FastQCFastQC Report
Thu 26 May 2016
SRR936091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1574706
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135580.860986114233387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101190.642596141755985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71530.4542435222828896No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21740.13805751676820943No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.13278669161100548No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA10850.052.945816100-101
CGGTATC9450.045.98595100-101
ACGTATC3300.043.294365100-101
CGAGTAC8650.042.668728100-101
GGTATCA93050.042.5251851
TCGGTAT3450.042.274754100-101
AGGTATC19200.041.23676100-101
AACGTAT1950.041.2122598-99
TACGCAG8650.040.60411100-101
CCTGTCG5900.040.3476296-97
GACGCAG5800.039.002254100-101
AGTATCA28800.038.75635100-101
CGTACAT13700.038.23809100-101
TCGAGTA3100.037.446136100-101
CACGCAG8300.037.29575100-101
GCGTATC2350.036.73113100-101
TCGTATC4400.036.529625100-101
CCGTACA5050.035.953613100-101
GCGTACA2650.035.942493100-101
CCGTATC4800.035.345795100-101
Produced by FastQC (version 0.11.5)