20345663212547294 1105 4 0.3568449598515002 1105 5 0.29551601674727834 1105 6 0.013232980213473411 1105 7 0.16085202765503226 1105 8 0.36776578637770285 1105 9 0.18547859300627323 1105 10-11 0.2226521912412167 1105 12-13 0.23967889751752836 1105 14-15 0.059064799576340477 1105 16-17 0.17049122029833796 1105 18-19 0.21598865072471796 1105 20-21 0.1845742888035602 1105 22-23 0.27028657007399914 1105 24-25 0.2747263959543389 1105 26-27 0.09697760017845525 1105 28-29 0.25497750772974825 1105 30-31 0.09364505079886953 1105 32-33 0.31970051111521514 1105 34-35 0.2609029398110039 1105 36-37 0.30702775805082894 1105 38-39 0.3705437256751125 1105 40-41 0.47110465759258346 1105 42-43 0.31232120757511694 1105 44-45 0.06929359758206033 1105 46-47 -0.017786022272744617 1105 48-49 0.04154814629046655 1105 50-51 0.18725677053524592 1105 52-53 0.11692622789265172 1105 54-55 0.10368272883726704 1105 56-57 0.17547204784339598 1105 58-59 0.09521859618410389 1105 60-61 -0.12985776798877424 1105 62-63 -0.11457039263500235 1105 64-65 -0.06804485019088702 1105 66-67 -0.09662549229048167 1105 68-69 -0.2728838709359884 1105 70-71 -0.5029538840733601 1105 72-73 -0.4704915988625338 1105 74-75 -0.4809806804612542 1105 76-77 -0.3156254997839305 1106 1 0.034272002201376495 1106 2 0.15871721706801623 1106 3 -0.23280176534750296 1106 4 -0.028373263220970557 1106 5 -0.25338864134766226 1106 6 -0.08625761016780586 1106 7 0.11661169309081032 1106 8 0.14205016596572762 1106 9 -0.05918528562521885 1106 10-11 -0.14816286414873758 1106 12-13 -0.11390031679042778 1106 14-15 0.0902253192186464 1106 16-17 0.3100489904634003 1106 18-19 0.1942737866414248 1106 20-21 -0.00960731906225476 1106 22-23 0.09962900901158633 1106 24-25 0.20908974515574386 1106 26-27 0.1255619308275655 1106 28-29 0.1675167700868201 1106 30-31 0.13993236672180842 1106 32-33 0.08189186227417622 1106 34-35 0.10088603364025062 1106 36-37 0.017953593349126606 1106 38-39 0.02661037157981383 1106 40-41 0.06329489650346432 1106 42-43 0.041253226972720825 1106 44-45 -0.1504267832516888 1106 46-47 0.09403739722274196 1106 48-49 -0.10975095946208313 1106 50-51 -0.17744181023621053 1106 52-53 -0.17111399099776747 1106 54-55 -0.02616778502133954 1106 56-57 -0.048492894097254435 1106 58-59 -0.23001675550873912 1106 60-61 -0.20082920545818794 1106 62-63 -0.1969695117345296 1106 64-65 -0.13212902512526625 1106 66-67 -0.08769814433479084 1106 68-69 -0.23683190670588772 1106 70-71 -0.35368774535377767 1106 72-73 -0.2862287968871797 1106 74-75 -0.40737365995139996 1106 76-77 -0.414539278313093 1107 1 0.2252537731755737 1107 2 0.1333696755523519 1107 3 0.2097254159150097 1107 4 0.1861493743081013 1107 5 0.26224071982505137 1107 6 0.025353792439432254 1107 7 0.010528499495308097 1107 8 0.05981508185256246 1107 9 0.08843844997277017 1107 10-11 0.10025169743507689 1107 12-13 0.19971465589306803 1107 14-15 0.13241821152134747 1107 16-17 0.16642157660501056 1107 18-19 0.2454546164137028 1107 20-21 0.1309162921239846 1107 22-23 0.181752663471471 1107 24-25 -0.12463016613326516 1107 26-27 -0.07906726806895392 1107 28-29 0.019538190544444944 1107 30-31 0.21645208327939258 1107 32-33 0.20590224795059697 1107 34-35 0.15064965131753638 1107 36-37 0.16991226701170348 1107 38-39 0.16298295772143234 1107 40-41 0.18101653873855383 1107 42-43 0.2084420623705654 1107 44-45 0.24791583588609 1107 46-47 