##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936090_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1575272 Sequences flagged as poor quality 0 Sequence length 77 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.705190595655864 40.0 36.0 41.0 30.0 41.0 2 36.556085552209396 39.0 36.0 41.0 30.0 41.0 3 36.414351299331166 39.0 36.0 41.0 30.0 41.0 4 36.306992062323204 39.0 36.0 41.0 30.0 41.0 5 36.223725807352636 39.0 35.0 41.0 29.0 41.0 6 36.09017680756085 39.0 35.0 40.0 29.0 41.0 7 35.889861560416236 39.0 35.0 40.0 29.0 41.0 8 35.712517584264816 38.0 35.0 40.0 28.0 41.0 9 35.81236129379561 39.0 35.0 40.0 29.0 41.0 10-11 35.948588561213555 39.0 35.0 41.0 29.5 41.0 12-13 35.78802422692716 39.0 35.0 41.0 28.0 41.0 14-15 35.59049421306289 39.0 35.0 41.0 27.0 41.0 16-17 35.383342368809956 39.0 35.0 41.0 26.0 41.0 18-19 35.143536798724284 39.0 35.0 41.0 25.5 41.0 20-21 34.79131381755024 39.0 34.5 41.0 24.0 41.0 22-23 34.32912823944056 38.5 34.0 41.0 21.0 41.0 24-25 33.96846322412891 38.5 33.0 40.5 16.5 41.0 26-27 32.98309244371765 37.5 32.0 39.5 12.0 40.5 28-29 32.982247827676744 38.0 32.5 39.5 9.0 40.5 30-31 33.381682655439825 38.0 33.0 40.0 8.5 41.0 32-33 33.26353099655171 38.0 33.0 40.0 7.5 41.0 34-35 33.02550543652144 38.0 33.0 40.0 4.0 41.0 36-37 32.73176727574666 37.5 32.0 40.0 2.0 41.0 38-39 32.443152357180224 37.0 32.0 40.0 2.0 41.0 40-41 32.098557899842056 36.5 31.5 40.0 2.0 41.0 42-43 31.732430653245913 36.0 31.0 39.0 2.0 41.0 44-45 31.368634750062213 35.5 31.0 39.0 2.0 41.0 46-47 30.960436356387977 35.0 31.0 39.0 2.0 40.0 48-49 30.540130529838656 35.0 30.0 38.0 2.0 40.0 50-51 30.075891655536314 35.0 30.0 37.0 2.0 39.5 52-53 29.586360006398895 35.0 29.0 37.0 2.0 39.0 54-55 29.104494652352102 34.0 29.0 36.0 2.0 39.0 56-57 28.63462087817215 34.0 28.5 36.0 2.0 37.5 58-59 28.18139502257388 34.0 27.5 35.0 2.0 37.0 60-61 27.796670035397064 34.0 27.0 35.0 2.0 36.5 62-63 27.3983458094856 34.0 26.0 35.0 2.0 36.0 64-65 26.98801699008171 34.0 26.0 35.0 2.0 36.0 66-67 26.599941470425424 33.5 25.0 35.0 2.0 35.5 68-69 26.20986375686231 33.0 24.5 35.0 2.0 35.0 70-71 25.738616886480557 33.0 21.5 35.0 2.0 35.0 72-73 25.245686459227358 33.0 19.0 35.0 2.0 35.0 74-75 24.673880447313223 32.5 4.5 35.0 2.0 35.0 76-77 22.99206137098863 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.9480813106226762 1101 2 0.9520783478560588 1101 3 1.0586838859281187 1101 4 0.8466634163686138 1101 5 0.8204283998460298 1101 6 0.7417327243314986 1101 7 0.9718888259647969 1101 8 0.9743991176171392 1101 9 0.9479766920020083 1101 10-11 0.8810590923823725 1101 12-13 1.0717633919348302 1101 14-15 1.2288424061905872 1101 16-17 1.046615711076278 1101 18-19 1.2593626976218744 1101 20-21 1.1464260910932893 1101 22-23 1.1774093256861917 1101 24-25 1.200311398522011 1101 26-27 1.252600044893235 1101 28-29 1.31302413788233 1101 30-31 1.281293641974493 1101 32-33 1.1536866042884455 1101 34-35 1.2411363673446019 1101 36-37 1.405417804714645 1101 38-39 1.2511711574336246 1101 40-41 