FastQCFastQC Report
Thu 26 May 2016
SRR936092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3480670
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48530.13942717924997197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36030.10351455323256731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12750.043.8809100-101
GGTATCA75400.035.5980031
CGGTATC11450.034.567986100-101
CGTACAT19500.034.032833100-101
CCGTACG2450.030.36350396-97
GTATCAA143150.028.9775621
CGAGTAC12650.028.230501100-101
TCGTATC6500.027.47045100-101
GCGTATC4700.027.226917100-101
CCTGTCG8600.026.64220896-97
TCGTACA10700.025.865866100-101
ACGTATC5700.025.58286100-101
CGTACGT1900.025.05787896-97
GCGTACA7450.024.766422100-101
AGGTATC23850.024.331793100-101
CCGTACA10900.024.026142100-101
CGTATAG5750.023.807722100-101
AACGTAT4750.023.80635598-99
AGCGTAC3900.023.6537598-99
GACGCAG11950.023.409266100-101
Produced by FastQC (version 0.11.5)