##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936091_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1574706 Sequences flagged as poor quality 0 Sequence length 77 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.70937432130188 40.0 36.0 41.0 30.0 41.0 2 36.55581994353231 39.0 36.0 41.0 30.0 41.0 3 36.467729849254404 39.0 36.0 41.0 30.0 41.0 4 36.33892929854843 39.0 36.0 41.0 30.0 41.0 5 36.25266875213532 39.0 35.0 41.0 30.0 41.0 6 36.114582658604206 39.0 35.0 40.0 29.0 41.0 7 35.898257198486576 39.0 35.0 40.0 29.0 41.0 8 35.662016274783994 38.0 35.0 40.0 28.0 41.0 9 35.53869484208481 38.0 35.0 40.0 27.0 41.0 10-11 35.68663293338566 39.0 35.0 40.0 28.5 41.0 12-13 35.76454049200295 39.0 35.0 41.0 28.5 41.0 14-15 35.653665509625284 39.0 35.0 41.0 27.5 41.0 16-17 35.42813801433411 39.0 35.0 41.0 26.5 41.0 18-19 35.18717589188077 39.0 35.0 41.0 25.5 41.0 20-21 34.83464595930923 39.0 34.5 41.0 24.0 41.0 22-23 34.384457797201506 39.0 34.0 41.0 21.5 41.0 24-25 34.02139129462897 38.5 33.5 41.0 16.5 41.0 26-27 33.06093740672862 37.5 32.0 39.5 13.5 40.5 28-29 33.079080475974564 38.0 33.0 39.5 11.0 40.5 30-31 33.43869744574543 38.0 33.0 40.0 8.5 41.0 32-33 33.291753190754335 38.0 33.0 40.0 7.5 41.0 34-35 33.029164809177075 38.0 33.0 40.0 4.5 41.0 36-37 32.75081570782102 37.5 32.0 40.0 2.0 41.0 38-39 32.49290121457592 37.0 32.0 40.0 2.0 41.0 40-41 32.127109441381435 36.5 31.5 40.0 2.0 41.0 42-43 31.769835448648827 36.0 31.0 39.0 2.0 41.0 44-45 31.39893954808072 35.5 31.0 39.0 2.0 41.0 46-47 31.010688344363963 35.0 31.0 39.0 2.0 40.0 48-49 30.553896092349937 35.0 30.0 38.0 2.0 40.0 50-51 30.10354536021327 35.0 30.0 37.0 2.0 39.5 52-53 29.64041382962915 35.0 29.0 37.0 2.0 39.0 54-55 29.1689347090822 34.5 29.0 36.0 2.0 39.0 56-57 28.698591991139935 34.0 29.0 36.0 2.0 37.5 58-59 28.239981304446673 34.0 27.5 35.0 2.0 37.0 60-61 27.857982061413367 34.0 27.0 35.0 2.0 36.5 62-63 27.488360366951035 34.0 26.0 35.0 2.0 36.0 64-65 27.087402664370366 34.0 26.0 35.0 2.0 36.0 66-67 26.672918309830536 33.5 25.0 35.0 2.0 35.5 68-69 26.28744254483059 33.0 24.5 35.0 2.0 35.0 70-71 25.84668439696045 33.0 23.5 35.0 2.0 35.0 72-73 25.349124534992562 33.0 19.0 35.0 2.0 35.0 74-75 24.7647783141742 33.0 7.5 35.0 2.0 35.0 76-77 23.138547131972572 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.049156621687679 1101 2 0.8573278098172707 1101 3 1.0377873881344115 1101 4 0.994539541818753 1101 5 0.9909271735348923 1101 6 1.0275456496741668 1101 7 1.1168002351572 1101 8 1.113111097296681 1101 9 1.279184028122529 1101 10-11 1.183377663886489 1101 12-13 1.1436579495504233 1101 14-15 1.2948463996002317 1101 16-17 1.2754624571408186 1101 18-19 1.3395508363697672 1101 20-21 1.2826695959312175 1101 22-23 1.3003356755288848 1101 24-25 1.399246234694047 1101 26-27 1.2673748448425357 1101 28-29 1.3143340124354168 1101 30-31 1.480487063277188 1101 32-33 1.4091864321325005 1101 34-35 1.1783889461052226 1101 36-37 1.357253998782788 1101 38-39 1.4275002334124665 1101 40-41 1.27600114314383 1101 42-43 1.3081892229739616 1101 44-45 1.2336478098945314 1101 46-47 1.163447572513423 1101 48-49 1.2075075429214266 1101 50-51 1.096355009196607 1101 52-53 1.2237724419635967 1101 54-55 1.0818921544460913 1101 56-57 1.165042869285596 1101 58-59 1.18094089684611 1101 60-61 1.343839432928302 1101 62-63 1.1444053458717143 1101 64-65 1.1241366272109126 1101 66-67 1.2413717678564922 1101 68-69 1.4135819698000667 1101 70-71 1.3279634890201493 1101 72-73 1.409909426996844 1101 74-75 1.5076484080851174 1101 76-77 1.342979089384034 1102 1 1.0118106056253993 1102 2 1.13864039569971 1102 3 0.9801771463136433 1102 4 0.8979885402431549 1102 5 1.0066670822224708 1102 6 0.910627665639737 1102 7 