FastQCFastQC Report
Thu 26 May 2016
SRR936098_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936098_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566216
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79570.22312165051135432No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56950.15969307523717016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41040.1150799615054164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13500.046.958168100-101
GGTATCA91650.037.5315321
CGTACAT20450.036.82074100-101
TACGCAG11350.036.448887100-101
CGGTATC11250.034.921005100-101
GCGTATC4550.030.743393100-101
TCGTATC5900.030.266665100-101
GTATCAA170650.030.1655141
CCGTACA10150.029.908834100-101
GCGTACA7700.028.989174100-101
CCTGTCG9850.028.39714696-97
CGAGTAC12250.027.940044100-101
ACGTATC5450.027.850885100-101
CCGGTAT4550.026.164589100-101
GACGCAG13350.023.40849100-101
TATCAAC218650.023.347222
ATCAACG217700.023.2571413
TCGGTAT5900.023.204443100-101
AGTATCA38200.023.139736100-101
TCAACGC220200.022.9930954
Produced by FastQC (version 0.11.5)