0.1630264165336044 1107 48-49 0.3434139672431691 1107 50-51 0.24047672711435908 1107 52-53 0.28864475839369774 1107 54-55 0.27380192342242893 1107 56-57 0.3782577955765696 1107 58-59 0.28512685892302514 1107 60-61 0.2683664455398116 1107 62-63 0.4091461370997891 1107 64-65 0.4209287992918931 1107 66-67 0.5290779686094389 1107 68-69 0.38547890629784476 1107 70-71 0.4228698356490881 1107 72-73 0.3841244242952726 1107 74-75 0.4147911044776613 1107 76-77 0.36523461685648684 1108 1 -0.007280578879793609 1108 2 0.14534884328990216 1108 3 0.14833200649278666 1108 4 0.0548793094797162 1108 5 0.0897785363851824 1108 6 0.15496147068665778 1108 7 0.007534427190989845 1108 8 0.10485964751469368 1108 9 -0.02613833558363865 1108 10-11 -0.15400479105803555 1108 12-13 -0.03508295329022815 1108 14-15 0.09836465870768762 1108 16-17 -0.001674960740700726 1108 18-19 0.033196314452808906 1108 20-21 3.1285146981474554E-4 1108 22-23 0.01226703448395483 1108 24-25 0.09445557470532862 1108 26-27 -0.03448388247677059 1108 28-29 0.054626900371090414 1108 30-31 0.10466256890632053 1108 32-33 0.21099899707316183 1108 34-35 0.2869841024762394 1108 36-37 0.15316520053227833 1108 38-39 0.11413191908962972 1108 40-41 0.22289933772942305 1108 42-43 0.11219297989890009 1108 44-45 0.08141062679243305 1108 46-47 0.1479484394754067 1108 48-49 0.1893996034269918 1108 50-51 0.2603431637735518 1108 52-53 0.23016280774387 1108 54-55 0.19571215059936264 1108 56-57 0.06797810621002753 1108 58-59 0.15836389889950553 1108 60-61 0.08830130873813502 1108 62-63 0.12100481820291975 1108 64-65 -0.011044117944479126 1108 66-67 -0.043118404043799075 1108 68-69 -0.04775648880990602 1108 70-71 -0.09307511059454754 1108 72-73 -0.021800721841035653 1108 74-75 -0.044959780153302376 1108 76-77 -0.057766322774778445 1109 1 0.1384145350545012 1109 2 0.2436214227407234 1109 3 0.005864566291364781 1109 4 0.1707470740403494 1109 5 0.2205196082824088 1109 6 0.1983154334094337 1109 7 -0.04348443411667091 1109 8 0.05988511323106138 1109 9 0.16180429200351654 1109 10-11 -0.06780421259268365 1109 12-13 0.00466945811812991 1109 14-15 0.1607673984956719 1109 16-17 0.23653946686863492 1109 18-19 0.056961010270335066 1109 20-21 0.07276008187367466 1109 22-23 -0.10024754612948072 1109 24-25 -0.04825786551617739 1109 26-27 -0.13432613552512152 1109 28-29 -0.06470963998290813 1109 30-31 0.06491276974307425 1109 32-33 0.15199707388933348 1109 34-35 0.1950999317101889 1109 36-37 0.1543506700168038 1109 38-39 0.1473424932292673 1109 40-41 0.16285798588931044 1109 42-43 0.1769491076340941 1109 44-45 0.11498185402273009 1109 46-47 -0.1117843980537998 1109 48-49 -0.01110739253276094 1109 50-51 -0.009781184808030474 1109 52-53 0.15311994431180054 1109 54-55 0.10295622466010101 1109 56-57 0.11251962005015059 1109 58-59 0.14468920390565287 1109 60-61 0.01474550681521336 1109 62-63 0.03391562742012866 1109 64-65 0.03469029395220957 1109 66-67 0.0151415598957918 1109 68-69 0.04099213067977914 1109 70-71 0.07905645447741705 1109 72-73 0.02074450612184009 1109 74-75 -0.020208145479699624 1109 76-77 0.062082095172034 1110 1 -0.32547549357389727 1110 2 -0.5136256923227265 1110 3 -0.4157320552416195 1110 4 -0.45410371312423337 1110 5 -0.29526066668788786 1110 6 -0.41348161480480883 1110 7 -0.5466391865512961 1110 8 -0.48435468982195573 1110 9 -0.3770312849884476 1110 10-11 -0.4736544024407223 