0.8537017781195715 1102 8 1.0252702372029532 1102 9 1.0488347359348609 1102 10-11 0.8813036911512668 1102 12-13 1.206421137667725 1102 14-15 1.175813340150441 1102 16-17 1.1135053046700278 1102 18-19 1.1234686068314446 1102 20-21 0.9472641703774372 1102 22-23 0.9422497306306568 1102 24-25 0.9473368741630992 1102 26-27 0.870006625273632 1102 28-29 1.0501697064864075 1102 30-31 1.1301824198832335 1102 32-33 1.259143516098824 1102 34-35 1.2499358172644435 1102 36-37 1.2536243701978904 1102 38-39 1.1968401525771881 1102 40-41 1.2139779040108607 1102 42-43 1.1823550803476586 1102 44-45 0.9372204185849249 1102 46-47 0.8707461190098265 1102 48-49 0.9527293563555546 1102 50-51 1.141083388985372 1102 52-53 1.0811758607394601 1102 54-55 1.2418903813893003 1102 56-57 1.3840702871910544 1102 58-59 1.273626433135341 1102 60-61 1.2349712274632552 1102 62-63 1.3442021495338814 1102 64-65 1.3260298690437686 1102 66-67 1.3089368168774804 1102 68-69 1.3524019400610676 1102 70-71 1.304476027352429 1102 72-73 1.2302065953445513 1102 74-75 1.1727270471834395 1102 76-77 1.5118088517541288 1103 1 0.6907650637638767 1103 2 0.6231206182051992 1103 3 0.6187355787839053 1103 4 0.8630267908076448 1103 5 0.8399088943813666 1103 6 0.5291751750413098 1103 7 0.6746045865971482 1103 8 0.9288657307928361 1103 9 0.751446261259133 1103 10-11 0.7689673141606832 1103 12-13 0.8182319287080304 1103 14-15 0.8681240251319196 1103 16-17 0.8738155614513516 1103 18-19 0.9054493100708925 1103 20-21 0.7877866241229015 1103 22-23 0.857928497255628 1103 24-25 0.6684078404086407 1103 26-27 0.4679311431024189 1103 28-29 0.5470142083766305 1103 30-31 0.6218454025748272 1103 32-33 0.7212002248652354 1103 34-35 0.7906534886485801 1103 36-37 0.7000956342011264 1103 38-39 0.7069011215386283 1103 40-41 0.7002177615153613 1103 42-43 0.7217546981393745 1103 44-45 0.8099510569005197 1103 46-47 0.3563245956084877 1103 48-49 0.49539226569805095 1103 50-51 0.4965881398264109 1103 52-53 0.5597338868160584 1103 54-55 0.7103155762104585 1103 56-57 0.6517625361735817 1103 58-59 0.6298626925691266 1103 60-61 0.5579047652489457 1103 62-63 0.5983476126938001 1103 64-65 0.4622151112048307 1103 66-67 0.6592330382093827 1103 68-69 0.5715732637347877 1103 70-71 0.6562491427265975 1103 72-73 0.46618081016226043 1103 74-75 0.6065047042545899 1103 76-77 0.6359479910321717 1104 1 0.3449402595765818 1104 2 0.5903948849896068 1104 3 0.3498930160295117 1104 4 0.4592876907774297 1104 5 0.4587391150310438 1104 6 0.4976007249924024 1104 7 0.3641650088377659 1104 8 0.5988863462298255 1104 9 0.44869564500256587 1104 10-11 0.5582272097886616 1104 12-13 0.6424451983376755 1104 14-15 0.4899534499704359 1104 16-17 0.575267322735165 1104 18-19 0.6252403720592952 1104 20-21 0.5125816942643695 1104 22-23 0.4403400523753689 1104 24-25 0.36869037519082326 1104 26-27 0.2227218922948495 1104 28-29 0.3832875813889913 1104 30-31 0.5533889124948317 1104 32-33 0.45113121091938524 1104 34-35 0.5438191536406976 1104 36-37 0.4697480718031741 1104 38-39 0.4159380639555579 1104 40-41 0.5369341409189303 1104 42-43 0.5211045674682531 1104 44-45 0.3035770508764024 1104 46-47 0.27081708777767943 1104 48-49 0.2665129045583683 1104 50-51 0.1695577666098913 1104 52-53 0.17661192111886592 1104 54-55 0.29925729286386016 1104 56-57 0.37650838338268855 1104 58-59 0.44096205160410307 1104 60-61 0.1219971452965396 1104 62-63 0.10867933646149197 1104 64-65 0.14327621213996977 1104 66-67 0.20422328333958717 1104 68-69 0.06733693934451068 1104 70-71 0.0356813283169366 1104 72-73 0.036856211838362185 1104 74-75 0.0025512056194969546 1104 76-77 0.20314796271080127 1105 1 0.1162684919703878 1105 2 0.3027257530581835 1105 3 0.3954776927617303 1105 4 0.5153568431580595 