FastQCFastQC Report
Thu 26 May 2016
SRR936090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575272
Sequences flagged as poor quality0
Sequence length77
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147610.9370445231045812No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73160.4644277305760529No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG552.7534197E-432.27567352
CGCACGA500.0067371628.3926724
CGGTATA1800.027.60486210
AACGCGA657.324972E-427.30931953
ACGCGAA750.001688450123.66582154
CGGTTAC1652.2664608E-923.660564
GCGGTAT2159.458745E-1121.4602589
TAATCGA1205.121626E-520.71153846
TCGCTAA1257.011717E-519.88307857
CGTTTAG1106.7706703E-419.37524867
CGCGAAT1106.8011374E-419.3623355
TCGATTA2404.1472958E-1019.23214359
TAGAGCG2852.1827873E-1017.4390845
TATCGAT2651.5570549E-917.4177957
CGATTAT2902.8012437E-1017.14058560
CCGTCAC4500.016.5771368
ATGTTCG1350.00268252315.77671255
GTAGCCG5900.015.64350823
TTTATCG2754.6355126E-815.48986355
CCTACGA1857.99502E-515.35421561
Produced by FastQC (version 0.11.5)