FastQCFastQC Report
Thu 26 May 2016
SRR936093_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936093_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3466712
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51610.14887305319853508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36240.10453709451491788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11500.045.8045100-101
GGTATCA74750.037.097931
CGTACAT21400.033.51475100-101
CGGTATC11200.032.151413100-101
GCGTATC3600.031.41332100-101
GCGCGTA1101.5825208E-1029.75955698-99
GTATCAA142350.028.3848931
ACGTATC5250.028.342842100-101
CCTGTCG9200.028.1395596-97
AACGTAT4650.027.51958898-99
TCGTACA10800.026.45332100-101
TCGTATC6650.025.956078100-101
CCGGTAT5500.025.43126100-101
CCGTACA9250.025.416636100-101
TACGCAG9750.025.028912100-101
CGAGTAC13800.025.015638100-101
GCGTACA8350.024.235678100-101
TCGAGTA5100.023.924694100-101
GGGTATC15600.022.892296100-101
CCGTATC5700.022.450516100-101
Produced by FastQC (version 0.11.5)