1105 5 0.4887681983388177 1105 6 0.1433112211127323 1105 7 0.3573841106634248 1105 8 0.49314125687818233 1105 9 0.29777628301763315 1105 10-11 0.2848519271920722 1105 12-13 0.43715226353654657 1105 14-15 0.04879318503967767 1105 16-17 0.16427814945090802 1105 18-19 0.3020656052369901 1105 20-21 0.22422666205687847 1105 22-23 0.28521653352234466 1105 24-25 0.17798382690230596 1105 26-27 0.2308461893535707 1105 28-29 0.3587329195392428 1105 30-31 0.44479158565980015 1105 32-33 0.49799396270017127 1105 34-35 0.620260822295478 1105 36-37 0.5360049024042723 1105 38-39 0.5181038331295227 1105 40-41 0.855753511992404 1105 42-43 0.5879805544262062 1105 44-45 0.2722159293513471 1105 46-47 0.3052874818792226 1105 48-49 0.42662020605911266 1105 50-51 0.5653982003601641 1105 52-53 0.39862562776371746 1105 54-55 0.36356505901588676 1105 56-57 0.501503413090834 1105 58-59 0.4956693440163136 1105 60-61 0.18561191075556494 1105 62-63 0.1905755454068796 1105 64-65 0.6110729811662452 1105 66-67 0.530341172982066 1105 68-69 0.23207016247937062 1105 70-71 0.12640051534759777 1105 72-73 -0.027014878672073195 1105 74-75 0.00847721359468423 1105 76-77 0.33005584474163285 1106 1 0.13189109915899877 1106 2 0.37238387750491597 1106 3 0.09603497269778671 1106 4 -0.0014516646987701165 1106 5 -0.029486267931183363 1106 6 0.11343913030449215 1106 7 0.1969761048067511 1106 8 0.2431197799369471 1106 9 0.1466444482145306 1106 10-11 -0.12627392208088395 1106 12-13 -0.07359741244177798 1106 14-15 -0.17180394862906923 1106 16-17 0.025637957316320126 1106 18-19 0.026989642229622746 1106 20-21 -0.06550427855844987 1106 22-23 0.13603980070669763 1106 24-25 0.14024954148683832 1106 26-27 0.12653595400364281 1106 28-29 0.16464159958510294 1106 30-31 0.25305269110597806 1106 32-33 0.023215361950910562 1106 34-35 0.032954721723633895 1106 36-37 0.2054509654008072 1106 38-39 0.17369003223384283 1106 40-41 0.13196168331489844 1106 42-43 0.09855888176467076 1106 44-45 -0.11958324070494086 1106 46-47 0.09472078010242413 1106 48-49 -0.07126524900926157 1106 50-51 -0.04480255301358582 1106 52-53 -0.024375569342307557 1106 54-55 0.16508847448451647 1106 56-57 -0.027005125393976925 1106 58-59 -0.09125063850332182 1106 60-61 0.05856389765276404 1106 62-63 -0.04983640100161324 1106 64-65 0.08052483359911733 1106 66-67 0.019955204334667798 1106 68-69 -0.15608236302216127 1106 70-71 -0.3168149189161511 1106 72-73 0.0033586520250175056 1106 74-75 -0.24141866992932393 1106 76-77 -0.1876549280920905 1107 1 0.08297482019178659 1107 2 0.05095420361344338 1107 3 -0.02820995046054975 1107 4 0.12297803124196349 1107 5 -0.07010893897830073 1107 6 0.007470396152399417 1107 7 -0.012800095224115182 1107 8 0.07327169932485589 1107 9 0.05977963221558724 1107 10-11 0.20584038127280735 1107 12-13 0.20715402627231327 1107 14-15 0.2640126456425378 1107 16-17 0.29649214557288417 1107 18-19 0.24174787211518378 1107 20-21 -0.01521366933744872 1107 22-23 -0.032449163639654444 1107 24-25 -0.17998241670777304 1107 26-27 -0.2884407840291203 1107 28-29 -0.10663372950006789 1107 30-31 -0.003141165970731663 1107 32-33 0.15263524597180833 1107 34-35 0.11489241052040455 1107 36-37 0.1860921578246817 1107 38-39 0.09517196286687835 1107 40-41 0.10545693169979131 1107 42-43 0.18446830341263265 1107 44-45 0.16536946363655858 1107 46-47 0.19566250391341455 1107 48-49 0.22207322915095062 1107 50-51 0.15490683776541658 1107 52-53 0.28582860678716315 1107 54-55 0.09093726067286312 1107 56-57 0.024011345401778073 1107 58-59 -7.364910901230814E-5 1107 60-61 0.21524217692866898 1107 62-63 0.18034991522884525 1107 64-65 0.1220451299411458 1107 66-67 0.05727136864573623 1107 68-69 0.07373110025480045 1107 70-71 -0.015870769686774366 