FastQCFastQC Report
Thu 26 May 2016
SRR936095_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936095_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826759
Sequences flagged as poor quality0
Sequence length77
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100340.3549648201350027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31730.1122486918764564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA1806.188202E-921.69300710
CGCGGAA2452.5465852E-1120.28508859
CGGTTAC3300.019.3606414
CGTAAGT4750.018.68363239
TCGCGGA3051.8189894E-1218.62204658
TGCGTAA4800.018.48868437
TCGCGCT2801.70985E-1017.74913837
GCGTAAG5000.017.74882338
CGATCGT2801.70985E-1017.7466261
GCCGTAA2902.8012437E-1017.13740239
TAACGCG1250.001603346417.0406852
TTGCGTA5250.016.90184836
CTCGCGC3155.0931703E-1116.90184636
ATTCGCG2953.583409E-1016.84663856
CGTAAAC1703.8923496E-516.7032998
AACTCGA3451.0913936E-1116.46209313
TATCGGC1956.807528E-616.39658471
ATTGCGC1553.6413898E-416.03289845
TATAGCG1553.6428618E-416.03204743
CGCTCCG3107.239578E-1016.03034625
Produced by FastQC (version 0.11.5)