1110 12-13 -0.3654248937050326 1110 14-15 -0.5350747387438517 1110 16-17 -0.5502075956272421 1110 18-19 -0.35136706630526504 1110 20-21 -0.5875692842357765 1110 22-23 -0.6840557321572973 1110 24-25 -0.626208720879049 1110 26-27 -0.502158918527094 1110 28-29 -0.5707785202562974 1110 30-31 -0.560965643776747 1110 32-33 -0.34073289995713907 1110 34-35 -0.13497560743311965 1110 36-37 -0.28877144399994137 1110 38-39 -0.20135522645436055 1110 40-41 -0.10193548400807373 1110 42-43 -0.21475156513785265 1110 44-45 -0.42889334868807794 1110 46-47 -0.3638679116520578 1110 48-49 -0.340306378955443 1110 50-51 -0.2812541097145491 1110 52-53 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-0.38988743008307836 2314 12-13 -0.5156846897113141 2314 14-15 -0.6486317306371987 2314 16-17 -1.0345553963361453 2314 18-19 -0.7396817730475149 2314 20-21 -0.5408245987028408 2314 22-23 -0.4609192693913755 2314 24-25 -0.44794769859635863 2314 26-27 -0.4421489853782674 2314 28-29 -0.5165607286075868 2314 30-31 -0.5545492583685103 2314 32-33 -0.6346648720926638 2314 34-35 -0.6798296113237328 2314 36-37 -0.8143678978193307 2314 38-39 -0.7329178972368524 2314 40-41 -0.5484927672324673 2314 42-43 -0.4759494729264091 2314 44-45 -0.4882645746855516 2314 46-47 -0.41864150507582565 2314 48-49 -0.40359159101241104 2314 50-51 -0.29118725853821914 2314 52-53 -0.32128590031435067 2314 54-55 -0.3733459111167896 2314 56-57 -0.26716851988865997 2314 58-59 -0.36327627204508417 2314 60-61 -0.39555907246633737 2314 62-63 -0.3527673197655474 2314 64-65 -0.26789424693335206 2314 66-67 -0.2840753146630881 2314 68-69 -0.1803342281612892 2314 70-71 -0.2580977594470504 2314 72-73 -0.21562275037222634 2314 74-75 -0.21103869790308138 2314 76-77 -0.17248273352581478 2315 1 -1.2393636174600218 2315 2 -1.0981801283363097 2315 3 -1.1371805695007424 2315 4 -1.1178699641569878 2315 5 -0.8667311813711862 2315 6 -0.9857537996210297 2315 7 -1.1761037690338938 2315 8 -1.0839469697306612 2315 9 -1.0080303574885505 2315 10-11 -1.0177882030060843 2315 12-13 -1.1857316809150333 2315 14-15 -1.3799250567212056 2315 16-17 -1.2025332303948701 2315 18-19 -1.1598280495817832 2315 20-21 -1.1062233489328506 2315 22-23 -1.00075025547617 2315 24-25 -1.128604234691828 2315 26-27 -1.1005004189243621 2315 28-29 -0.9381317900891304 2315 30-31 -1.0135610607243564 2315 32-33 -1.1949239016253657 2315 34-35 -1.0241868526571238 2315 36-37 -1.2890417010092179 2315 38-39 -1.244319759871253 2315 40-41 -1.2435541137773392 2315 42-43 -1.355334671344501 2315 44-45 -1.2193329773082553 2315 46-47 -1.139140701084255 2315 48-49 -1.0094670865144053 2315 50-51 -0.9942969583104784 2315 52-53 -1.0276844989001752 2315 54-55 -1.135392377113817 2315 56-57 -1.220848272338781 2315 58-59 -0.883538570249673 2315 60-61 -1.0914409388733581 2315 62-63 -1.0022145119902675 2315 64-65 -1.2126742657779168 2315 66-67 -1.2248678543240494 2315 68-69 -1.3018986552098006 2315 70-71 -1.3800260400701632 2315 72-73 -2.729809687890196 2315 74-75 -3.191227171280744 2315 76-77 -2.8834945747173713 2316 1 -3.22974512486136 2316 2 -3.248632119073214 2316 3 -2.5850139698192933 2316 4 -2.738948936539934 2316 5 -3.238347098760343 2316 6 -2.7061149669075775 2316 7 -3.2914089877140498 2316 8 -3.074375526273627 2316 9 -2.7398991600267593 2316 10-11 -2.7747991896451865 2316 12-13 -2.7526443321899805 2316 14-15 -2.894988357752773 