1107 72-73 0.2700580985886383 1107 74-75 0.2726064221263229 1107 76-77 0.19704553273740189 1108 1 -0.15654607447704905 1108 2 -0.15077413392070582 1108 3 -0.16788588717289343 1108 4 -0.17252236059926673 1108 5 -0.3239890143812616 1108 6 -0.3494863742286398 1108 7 -0.33865144972393324 1108 8 -0.332791884857464 1108 9 -0.2864347578888342 1108 10-11 -0.3693503500935549 1108 12-13 -0.21657079528107914 1108 14-15 0.011224430532294605 1108 16-17 0.030663877934742345 1108 18-19 -0.017545754640153177 1108 20-21 -0.02744398725836561 1108 22-23 -0.02043806500157075 1108 24-25 -0.07342108826010474 1108 26-27 -0.16984548188776216 1108 28-29 -0.1305116868423184 1108 30-31 -0.1853291226775724 1108 32-33 -0.08410421138103885 1108 34-35 -0.0038359058331423057 1108 36-37 0.03825321516058722 1108 38-39 -0.1580182601649156 1108 40-41 -0.04047808619782245 1108 42-43 0.008801643806169324 1108 44-45 -0.14903885610588574 1108 46-47 -0.2616947917466774 1108 48-49 -0.16367603482295578 1108 50-51 -0.21877879966495328 1108 52-53 -0.23781932916267934 1108 54-55 -0.1804905240474497 1108 56-57 -0.0912270423304129 1108 58-59 -0.14210338668129197 1108 60-61 -0.07643447992431973 1108 62-63 0.1401080326964923 1108 64-65 0.003603810812936814 1108 66-67 -0.09475032029586927 1108 68-69 -0.2326957313381932 1108 70-71 -0.18349663544408656 1108 72-73 -0.027508673275043805 1108 74-75 -0.13711505289114712 1108 76-77 -0.02237962558763229 1109 1 0.18306436670267345 1109 2 0.18667686527687977 1109 3 -0.1212769184583351 1109 4 -0.023009645452489735 1109 5 0.17555466462914637 1109 6 0.10791173252746944 1109 7 -0.04369406789110997 1109 8 0.297033932017861 1109 9 0.21726593373148262 1109 10-11 -0.12137493544467048 1109 12-13 -0.04691117211584839 1109 14-15 0.14657687394350205 1109 16-17 0.3051898834574587 1109 18-19 0.048510126128789466 1109 20-21 0.08274744189210992 1109 22-23 0.17353936587871033 1109 24-25 0.10994530646558331 1109 26-27 0.023766111052218264 1109 28-29 0.012285102984002094 1109 30-31 0.1980540667435875 1109 32-33 0.19360106905348573 1109 34-35 0.19508728078456983 1109 36-37 0.15723430225041568 1109 38-39 0.2025004919588369 1109 40-41 0.12440177531320273 1109 42-43 0.2506847501218026 1109 44-45 0.24803027424568924 1109 46-47 0.0015169268714352313 1109 48-49 0.07329212772886606 1109 50-51 0.11956965251074081 1109 52-53 0.17270812408883174 1109 54-55 0.2863614339666789 1109 56-57 0.27030156137943706 1109 58-59 0.20818275240048223 1109 60-61 0.17284979264621114 1109 62-63 0.10162062285948892 1109 64-65 0.0449163748508532 1109 66-67 0.15726710452617354 1109 68-69 0.1705963327559168 1109 70-71 0.18541230389060814 1109 72-73 0.020316717440312715 1109 74-75 -0.1740831286486788 1109 76-77 -0.07501211815912612 1110 1 -0.2930338669707382 1110 2 -0.13397874982256752 1110 3 -0.14057640526140602 1110 4 -0.12202346310938594 1110 5 0.015620603351692353 1110 6 -0.047581507651223376 1110 7 -0.1423555755086383 1110 8 -0.12815570508647056 1110 9 -0.08862858472438262 1110 10-11 -0.22228012322020874 1110 12-13 -0.22890686356410583 1110 14-15 -0.21883558803416747 1110 16-17 -0.06071062794322302 1110 18-19 0.08563617433280513 1110 20-21 -0.15419412322176385 1110 22-23 -0.502886111974945 1110 24-25 -0.31101232365949727 1110 26-27 -0.29360685627602834 1110 28-29 -0.31290502400683096 1110 30-31 -0.27406547209358934 1110 32-33 -0.0874480392102086 1110 34-35 0.03654735483090832 1110 36-37 -0.026031215822925446 1110 38-39 -0.028841466370607804 1110 40-41 -0.027721454002261225 1110 42-43 -0.02029269999448502 1110 44-45 -0.11732156427792617 1110 46-47 -0.2857204684510428 1110 48-49 0.03470866719514021 1110 50-51 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-1.2448032973354515 2314 18-19 -0.7604777636588409 2314 20-21 -0.580359598676921 2314 22-23 -0.4234561614208445 2314 24-25 -0.6159629594625073 2314 26-27 -0.576507006934051 2314 28-29 -0.6060363579349541 2314 30-31 -0.6068141477082278 2314 32-33 -0.8514422416540626 2314 34-35 -0.825517215695676 2314 36-37 -0.9119979599428518 2314 38-39 -0.8933943453768549 2314 40-41 -0.6558487518639566 2314 42-43 -0.46615466979098485 2314 44-45 -0.4896194423200875 2314 46-47 -0.58986062290003 2314 48-49 -0.5997632617178006 2314 50-51 -0.5257144286623046 2314 52-53 -0.49506745056050505 2314 54-55 -0.49111769751113243 2314 56-57 -0.36828256140825744 2314 58-59 -0.5015073408652881 2314 60-61 -0.5772719039725125 2314 62-63 -0.4632018854197284 2314 64-65 -0.44090247203871513 2314 66-67 -0.4445634154471101 2314 68-69 -0.3449649229573559 2314 70-71 -0.21502762239201445 2314 72-73 -0.1425908176086672 2314 74-75 -0.39860709553880724 2314 76-77 -0.23315757359473466 2315 1 -1.3724015238942329 2315 2 -1.292594183483331 2315 3 -1.3219733913207676 2315 4 -1.1862692805859893 2315 5 -1.0979584013438952 2315 6 -1.118336055460503 2315 7 -1.212477514578957 2315 8 -1.2205992684170752 2315 9 -1.096887034451079 2315 10-11 -1.2807725219530042 2315 12-13 -1.3605341457706999 2315 14-15 -1.4815787522069286 2315 16-17 -1.3674863490507647 2315 18-19 -1.1214779343364256 2315 20-21 -1.3195685080471264 2315 22-23 -1.2590083034246007 2315 24-25 -1.4632619866002443 2315 26-27 -1.507096698007615 2315 28-29 -1.5413111488549056 2315 30-31 -1.6844852519922355 2315 32-33 -1.7861281948884056 2315 34-35 -1.5974194174365834 2315 36-37 -1.766004616368864 2315 38-39 -1.6324624457352748 2315 40-41 -1.5599194123862326 2315 42-43 -1.72843492239382 2315 44-45 -1.6204907514172042 2315 46-47 -1.7125095390973364 2315 48-49 -1.6140020396662536 2315 50-51 -1.4781039149227553 2315 52-53 -1.671859565255847 2315 54-55 -1.5947616640583213 2315 56-57 -1.6394295148132727 2315 58-59 -1.4680844017971886 2315 60-61 -1.5547040596304704 2315 62-63 -1.8251877191797554 2315 64-65 -1.8338150851126187 2315 66-67 -1.8722985238273786 2315 68-69 -1.8150323406054163 2315 70-71 -1.845655715923332 2315 72-73 -3.1573074928092133 2315 74-75 -3.8024473413145365 2315 76-77 -3.4029007038217323 2316 1 -3.2074350492629478 2316 2 -3.2108385145600877 2316 3 -2.5799551447266467 2316 4 -2.610272410051465 2316 5 -3.531045062967202 2316 6 -3.072641333523862 2316 7 -3.266966099142415 2316 8 -3.419965939609831 2316 9 -3.2403662370839683 2316 10-11 -2.994494807755963 2316 12-13 -2.9939190441600587 2316 14-15 -3.0847078943842305 2316 16-17 -4.276016728789418 2316 18-19 -3.9289682840735907 2316 20-21 -3.5984451010679024 2316 22-23 -3.409692389058158 2316 24-25 -3.4327517996728787 2316 26-27 -3.0222692712170627 2316 28-29 -3.1946858312206246 2316 30-31 -3.418836469832776 2316 32-33 -3.4252667189962835 2316 34-35 -3.567630818001696 2316 36-37 -3.3942527299607015 2316 38-39 -3.3402240373658927 2316 40-41 -3.5503910305914452 2316 42-43 -3.673440062734823 2316 44-45 -3.545141272887019 2316 46-47 -3.2125971511987963 2316 48-49 -3.532563358438523 2316 50-51 -3.7728358736189875 2316 52-53 -4.8209777866332 2316 54-55 -4.1019326014108195 2316 56-57 -4.025922703053208 2316 58-59 -3.9832104209748636 2316 60-61 -3.931118461637471 2316 62-63 -3.952050527034455 2316 64-65 -3.7586227264396648 2316 66-67 -3.8339163410898784 2316 68-69 -3.8400514148267675 2316 70-71 -3.5612652181184856 2316 72-73 -3.7205558327053687 2316 74-75 -3.358667800518333 2316 76-77 -3.5504244496108583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37190.0 3 4065.0 4 6303.0 5 7647.0 6 8589.0 7 8825.0 8 11197.0 9 12417.0 10 17301.0 11 20433.0 12 13745.0 13 9230.0 14 8669.0 15 8656.0 16 8490.0 17 8996.0 18 9415.0 19 9745.0 20 10343.0 21 11089.0 22 12289.0 23 13884.0 24 15944.0 25 18472.0 26 21331.0 27 24977.0 28 29231.0 29 34344.0 30 40526.0 31 48396.0 32 58885.0 33 73139.0 34 97095.0 35 128299.0 36 173881.0 37 243640.0 38 237192.0 39 70173.0 40 663.