1.1697224854564183 1101 42-43 1.314229795758056 1101 44-45 1.167782181472262 1101 46-47 1.1771543208472792 1101 48-49 1.2349905557578644 1101 50-51 1.2187798799808007 1101 52-53 1.2451045724989065 1101 54-55 1.1806306186369042 1101 56-57 1.1652195063338304 1101 58-59 1.121733262641687 1101 60-61 1.3121130621792574 1101 62-63 1.2356535160587399 1101 64-65 1.269320156773393 1101 66-67 1.4415335021192526 1101 68-69 1.4595872032002148 1101 70-71 1.5371804353585077 1101 72-73 1.3692999044714007 1101 74-75 1.381545834789133 1101 76-77 1.672889435827333 1102 1 0.7109684673301047 1102 2 0.8573035274495737 1102 3 0.8970768231556434 1102 4 0.7574515941101936 1102 5 0.8231480238759943 1102 6 0.9442535807780388 1102 7 0.7348378577162862 1102 8 0.8623793866728562 1102 9 0.7762935900765484 1102 10-11 1.0170570816832623 1102 12-13 1.196019032756432 1102 14-15 1.0934185597404635 1102 16-17 1.2198010815135376 1102 18-19 1.080001574239681 1102 20-21 1.2075713453973194 1102 22-23 1.2997964840723952 1102 24-25 1.3467010376657953 1102 26-27 1.2296657480359627 1102 28-29 1.3117796820541585 1102 30-31 1.4461719280069971 1102 32-33 1.4026681842317217 1102 34-35 1.4796577072798627 1102 36-37 1.3641752099343094 1102 38-39 1.4929123979179408 1102 40-41 1.4784746384921164 1102 42-43 1.462902815888544 1102 44-45 1.2823301554962612 1102 46-47 1.3609047351462635 1102 48-49 1.345647836603895 1102 50-51 1.3649800567803432 1102 52-53 1.2961310986660557 1102 54-55 1.4752534374816406 1102 56-57 1.4851995816103383 1102 58-59 1.4918990349981271 1102 60-61 1.3186775726685163 1102 62-63 1.2850537838002154 1102 64-65 1.248063920704272 1102 66-67 1.2689004503893138 1102 68-69 1.2935112273612184 1102 70-71 1.1432990138441212 1102 72-73 1.2391591795165233 1102 74-75 1.3634380311949137 1102 76-77 1.1640118059580864 1103 1 0.4905104169411487 1103 2 0.4542373617755189 1103 3 0.638131236519996 1103 4 0.6114622331029835 1103 5 0.6245430781359929 1103 6 0.5471345723117196 1103 7 0.46877073493049437 1103 8 0.33377679821101935 1103 9 0.46280967494992353 1103 10-11 0.5567601782379512 1103 12-13 0.6661050308081329 1103 14-15 0.525396174118157 1103 16-17 0.4975239508038811 1103 18-19 0.6178081979073156 1103 20-21 0.6394460063610765 1103 22-23 0.8385403388186106 1103 24-25 0.8531189404834478 1103 26-27 0.7916096220948177 1103 28-29 0.8670464947016612 1103 30-31 1.074838844601949 1103 32-33 0.9829718471011901 1103 34-35 0.8806818452402823 1103 36-37 0.8422546530486414 1103 38-39 0.8979858001519503 1103 40-41 0.8463080158469012 1103 42-43 0.9941076406405394 1103 44-45 1.017687339940494 1103 46-47 0.9458718326552145 1103 48-49 0.8147895033415615 1103 50-51 0.9252596356511802 1103 52-53 1.0132264775488196 1103 54-55 1.0756947881168308 1103 56-57 1.0350190936253547 1103 58-59 1.0122106785322238 1103 60-61 0.7674507939885338 1103 62-63 0.6420749226211768 1103 64-65 0.7436092329868238 1103 66-67 0.7869158777808885 1103 68-69 0.5883378538121526 1103 70-71 0.37211546139752727 1103 72-73 0.4939830794770437 1103 74-75 