FastQCFastQC Report
Thu 26 May 2016
SRR936097_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936097_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966908
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91780.46662070620486573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68480.34816066638602317No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50650.2575107732542651No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12500.046.905125100-101
GGTATCA83450.040.855561
TACGCAG7950.040.057213100-101
CGTACAT15250.038.641983100-101
CGGTATC10650.038.285564100-101
TAAATCG1950.038.1497996-97
TCGAGTA3250.036.63032100-101
AGTATCA28200.036.30558100-101
CCGTATC5500.034.632305100-101
AGGTATC19050.034.5272100-101
CGAGTAC9900.034.27155100-101
CCGTACA7250.033.662003100-101
GTATCAA166450.031.9220351
GCGTACA4300.031.838564100-101
GGGTATC13050.031.472603100-101
CCTGTCG6150.030.96646796-97
TCCCGTA2500.030.95262198-99
ACGTATC3850.030.9217100-101
TCGTACA6950.030.404484100-101
TGTATCA24000.030.382181100-101
Produced by FastQC (version 0.11.5)