FastQCFastQC Report
Thu 26 May 2016
SRR936094_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936094_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2845014
Sequences flagged as poor quality0
Sequence length77
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99840.3509297318044832No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31950.11230173208286497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT1951.8189894E-1225.4806421
TCGCGCT2250.023.66309237
CGCGGAA2055.0931703E-1122.5139459
CGGTTAC2750.021.940214
CAGCGTA2601.8189894E-1220.48019612
CGCGCTT2601.8189894E-1220.47947738
ATTCGCG2503.45608E-1119.87769756
CGCTCCG2554.5474735E-1119.48725325
CGGTATA1651.3942918E-619.36071210
TCGCGGA2507.185008E-1018.46110558
AATTCGC3350.018.01315355
CAACGCG1200.001221259617.7473210
CTCGCGC3053.092282E-1117.45515336
GACGCTC3305.456968E-1217.21133623
TACGTAG3551.8189894E-1216.995642
CGTAAGT4550.016.38444539
CAATTCG3258.0035534E-1116.38329354
CCTCGCG3258.0035534E-1116.38041335
ATACGTA3751.8189894E-1216.0892051
GACGTAT3551.8189894E-1115.99983439
Produced by FastQC (version 0.11.5)