FastQCFastQC Report
Thu 26 May 2016
SRR936096_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936096_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968024
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83690.42524887907871045No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27710.14080112844152307No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGA750.001687203223.66918452
GTTCGCG1159.168669E-418.52135524
ATTCGCG1200.001219387517.75098656
CGGTATA1200.001221051317.74737510
AGTCGTA1604.6329972E-415.53290264
TTGCGCG2754.642243E-815.488629
TAAGGCG8250.015.48861817
CGGGGTG5600.015.21667849
TCGCGGA1400.003414525215.21590358
CGATTCG1400.003420886615.211655
TAACGGA1500.005395946614.20331662
TATACGG1759.1549585E-414.19862312
ATACGTA2001.557883E-414.197541
CTCGAAC5600.013.94613823
TCGAACG2051.9216229E-413.85090529
GCCTCGA5650.013.822721521
CGCTCCG1550.00671796613.74130125
TTCGCGC1550.00671796613.74130125
CGATCGT1550.006723620513.7395541
TCGAACT5500.013.55426424
Produced by FastQC (version 0.11.5)