FastQCFastQC Report
Thu 26 May 2016
SRR936092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480670
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42220.1212984856363861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC3200.019.9653434
GTTCGCG2206.202026E-817.74824724
TTCGCGC2206.204027E-817.74773625
ATTCGCG3751.8189894E-1216.0933656
TCGAACT10450.015.62525124
CGCTCCG4150.015.39562825
TCGCGCT3754.3655746E-1115.14473437
CGATCGT4251.8189894E-1215.0325131
CGAACTC10950.014.91134125
GCCTCGA10600.014.39972921
CAATTCG5450.014.32988954
CGTTCGC2757.8244193E-714.19900623
CGATTCG2252.6609363E-514.1975775
CGTCGTG3504.0308805E-914.1975774
AATCGAG5550.014.07351357
CTCGAAC11050.013.81351223
CGCGGAA3605.9699232E-913.80637559
GACGTAT4657.2759576E-1213.74136739
TCGCGCA2851.171602E-613.69961326
GTAGACC21550.013.6707178
Produced by FastQC (version 0.11.5)