FastQCFastQC Report
Thu 26 May 2016
SRR936099_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936099_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3558124
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77590.21806435076461642No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57160.16064645301850075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40340.1133743512030497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12600.047.4739100-101
CGGTATC10200.036.47029100-101
CGTACAT21250.035.431618100-101
GGTATCA87600.034.3043331
TACGCAG11100.032.17271100-101
GCGTATC4450.032.10041100-101
GTATCAA167200.030.8563481
CGAGTAC11850.028.127365100-101
CCGTACA10600.028.075243100-101
CCTGTCG9150.027.64276396-97
TCGTATC4800.026.65978100-101
ACGTATC5150.026.003672100-101
TCGTACA11100.025.470062100-101
CCGTATC7400.024.129534100-101
ATCAACG211750.023.968373
TATCAAC214100.023.7337442
TCGAGTA6650.023.718304100-101
TCAACGC214400.023.6992024
CAACGCA221050.022.9580365
AGCGTAC3900.022.12905198-99
Produced by FastQC (version 0.11.5)