2316 16-17 -4.050536060786172 2316 18-19 -3.795450921737835 2316 20-21 -3.2326429305197877 2316 22-23 -3.245791887321154 2316 24-25 -3.363003589932525 2316 26-27 -3.1862198348557875 2316 28-29 -3.1000044947821337 2316 30-31 -3.3511300129692145 2316 32-33 -3.4917253042745813 2316 34-35 -3.512356385865843 2316 36-37 -3.3411453026874582 2316 38-39 -3.473268990391851 2316 40-41 -3.373469552913292 2316 42-43 -3.625066469972051 2316 44-45 -3.373025909623415 2316 46-47 -2.9855269814482313 2316 48-49 -3.15641192259201 2316 50-51 -3.6754800129640337 2316 52-53 -4.686843371889822 2316 54-55 -3.816041676533491 2316 56-57 -3.674766030439205 2316 58-59 -3.7351524887148386 2316 60-61 -3.5436682505545143 2316 62-63 -3.5887439971822843 2316 64-65 -3.6241814757266013 2316 66-67 -3.75475721707134 2316 68-69 -3.8916022296686563 2316 70-71 -3.61758008012945 2316 72-73 -3.605460135627908 2316 74-75 -3.658415844186603 2316 76-77 -3.630725586534414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 94642.0 3 7541.0 4 9575.0 5 11373.0 6 12756.0 7 13811.0 8 15966.0 9 16541.0 10 19705.0 11 22108.0 12 18405.0 13 16145.0 14 16248.0 15 16781.0 16 17582.0 17 18499.0 18 19531.0 19 20449.0 20 22006.0 21 23558.0 22 26269.0 23 30101.0 24 34552.0 25 40807.0 26 47988.0 27 57010.0 28 66267.0 29 79144.0 30 93953.0 31 112990.0 32 138091.0 33 172316.0 34 226797.0 35 302962.0 36 431624.0 37 592522.0 38 544847.0 39 145483.0 40 1179.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.53020385494055 26.977894353402533 28.474904885682932 22.016996905973986 2 22.306383985829044 27.032022664087098 28.426463284274472 22.23513006580939 3 22.558323481346438 27.2411232333181 27.99226344971889 22.208289835616576 4 22.144045342902327 27.149973493381214 28.342068618130977 22.36391254558548 5 22.45958145560446 27.06625130596611 28.31840472708435 22.155762511345074 6 22.509128531921824 27.069642175040144 28.29379720788206 22.12743208515597 7 22.45599250725775 26.751023730764995 28.31967609405955 22.473307667917705 8 22.28490649782952 27.240787168332016 28.108331920828917 22.365974413009546 9 22.392434286440313 26.886966015398915 28.252944534722612 22.467655163438156 10-11 22.65585206324148 27.087739626447544 28.026526025099148 22.229882285211833 12-13 22.683510743706872 26.83610530893129 28.062841191363436 22.417542755998404 14-15 22.414350877873463 27.100975614829032 28.16834506370264 22.31632844359487 16-17 22.64833633508042 26.90797846386998 28.151693907240393 22.291991293809204 18-19 22.586537741230977 26.84351983979799 28.018049710914255 22.551892708056776 20-21 22.526799479998658 27.088617949829064 27.967824460050235 22.416758110122046 22-23 22.622264094351156 27.11936541142576 27.65533397153504 22.60303652268804 24-25 22.654064685621826 27.25366502102751 27.57445362353102 22.517816669819645 26-27 22.669974630851407 27.238347009303617 27.509537852515415 22.58214050732956 28-29 22.78626509898096 27.241757395465132 27.45671831574971 22.515259189804198 30-31 22.687806804399855 27.11377252241174 27.498696542429474 22.699724130758927 32-33 22.58505711136083 27.438595238724307 27.450892212179145 22.525455437735715 34-35 22.64352815584302 27.186490052376033 27.52008722405891 22.64989456772204 36-37 22.68480137210489 27.15001677326273 27.75274397789274 22.412437876739638 38-39 22.635054560663786 27.165087134173433 27.745723962660136 22.454134342502645 