FastQCFastQC Report
Thu 26 May 2016
SRR936095_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936095_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2826759
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106560.3769688183534571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75880.2684346277839745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55250.19545352115266987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13950.047.789196100-101
CGGTATC11100.041.290836100-101
CGTACAT22550.037.614407100-101
CGAGTAC12450.036.574463100-101
TACGCAG12350.036.38865100-101
GGTATCA106900.036.1268351
TCGTATC5700.033.416504100-101
CCGTACA8650.033.030186100-101
CCTGTCG8900.032.0981196-97
GTATCAA214800.031.2491171
ACGTATC5400.030.863855100-101
ACCGTAT3300.030.66289598-99
CCGTATC7000.030.186739100-101
ACGGTAT4550.030.088625100-101
GCGTACA6850.029.97881100-101
TCGGTAT5200.029.761574100-101
GCGTATC4050.029.394148100-101
AGTATCA40900.029.106674100-101
TCGTACA11750.028.368479100-101
CCGGTAT3900.028.23534100-101
Produced by FastQC (version 0.11.5)