FastQCFastQC Report
Thu 26 May 2016
SRR936094_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936094_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2845014
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107580.3781352218301913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73330.2577491710058369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55770.19602715487516054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14250.048.87118100-101
CGGTATC11600.044.898506100-101
CGTACAT21650.039.040222100-101
TACGCAG11100.038.87737100-101
CGAGTAC10750.037.37465100-101
TCGTATC5500.037.3369100-101
CCGTACA9650.036.083645100-101
GGTATCA107400.035.9624861
CCGGTAT5550.034.319332100-101
ACGTATC5650.034.23866100-101
ACCGTAC3150.032.12217398-99
ACGGTAT3650.031.799742100-101
GCGTACA6550.031.351595100-101
AGTATCA40300.031.164433100-101
GTATCAA211300.031.122331
TCGGTAT3850.030.920828100-101
CCGTATC6150.030.003258100-101
CCCGGTA3900.029.76024898-99
GACGCAG10900.028.123058100-101
TCGTACA10550.027.645563100-101
Produced by FastQC (version 0.11.5)