FastQCFastQC Report
Thu 26 May 2016
SRR936091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574706
Sequences flagged as poor quality0
Sequence length77
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147890.9391594367456528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72620.46116544929656705No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC802.5465852E-1144.363114
TCGCTAA905.5371947E-627.60809757
CGGTATA1259.605537E-825.55720910
CGCCGTA700.001125294725.37291367
TCGCGCT700.00112968325.35598637
CGTTAGG952.5315254E-422.41575629
CTCGCTA1355.329737E-621.03741356
CGCTAAT1205.1320472E-520.70607258
TACGTAG1407.3168594E-620.2809222
GCCGTAT1256.983167E-519.8936368
CGTTTAG1106.7696546E-419.3756867
GCGGTAT1701.8542596E-618.7914689
ACGAGTA950.006624844418.69404866
ATTTGCG3450.018.5167773
ATACGTA1752.4478595E-618.253411
CGGCGGA1000.00893085217.75031920
TACGCCT1000.00893575617.74862740
TACGAGC1000.008937390517.74806438
TTGCGCG3450.017.4886235
CGTTACG1300.002087483216.38074530
Produced by FastQC (version 0.11.5)