FastQCFastQC Report
Thu 26 May 2016
SRR936097_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936097_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966908
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84170.42793053869321795No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26280.1336107230231409No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA1151.2587407E-930.86254510
CGCGCTT1154.282265E-827.77911638
GCGGTAT1652.2682798E-923.6612859
CGGTTAC1853.4015102E-1023.0200354
TCGCGGA1252.7365713E-622.71887658
CGCTCCG1303.8461058E-621.84507425
CGCGGAA1355.3339572E-621.03659
ATTCGCG1407.3001484E-620.28677656
CTCGCGC1501.3267752E-518.93047136
CGATCGT1501.327648E-518.9290281
CAATTCG1803.1970194E-617.75047754
TTAGTCG1401.674603E-417.75002515
TCGCGCT1602.3105287E-517.74912537
TAAGGCG7700.017.52084417
GTTCGCG2055.7564284E-717.31665824
AATTCGC1653.0076137E-517.21258455
CGAACTC5200.017.06646325
CGTGTCG1250.001596440617.05129871
ATCGTTC1905.368456E-616.8106843
GCCTCGA5350.016.58965321
Produced by FastQC (version 0.11.5)