0.5655086018550364 1103 76-77 0.5918151919183678 1104 1 0.5824120219885742 1104 2 0.4925506073006005 1104 3 0.3940354138613955 1104 4 0.5110166010596586 1104 5 0.5855924083697701 1104 6 0.5610392712676386 1104 7 0.34104908877533546 1104 8 0.3027186080627544 1104 9 0.2629004396424577 1104 10-11 0.49376298497600146 1104 12-13 0.5048025668856795 1104 14-15 0.6697009721243461 1104 16-17 0.7202232212026303 1104 18-19 0.737780243511871 1104 20-21 0.7306970055730062 1104 22-23 0.6237497896451458 1104 24-25 0.612471752447064 1104 26-27 0.4994607679990608 1104 28-29 0.7025976312846396 1104 30-31 0.8224633329913189 1104 32-33 0.8349825580881358 1104 34-35 0.7429412458355245 1104 36-37 0.7167995349650909 1104 38-39 0.7950730914943804 1104 40-41 0.8006168436547938 1104 42-43 0.6486084018776133 1104 44-45 0.6694818727800893 1104 46-47 0.522152777624882 1104 48-49 0.6685418324822088 1104 50-51 0.6195153792955317 1104 52-53 0.6390918727565555 1104 54-55 0.6231814817681034 1104 56-57 0.7482214960650815 1104 58-59 0.6228967616873717 1104 60-61 0.6344974671895045 1104 62-63 0.43855863817366725 1104 64-65 0.5809902573285228 1104 66-67 0.46665845065862754 1104 68-69 0.3754012017299786 1104 70-71 0.415141597674495 1104 72-73 0.1492883887407892 1104 74-75 -0.12424303807653203 1104 76-77 0.05684912981611134 1105 1 -0.03413453795133847 1105 2 0.19121965262817753 1105 3 0.158060906220733 1105 4 0.20973846204883273 1105 5 -0.17267030846786469 1105 6 -0.09190901633124327 1105 7 0.2340621635857829 1105 8 0.11290498866414822 1105 9 0.16926414002507784 1105 10-11 0.303670616252262 1105 12-13 0.5197376623059782 1105 14-15 0.42537865574679756 1105 16-17 0.4823297445236818 1105 18-19 0.4022423453404613 1105 20-21 0.3626745413116055 1105 22-23 0.43575537636846207 1105 24-25 0.4258710857960466 1105 26-27 0.3627855725811244 1105 28-29 0.5160439118883815 1105 30-31 0.6772965528687891 1105 32-33 0.8185568873292368 1105 34-35 0.7748653790718336 1105 36-37 0.6935635780626797 1105 38-39 0.6651671033720348 1105 40-41 0.6127720747526553 1105 42-43 0.8008231386208742 1105 44-45 0.5111610587146913 1105 46-47 0.4617874106147184 1105 48-49 0.3548960260457399 1105 50-51 0.44520902498459947 1105 52-53 0.3908377237929166 1105 54-55 0.11284428311079608 1105 56-57 0.2736463687206516 1105 58-59 0.31442689008811087 1105 60-61 0.09310172530551242 1105 62-63 0.15424606335147928 1105 64-65 0.11458688651563165 1105 66-67 -0.02090346103358698 1105 68-69 -0.11274461644330458 1105 70-71 -0.0128823923726884 1105 72-73 -0.5052414345027962 1105 74-75 -0.6546707275673889 1105 76-77 -0.5891656350955117 1106 1 0.3244578581583042 1106 2 0.42021523176168785 1106 3 0.24943137483258226 1106 4 0.5868259952053378 1106 5 0.470664793212066 1106 6 0.38319266359802384 1106 7 0.07237516093326235 1106 8 0.15100224772692883 1106 9 0.3108096749499225 1106 10-11 0.10367857381194767 1106 12-13 0.5300621539063357 1106 14-15 0.43510986706421306 1106 16-17 0.4423164819242089 1106 18-19 0.4687763860124434 1106 20-21 0.5531873618244205 1106 