FastQCFastQC Report
Thu 26 May 2016
SRR936096_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936096_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968024
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89630.455431437827994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68610.348623797270765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49880.2534521936724349No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14150.048.373295100-101
CGGTATC11350.041.690346100-101
GGTATCA85000.041.6588331
TACGCAG9100.039.898144100-101
AGTATCA26450.039.717625100-101
CGTACAT17350.037.39308100-101
ACGTATC4700.037.358414100-101
CGAGTAC8400.035.42868100-101
AGGTATC19600.035.378067100-101
TCGGTAT3400.035.01187100-101
CCGTACA6650.034.906574100-101
TCGAGTA3550.034.370808100-101
CCGTATC6450.034.143364100-101
TCGTATC5050.033.5906100-101
GCGTATC3200.032.5501100-101
GGGTATC11950.031.876917100-101
GTATCAA171500.031.4046171
CCCGGTA3800.031.3240298-99
CGGGTAT2300.031.05401100-101
ACCGTAT3000.030.7497598-99
Produced by FastQC (version 0.11.5)