FastQCFastQC Report
Thu 26 May 2016
SRR936098_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936098_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566216
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60360.16925503110299545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC3250.022.9342654
CGCGATA952.5269217E-422.42292620
TACGCGA952.5271619E-422.42261157
CGATCGT2757.2759576E-1219.3595521
TTACGCG1309.49991E-519.11349756
TTGCGTA4750.018.68053236
CGCGGAA2901.4551915E-1118.36463459
CCTCGCG2901.4551915E-1118.35716635
CGCTCCG3002.3646862E-1117.74725225
TGCGTAA5000.017.74675237
GCGTAAG5100.017.39999838
CGTAAGT4900.017.38701239
CTCGCGC3155.0931703E-1116.90143236
GTTACGC1502.838961E-416.56549555
GCGTTCG1502.8404797E-416.56433310
ATTCGCG3451.0913936E-1116.46214356
TCGCGCT3301.0186341E-1016.13341137
TCGCGGA3351.2732926E-1015.89662758
CGTTCGG1858.0209655E-515.34940411
AATTCGC3805.2750693E-1114.94631255
Produced by FastQC (version 0.11.5)