40-41 22.54182528181814 27.145174621069827 27.609569709365182 22.703430387746852 42-43 22.4886911660249 27.43755159703813 27.36969718775236 22.70406004918461 44-45 22.472994965806272 27.139433955369668 27.650540943932405 22.737030134891654 46-47 22.46613985966806 27.082588382134666 27.672111838258445 22.77915991993883 48-49 22.561191069397058 27.104542409625616 27.60026379253493 22.734002728442395 50-51 22.419000637924185 27.231321880687094 27.534511585957123 22.8151658954316 52-53 22.505140236446657 27.091920895043582 27.576437206736614 22.826501661773143 54-55 22.470899803339726 27.119073853938385 27.550233691330657 22.859792651391235 56-57 22.33574875696292 27.277622296633286 27.557558632445573 22.829070313958223 58-59 22.470985514998766 27.23070045015195 27.54726426824553 22.751049766603753 60-61 22.414919695279035 27.165372867994027 27.464255330094 22.955452106632944 62-63 22.39226012158112 27.05266355252503 27.576880049510667 22.978196276383187 64-65 22.484517447564535 26.96206036719582 27.55493740916387 22.998484776075767 66-67 22.33476266473553 27.038132590189708 27.727787568724434 22.899317176350333 68-69 22.397548139454983 26.92015173846371 27.709575257138326 22.972724864942982 70-71 22.235185393739464 26.89337875786103 27.654367541343305 23.217068307056195 72-73 22.355635754007658 26.758305743628114 27.62721971719263 23.2588387851716 74-75 22.246452137915398 26.626978674220585 27.619844202501586 23.506724985362425 76-77 22.080269487186698 26.57488255647135 27.95554024023179 23.389307716110157 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5715.0 1 3536.5 2 1347.5 3 1401.0 4 1465.0 5 1552.0 6 1670.5 7 1864.5 8 2066.5 9 2106.0 10 2164.5 11 2309.0 12 2458.5 13 2522.0 14 2736.0 15 3142.5 16 3539.5 17 3744.0 18 4170.5 19 5185.5 20 6597.0 21 8730.5 22 10041.0 23 11717.5 24 15623.5 25 21625.0 26 25397.0 27 29547.0 28 39174.0 29 49176.0 30 53701.0 31 61543.5 32 76946.5 33 91187.5 34 104295.5 35 110723.0 36 118299.5 37 131726.0 38 140363.5 39 143151.0 40 147322.5 41 155654.0 42 165908.0 43 172002.0 44 172132.5 45 172374.0 46 173184.5 47 174954.0 48 176024.0 49 175590.5 50 172596.0 51 164233.5 52 158432.0 53 153996.0 54 142390.0 55 125922.5 56 108163.5 57 99702.0 58 93262.5 59 78276.5 60 63672.0 61 57614.0 62 49488.0 63 35290.0 64 24884.5 65 20551.0 66 17686.0 67 12916.0 68 9243.5 69 6256.5 70 5037.0 71 4192.5 72 2860.5 73 2066.5 74 1760.0 75 1505.5 76 1017.0 77 624.5 78 466.0 79 389.5 80 264.0 81 171.0 82 117.0 83 107.0 84 103.5 85 84.5 86 67.0 87 65.0 88 67.0 89 56.5 90 49.0 91 54.0 92 56.5 93 54.5 94 49.0 95 51.0 96 54.0 97 57.0 98 59.0 99 119.5 100 181.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008431409360663092 2 0.011944496594272712 3 0.01309678920689667 4 0.014670652287553779 5 0.011607240219846188 6 0.009077817411647262 7 0.015401374432144581 8 0.017228179793621585 9 0.01689092341919506 10-11 0.020010544882640403 12-13 0.015541897921488964 14-15 0.007447744935252396 16-17 0.005508520782299886 18-19 0.017298441538293775 20-21 0.023200428090757938 22-23 0.02058669118895238 24-25 0.01801511133395014 26-27 0.023903045537479864 28-29 0.007532059028859029 30-31 0.007841210705416674 32-33 0.009499387879680415 34-35 0.010960832168862018 36-37 0.010637628143369934 38-39 0.013448097930257632 40-41 0.010609523445501055 42-43 0.014249081819520625 44-45 0.012759532832470145 46-47 0.023720365001332164 48-49 0.022005978431330668 50-51 0.03583348978281813 52-53 0.03881258775691909 54-55 0.02738802807322061 56-57 0.019898126091164897 58-59 0.015387322083210142 60-61 0.0198278643464927 62-63 0.025589327409612478 64-65 0.02512559989477601 66-67 0.027626918005106058 68-69 0.041538743450200165 70-71 0.053272454810456296 72-73 0.08583174729155027 74-75 0.08601442782769797 76-77 0.09060954592925935 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3558124.