22-23 0.4158888865364574 1106 24-25 0.1113485393769551 1106 26-27 0.19594295542815843 1106 28-29 0.33272560950111085 1106 30-31 0.657347834920067 1106 32-33 0.4787284169490391 1106 34-35 0.4252641412292135 1106 36-37 0.368650227045876 1106 38-39 0.4168364225585961 1106 40-41 0.44400372815672995 1106 42-43 0.2887577097968297 1106 44-45 0.17623090840522693 1106 46-47 0.15040986861648875 1106 48-49 0.10339116309259921 1106 50-51 0.26536198696514646 1106 52-53 0.16646990770096437 1106 54-55 0.11204056958294473 1106 56-57 0.1352652900292277 1106 58-59 -0.14547673502240954 1106 60-61 -0.07306096258131234 1106 62-63 -0.021783998540648497 1106 64-65 -0.056170850000725636 1106 66-67 -0.18045341682492122 1106 68-69 0.11222443749126754 1106 70-71 0.18039346969628056 1106 72-73 0.10913522332213788 1106 74-75 0.10383501955904606 1106 76-77 -0.17090126727942234 1107 1 -0.298617899417458 1107 2 -0.23598173793528332 1107 3 -0.08438175648055335 1107 4 -0.24417400479466522 1107 5 -0.13449177244449828 1107 6 -0.38812046771510467 1107 7 -0.12449857644047313 1107 8 -0.18374017651549934 1107 9 -0.004362042221792706 1107 10-11 -0.0949173857840151 1107 12-13 0.15176164885583177 1107 14-15 -0.28621841576406837 1107 16-17 -0.18784765948993254 1107 18-19 -0.0968801796441241 1107 20-21 -0.2618883957513347 1107 22-23 0.0243585835061495 1107 24-25 -0.08379034951193631 1107 26-27 -0.1503095698243655 1107 28-29 -0.17459004706454095 1107 30-31 -0.014137013564784695 1107 32-33 -0.061142795172166586 1107 34-35 -0.12913232341725234 1107 36-37 -0.11845330830766443 1107 38-39 0.08985914983131948 1107 40-41 -0.1453094031564035 1107 42-43 0.031525386564506874 1107 44-45 -0.04360242492810329 1107 46-47 0.0925512827579027 1107 48-49 0.0786613651128043 1107 50-51 0.1652660273691886 1107 52-53 0.14667192790298245 1107 54-55 0.03563652917890181 1107 56-57 -0.04505289178895211 1107 58-59 -0.08874441179008485 1107 60-61 -0.18542207369242547 1107 62-63 -0.18512995813660993 1107 64-65 -0.20735266818254416 1107 66-67 -0.19998371985522212 1107 68-69 -0.22184121907438836 1107 70-71 -0.11100804545523602 1107 72-73 -0.0408268978899855 1107 74-75 -0.016543768319738916 1107 76-77 0.05129065191249893 1108 1 0.5009881989729337 1108 2 0.681917642401757 1108 3 0.6414346998533631 1108 4 0.5915491872252829 1108 5 0.6103522412586173 1108 6 0.5983464458091632 1108 7 0.5004998492125665 1108 8 0.6136838769871105 1108 9 0.4854613790230715 1108 10-11 0.5847533867795249 1108 12-13 0.7099865096835529 1108 14-15 0.667173042459055 1108 16-17 0.7595982774354368 1108 18-19 0.5820349063781904 1108 20-21 0.5517451340605035 1108 22-23 0.6381224692463476 1108 24-25 0.6773709832672168 1108 26-27 0.5374533461180917 1108 28-29 0.7066483027679453 1108 30-31 0.8026562305975418 1108 32-33 0.7907591733912724 1108 34-35 0.6793680481286302 1108 36-37 0.8366061705205254 1108 38-39 0.7926311025253412 1108 40-41 0.754187435554897 1108 42-43 0.8243129882673124 1108 44-45 0.810564241738561 1108 46-47 0.5972585801709371 1108 48-49 0.7499007225273466 