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.642822076304636 27.09257270457659 32.25694592193233 20.007659297186446 2 20.365472896930342 27.317041259872926 32.337466688006664 19.980019155190064 3 20.579666698634668 27.64626790499279 31.569948637369155 20.204116759003384 4 20.131930612576724 27.376935896914762 32.188224055932686 20.30290943457583 5 20.393554361260115 27.621970350366652 31.923783853131297 20.060691435241935 6 20.501181918989328 27.49323937257313 32.077700535642386 19.92787817279516 7 20.59516581007233 27.15452741652334 31.96680495816957 20.28350181523476 8 20.133558513783623 27.624852249147473 31.810083705899427 20.431505531169474 9 20.36106668631379 27.39535049153798 31.812142175724034 20.431440646424203 10-11 20.592197613043783 27.543429518734243 31.706152859202614 20.158220009019367 12-13 20.791609768490584 27.20092730794881 31.40915875385055 20.598304169710058 14-15 20.397362853990998 27.72636021900429 31.544614090986745 20.331662836017962 16-17 20.635968011075775 27.613770415802037 31.471842983092614 20.278418590029577 18-19 20.53301431699105 27.62405655913309 31.132770545145743 20.710158578730113 20-21 20.592065104231178 27.855598896551374 30.884620001943674 20.667715997273774 22-23 20.80746347626092 27.952942954940557 30.168059060447018 21.071534508351505 24-25 20.833979080337702 28.29943902581548 29.741190952258762 21.125390941588055 26-27 20.953083896244216 28.452235123288837 29.444338755846527 21.15034222462042 28-29 21.004708599067932 28.58038341993921 29.335988456501667 21.078919524491194 30-31 20.83576919669381 28.467671793067467 29.337533021098256 21.359025989140466 32-33 20.950615053236234 28.81453835520694 29.023774267825143 21.211072323731685 34-35 21.012514000329624 28.328759971711953 29.383305922802332 21.27542010515609 36-37 21.038777763558308 28.546128400115972 29.391527023890085 21.023566812435636 38-39 21.016965838090172 28.382378392969144 29.54116815789265 21.059487611048038 40-41 20.856024060612626 28.396464073098944 29.43411133590575 21.313400530382683 42-43 20.88809511258067 28.699702947847243 29.054552290896435 21.35764964867565 44-45 21.036589722638848 28.381781004058748 29.29496008096541 21.286669192336994 46-47 20.76906073558023 28.422605152987046 29.430482059568746 21.377852051863975 48-49 20.872017299843524 28.437745240176977 29.15972596936068 21.530511490618824 50-51 20.635252384467513 28.632541073064377 29.03510856045119 21.697097982016917 52-53 20.477360916642738 28.583970949601422 29.31286735464001 21.625800779115835 54-55 20.46435386456552 28.426802062185725 29.182589572698653 21.9262545005501 56-57 20.32316141524603 28.66175871380766 29.041621688743565 21.973458182202748 58-59 20.464052478541493 28.59818194983086 28.9411305904217 21.996634981205947 60-61 20.40684561106873 28.31138079029155 28.95788884647363 22.32388475216609 62-63 20.350706246359515 28.279549948580616 29.056875111556877 22.312868693502992 64-65 20.459165135819784 28.061249120239445 29.101081629075164 22.378504114865603 66-67 20.246155390235156 28.185811715360238 29.19356175752319 22.374471136881414 68-69 20.36446260856196 28.04160113745268 29.19900244180037 22.394933812184988 70-71 20.168618516966532 27.79791066293865 29.23356345925291 22.79990736084191 72-73 20.40621045452783 27.5678855331977 29.16244795331227 22.8634560589622 74-75 20.192197074618022 27.447304543920453 29.10579006478603 23.25470831667549 76-77 20.083304359654147 27.302930780344344 29.53875597721175 23.07500888278976 