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.2647197121504 #Duplication Level Percentage of deduplicated Percentage of total 1 74.37831944906453 39.617403381152066 2 13.486633895765815 14.367235486367061 3 4.9483054100720025 7.907103021528079 4 2.37987776522508 5.070540884555711 5 1.3104952629546942 3.490158143269131 6 0.8240263165869713 2.633491847306444 7 0.5282313635258427 1.9695266864959724 8 0.36434876095395163 1.5525547703745215 9 0.2776195448839904 1.3308594520374453 >10 1.3481523859158775 12.88524882966289 >50 0.10176821951817251 3.7512715968816064 >100 0.05047458105245095 4.627878766699037 >500 0.0016411629978584845 0.5823039337701026 >1k 5.2940741349636605E-5 0.045230774939443184 >5k 5.2940741349636605E-5 0.16919242496051065 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6000 0.16862818721326184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.431409360663092E-5 2.810469786887697E-5 3 0.0 0.0 0.0 1.967328850821388E-4 2.810469786887697E-5 4 0.0 2.810469786887697E-5 0.0 5.339892595086624E-4 2.810469786887697E-5 5 0.0 2.810469786887697E-5 0.0 0.0013209207998372177 2.810469786887697E-5 6 0.0 2.810469786887697E-5 0.0 0.0022483758295101576 2.810469786887697E-5 7 0.0 2.810469786887697E-5 0.0 0.0046372751483647005 2.810469786887697E-5 8 0.0 2.810469786887697E-5 0.0 0.007475849633121274 2.810469786887697E-5 9 0.0 2.810469786887697E-5 0.0 0.010932727470993142 2.810469786887697E-5 10 0.0 2.810469786887697E-5 0.0 0.01540137443214458 5.620939573775394E-5 11 0.0 2.810469786887697E-5 0.0 0.02093799991231334 5.620939573775394E-5 12 0.0 2.810469786887697E-5 0.0 0.026980509954121892 5.620939573775394E-5 13 0.0 2.810469786887697E-5 0.0 0.03656421192740894 5.620939573775394E-5 14 0.0 2.810469786887697E-5 0.0 0.049942048112994374 8.431409360663092E-5 15 0.0 2.810469786887697E-5 0.0 0.0626172668518579 1.1241879147550787E-4 16 0.0 2.810469786887697E-5 0.0 0.07633235941186985 1.4052348934438485E-4 17 0.0 2.810469786887697E-5 0.0 0.08911999694220887 1.4052348934438485E-4 18 0.0 2.810469786887697E-5 0.0 0.10213247205549891 1.4052348934438485E-4 19 0.0 2.810469786887697E-5 0.0 0.11669070555157718 1.6862818721326183E-4 20 0.0 2.810469786887697E-5 0.0 0.13147377663060647 1.6862818721326183E-4 21 0.0 2.810469786887697E-5 0.0 0.14887058461144131 1.967328850821388E-4 22 0.0 2.810469786887697E-5 0.0 0.16848766372391744 1.967328850821388E-4 23 0.0 2.810469786887697E-5 0.0 0.1885263133044267 1.967328850821388E-4 24 0.0 2.810469786887697E-5 0.0 0.20974536019542883 2.2483758295101575E-4 25 0.0 2.810469786887697E-5 0.0 0.23425265673708953 2.2483758295101575E-4 26 0.0 2.810469786887697E-5 0.0 0.2594906754233411 2.529422808198927E-4 27 0.0 2.810469786887697E-5 0.0 0.28158096794827836 2.529422808198927E-4 28 0.0 2.810469786887697E-5 0.0 0.30676277723879214 2.810469786887697E-4 29 0.0 2.810469786887697E-5 0.0 0.33163543485274827 