FastQCFastQC Report
Thu 26 May 2016
SRR936093_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936093_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466712
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41250.11898882860762591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG2350.022.65812356
CGGTTAC2705.456968E-1219.7181154
GACGTAT4400.018.55589939
TCGCGGA2601.2132659E-917.74886358
CGGTATA1502.8390996E-416.56536710
CAATTCG3856.548362E-1114.753365554
AATTCGC3856.548362E-1114.752089555
CGCGGAA2908.897223E-814.6893559
CGCTCCG3705.711627E-1014.39034725
AATCGAG5450.014.32935857
GACGCGA3756.89397E-1014.20934269
GTACGTA1759.1541494E-414.19929640
TCGCGGT3504.0290615E-914.19786233
AAATCGA6400.013.31164756
CGTTCGG1600.00829121813.31145611
CGCGCTT3557.40456E-812.998980538
CGTATTA5850.012.74240741
ATCGAGG6700.012.71560458
CGAACTC9500.012.703925
GTAGACC20450.012.4972568
Produced by FastQC (version 0.11.5)