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 16910.0 1 10064.5 2 2980.5 3 2792.0 4 2842.0 5 2775.5 6 2815.5 7 2967.5 8 3112.5 9 3212.0 10 3329.5 11 3406.5 12 3636.5 13 3907.0 14 3991.0 15 4345.5 16 4896.5 17 5177.0 18 5603.5 19 6556.0 20 7650.0 21 9351.5 22 10485.0 23 11370.0 24 13543.5 25 16972.0 26 19112.0 27 21310.0 28 25879.5 29 30104.0 30 31957.0 31 35593.0 32 42969.5 33 50071.5 34 55117.0 35 56801.0 36 60233.0 37 65584.0 38 67270.0 39 67037.0 40 66952.5 41 68033.0 42 70236.0 43 71274.0 44 70053.5 45 68290.5 46 67464.5 47 67609.5 48 68038.0 49 66484.5 50 63856.0 51 59329.5 52 55878.0 53 53538.0 54 48237.0 55 42226.5 56 35934.0 57 32691.0 58 31502.0 59 27334.0 60 22389.0 61 20423.0 62 17940.5 63 12562.0 64 8223.0 65 6780.0 66 5811.5 67 4237.5 68 3166.0 69 2334.0 70 1968.0 71 1623.5 72 1139.5 73 926.5 74 853.0 75 783.5 76 532.5 77 294.5 78 238.0 79 194.0 80 142.0 81 108.0 82 72.0 83 62.0 84 56.0 85 50.5 86 39.5 87 28.0 88 28.0 89 24.5 90 19.5 91 18.0 92 18.5 93 19.0 94 16.5 95 17.0 96 20.0 97 18.5 98 17.5 99 41.5 100 65.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009462083715944437 2 0.012573775676221467 3 0.013653342274684926 4 0.015367948048715125 5 0.01168472083042803 6 0.009398579798387763 7 0.016129995059395212 8 0.01682853815251863 9 0.016511018564735257 10-11 0.020067237947909006 12-13 0.016257002894508564 14-15 0.00793798969458426 16-17 0.006255135879332395 18-19 0.017971608668538763 20-21 0.02343294557841273 22-23 0.02025774970057903 24-25 0.01917818310211557 26-27 0.02378221712497444 28-29 0.008890548457934369 30-31 0.009176316086939402 32-33 0.010636906190742906 34-35 0.012033992376989737 36-37 0.011589464954093019 38-39 0.013875605986133285 40-41 0.011589464954093019 42-43 0.014923420625818407 44-45 0.013113558975453197 46-47 0.022670898567732644 48-49 0.022639146608954306 50-51 0.035562193831737476 52-53 0.03899140537979788 54-55 0.02883077857073003 56-57 0.020480013412027386 58-59 0.017273065575415348 60-61 0.020638773205919073 62-63 0.025211055269999606 64-65 0.026290621868463065 66-67 0.028989538364621713 68-69 0.042071345381296574 70-71 0.054454609304848016 72-73 0.08630182395952007 74-75 0.0871908788053135 76-77 0.09138213736405398 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1574706.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.70144365301876 #Duplication Level Percentage of deduplicated Percentage of total 1 77.5940496147013 43.99694632037527 2 12.184563886371137 13.817647252793606 3 4.374709845968973 7.4415709148854825 4 2.0478297128982454 4.644596043075098 5 1.056780746643996 2.9960496979714812 6 0.617996040180539 2.102476059005133 7 0.4172336507393658 1.6560425236279022 8 0.2921285249546074 1.3251287277722523 9 0.20215131479763415 1.031604424684354 >10 1.0712019112033138 11.266350260575056 >50 0.09304785122002869 3.613006701683102 >100 0.04763289051314752 4.550333173883744 >500 4.493398717489538E-4 0.15369735864528628 >1k 0.0 0.0 >5k 1.1233496793723844E-4 0.4625570735524529 >10k+ 1.1233496793723844E-4 0.9419934674700118 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14789 0.9391594367456528 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7262 0.46116544929656705 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.350391755667406E-5 0.0 2 0.0 0.0 0.0 5.715352580100666E-4 0.0 3 0.0 0.0 0.0 0.0013335822686901555 0.0 4 0.0 0.0 0.0 0.0031751958778337037 0.0 5 0.0 0.0 0.0 0.007048934848790822 0.0 6 0.0 0.0 0.0 0.011748224747984704 0.0 7 0.0 0.0 0.0 0.021908851557052553 0.0 8 0.0 0.0 0.0 0.03194247053100706 0.0 9 0.0 0.0 0.0 0.04299215218586835 0.0 10 0.0 0.0 0.0 0.05740754147123336 0.0 11 0.0 0.0 0.0 0.07429958354130867 0.0 12 1.2700783511334813E-4 0.0 0.0 