3.3725637442652367E-4 30 0.0 2.810469786887697E-5 0.0 0.36041464547047825 3.3725637442652367E-4 31 0.0 2.810469786887697E-5 0.0 0.3906834050752588 3.934657701642776E-4 32 0.0 2.810469786887697E-5 0.0 0.4237907391647958 3.934657701642776E-4 33 0.0 2.810469786887697E-5 0.0 0.460523579279418 4.2157046803315457E-4 34 0.0 2.810469786887697E-5 0.0 0.49857734019387745 4.2157046803315457E-4 35 0.0 2.810469786887697E-5 0.0 0.5383735923762072 4.2157046803315457E-4 36 0.0 2.810469786887697E-5 0.0 0.5810084190432936 4.2157046803315457E-4 37 0.0 2.810469786887697E-5 0.0 0.6251608993952993 4.2157046803315457E-4 38 0.0 2.810469786887697E-5 0.0 0.6697068455174693 4.2157046803315457E-4 39 0.0 2.810469786887697E-5 0.0 0.7170913661243958 4.2157046803315457E-4 40 0.0 2.810469786887697E-5 0.0 0.7667804719565704 4.496751659020315E-4 41 0.0 2.810469786887697E-5 0.0 0.820319921396781 4.496751659020315E-4 42 0.0 2.810469786887697E-5 0.0 0.8764450030409283 4.496751659020315E-4 43 0.0 2.810469786887697E-5 0.0 0.939343316871475 4.496751659020315E-4 44 0.0 2.810469786887697E-5 0.0 1.0074691045056328 4.496751659020315E-4 45 0.0 2.810469786887697E-5 0.0 1.0792485028627445 4.496751659020315E-4 46 0.0 2.810469786887697E-5 0.0 1.1551592918065812 4.496751659020315E-4 47 0.0 2.810469786887697E-5 0.0 1.2300021022314005 4.496751659020315E-4 48 0.0 2.810469786887697E-5 0.0 1.3102129099491755 4.777798637709085E-4 49 0.0 2.810469786887697E-5 0.0 1.395510667981217 4.777798637709085E-4 50 0.0 2.810469786887697E-5 0.0 1.484349617944737 4.777798637709085E-4 51 0.0 2.810469786887697E-5 0.0 1.5776010054736709 4.777798637709085E-4 52 0.0 2.810469786887697E-5 0.0 1.6779628815634307 4.777798637709085E-4 53 0.0 2.810469786887697E-5 0.0 1.7846483146736876 4.777798637709085E-4 54 0.0 2.810469786887697E-5 0.0 1.8993997960723124 4.777798637709085E-4 55 0.0 2.810469786887697E-5 0.0 2.018929076108646 4.777798637709085E-4 56 0.0 2.810469786887697E-5 0.0 2.1468897655056427 4.777798637709085E-4 57 0.0 2.810469786887697E-5 0.0 2.272461555583785 4.777798637709085E-4 58 0.0 2.810469786887697E-5 0.0 2.4069987442820993 4.777798637709085E-4 59 0.0 2.810469786887697E-5 0.0 2.5446555544438585 4.777798637709085E-4 60 0.0 2.810469786887697E-5 0.0 2.687848990085787 4.777798637709085E-4 61 0.0 2.810469786887697E-5 0.0 2.8355110726888664 4.777798637709085E-4 62 0.0 2.810469786887697E-5 0.0 2.990002596874083 4.777798637709085E-4 63 0.0 2.810469786887697E-5 0.0 3.1560170471855393 5.058845616397854E-4 64 0.0 2.810469786887697E-5 0.0 3.330772058534216 5.058845616397854E-4 65 0.0 2.810469786887697E-5 0.0 3.5159820174901157 5.339892595086624E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCGTA 445 0.0 19.143698 36 GCGTAAG 475 0.0 18.6819 38 TAACGCG 140 1.6732662E-4 17.752544 52 TGCGTAA 490 0.0 17.386826 37 CGGTATA 125 0.0016055161 17.037413 10 CGTAAGT 560 0.0 16.480335 39 GCGTTCG 130 0.0020870022 16.382128 10 CGGTTAC 260 2.3333996E-8 16.380285 4 CGAACTC 785 0.0 15.374542 25 CGCGGAA 215 1.7305569E-5 14.859672 59 CGCTAAA 215 1.731707E-5 14.858625 1 TCGAACT 800 0.0 14.643585 24 ACGCGAA 180 0.0011319522 13.805982 54 CGTTCGG 180 0.0011335156 13.80346 11 ATAACGC 210 2.3508612E-4 13.525939 51 CTCGCGC 265 8.322942E-6 13.394569 36 TTAGCGG 320 2.9604416E-7 13.311601 42 CTTGCGT 700 0.0 13.184638 35 TCGCGGA 270 1.0088032E-5 13.147076 58 AACGCGA 190 0.0017014662 13.0795355 53 >>END_MODULE