0.09716099386171133 0.0 13 1.2700783511334813E-4 0.0 0.0 0.12738885861868818 0.0 14 1.2700783511334813E-4 0.0 0.0 0.16479266605956921 0.0 15 1.2700783511334813E-4 0.0 0.0 0.20264100092334697 0.0 16 1.2700783511334813E-4 0.0 0.0 0.24607768053211201 0.0 17 1.2700783511334813E-4 0.0 0.0 0.2865931799332701 0.0 18 1.2700783511334813E-4 0.0 0.0 0.3276167106748815 0.0 19 1.2700783511334813E-4 0.0 0.0 0.37333953131568687 0.0 20 1.2700783511334813E-4 0.0 0.0 0.4254127437121596 0.0 21 1.2700783511334813E-4 0.0 0.0 0.47818449920175576 0.0 22 1.905117526700222E-4 0.0 0.0 0.5340044427340722 0.0 23 2.5401567022669626E-4 0.0 0.0 0.5976353681258597 0.0 24 3.1751958778337036E-4 0.0 0.0 0.6604407425894103 0.0 25 3.1751958778337036E-4 0.0 0.0 0.7306125714895352 0.0 26 3.1751958778337036E-4 0.0 0.0 0.8005303847194334 0.0 27 3.1751958778337036E-4 0.0 0.0 0.8682890647524045 0.0 28 3.1751958778337036E-4 0.0 0.0 0.9338886115884489 0.0 29 3.1751958778337036E-4 0.0 0.0 0.9992341427542665 0.0 30 3.810235053400444E-4 0.0 0.0 1.072962191037565 0.0 31 3.810235053400444E-4 0.0 0.0 1.1532946467467577 0.0 32 4.445274228967185E-4 0.0 0.0 1.2397234785413913 0.0 33 4.445274228967185E-4 0.0 0.0 1.3320581746687954 0.0 34 4.445274228967185E-4 0.0 0.0 1.4329658996663504 0.0 35 4.445274228967185E-4 0.0 0.0 1.5385094106455428 0.0 36 4.445274228967185E-4 0.0 0.0 1.6547215797742563 0.0 37 4.445274228967185E-4 0.0 0.0 1.7676950491075794 0.0 38 4.445274228967185E-4 0.0 0.0 1.8798429675126658 0.0 39 4.445274228967185E-4 0.0 0.0 1.9905302958139488 0.0 40 4.445274228967185E-4 0.0 0.0 2.1086475824693625 0.0 41 4.445274228967185E-4 0.0 0.0 2.234194827478907 0.0 42 4.445274228967185E-4 0.0 0.0 2.3717443129066633 0.0 43 4.445274228967185E-4 0.0 0.0 2.5207245034946206 0.0 44 4.445274228967185E-4 0.0 0.0 2.679801816974089 0.0 45 4.445274228967185E-4 0.0 0.0 2.843895939940535 0.0 46 4.445274228967185E-4 0.0 0.0 3.0224689561099023 0.0 47 5.080313404533925E-4 0.0 0.0 3.199073350835013 0.0 48 5.080313404533925E-4 0.0 0.0 3.3824726647386876 0.0 49 5.080313404533925E-4 0.0 0.0 3.5646654042087857 0.0 50 5.080313404533925E-4 0.0 0.0 3.748382237700244 0.0 51 5.080313404533925E-4 0.0 0.0 3.94683198006485 0.0 52 5.080313404533925E-4 0.0 0.0 4.152648176866031 0.0 53 5.715352580100666E-4 0.0 0.0 4.371546180683886 0.0 54 5.715352580100666E-4 0.0 0.0 4.609050832345847 0.0 55 5.715352580100666E-4 0.0 0.0 4.8464284761726955 0.0 56 7.620470106800888E-4 0.0 0.0 5.106223002897049 0.0 57 7.620470106800888E-4 0.0 0.0 5.367351111890092 0.0 58 7.620470106800888E-4 0.0 0.0 5.635591659649484 0.0 59 7.620470106800888E-4 0.0 0.0 5.905483309265349 0.0 60 7.620470106800888E-4 0.0 0.0 6.165912875165269 0.0 61 7.620470106800888E-4 0.0 0.0 6.445838143755088 0.0 62 7.620470106800888E-4 0.0 0.0 6.733256874616595 0.0 63 7.620470106800888E-4 0.0 0.0 7.031725287132963 0.0 64 7.620470106800888E-4 0.0 0.0 7.3451806241927065 0.0 65 7.620470106800888E-4 0.0 0.0 7.66244619630585 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 80 2.5465852E-11 44.36311 4 TCGCTAA 90 5.5371947E-6 27.608097 57 CGGTATA 125 9.605537E-8 25.557209 10 CGCCGTA 70 0.0011252947 25.372913 67 TCGCGCT 70 0.001129683 25.355986 37 CGTTAGG 95 2.5315254E-4 22.415756 29 CTCGCTA 135 5.329737E-6 21.037413 56 CGCTAAT 120 5.1320472E-5 20.706072 58 TACGTAG 140 7.3168594E-6 20.280922 2 GCCGTAT 125 6.983167E-5 19.89363 68 CGTTTAG 110 6.7696546E-4 19.37568 67 GCGGTAT 170 1.8542596E-6 18.791468 9 ACGAGTA 95 0.0066248444 18.694048 66 ATTTGCG 345 0.0 18.516777 3 ATACGTA 175 2.4478595E-6 18.25341 1 CGGCGGA 100 0.008930852 17.750319 20 TACGCCT 100 0.008935756 17.748627 40 TACGAGC 100 0.0089373905 17.748064 38 TTGCGCG 345 0.0 17.488623 5 CGTTACG 130 0.0020874832 16.380745 30 >>END_MODULE