1108 50-51 0.7371333917864291 1108 52-53 0.8388556101116045 1108 54-55 0.7596440608547645 1108 56-57 0.7782345335869998 1108 58-59 0.6671483688844937 1108 60-61 0.7180570756564251 1108 62-63 0.6951071070287611 1108 64-65 0.7349978948039286 1108 66-67 0.9001683620611978 1108 68-69 0.8988246037423089 1108 70-71 0.8587101779257083 1108 72-73 0.7692100362897172 1108 74-75 0.8377834817369383 1108 76-77 1.023204302130388 1109 1 0.05183045684945853 1109 2 -0.028937818692185147 1109 3 0.08467942211989055 1109 4 0.20904005473668263 1109 5 0.039553541364902856 1109 6 0.020952849369614057 1109 7 -0.01105612469054762 1109 8 0.11327246094204213 1109 9 0.03447950817770362 1109 10-11 0.04636001776381704 1109 12-13 -0.022698876281552316 1109 14-15 0.0737024362007972 1109 16-17 0.15276296704137593 1109 18-19 0.008886160131076792 1109 20-21 0.19693551466377102 1109 22-23 0.11848821100341667 1109 24-25 0.11872662677403412 1109 26-27 -0.03475727678632978 1109 28-29 0.2889143365435487 1109 30-31 0.25705735571381894 1109 32-33 0.23865917481266763 1109 34-35 0.17258797487919963 1109 36-37 0.29511296717676316 1109 38-39 0.3204249807177675 1109 40-41 0.2294067258345791 1109 42-43 0.17384151811432602 1109 44-45 0.20590939690005428 1109 46-47 0.16228679494116705 1109 48-49 0.3015606796642736 1109 50-51 0.14123574672871086 1109 52-53 0.18090837086330325 1109 54-55 0.3174908545734425 1109 56-57 0.29589818004400925 1109 58-59 0.27977173447305503 1109 60-61 0.34114845266040206 1109 62-63 0.026649609227980875 1109 64-65 0.1610719196847299 1109 66-67 0.09535447846745981 1109 68-69 0.03899665619508852 1109 70-71 0.09957966348981984 1109 72-73 -0.017125068001487165 1109 74-75 0.0643078926854983 1109 76-77 0.09444304346577681 1110 1 -0.2596729867142642 1110 2 -0.32003000203321363 1110 3 -0.39804404158323337 1110 4 -0.1987947152100773 1110 5 -0.30221620217223943 1110 6 -0.44138490011861364 1110 7 -0.27051586856703835 1110 8 -0.5027874372018317 1110 9 -0.5586633332780551 1110 10-11 -0.4823262425637296 1110 12-13 -0.4691975290156023 1110 14-15 -0.29853111226550766 1110 16-17 -0.43221392144725 1110 18-19 -0.428173242725272 1110 20-21 -0.5589609424099748 1110 22-23 -0.5911215489441801 1110 24-25 -0.6987959518933664 1110 26-27 -0.5344991477225562 1110 28-29 -0.42495169332850935 1110 30-31 -0.2131887896033149 1110 32-33 -0.2967626526877254 1110 34-35 -0.4603856681225835 1110 36-37 -0.4018522814831158 1110 38-39 -0.5195189858299258 1110 40-41 -0.6156418668663513 1110 42-43 -0.6201570002172225 1110 44-45 -0.6543665228610784 1110 46-47 -0.6151131095091387 1110 48-49 -0.5867531274953954 1110 50-51 -0.7410947659949159 1110 52-53 -0.6257626831077872 1110 54-55 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26-27 -0.21788404879125522 2314 28-29 -0.26973852551998334 2314 30-31 -0.4589011102276075 2314 32-33 -0.6004720049918859 2314 34-35 -0.6226873355640379 2314 36-37 -1.1520607434182573 2314 38-39 -0.8432416365131346 2314 40-41 -0.6300300271175345 2314 42-43 -0.4885967205829225 2314 44-45 -0.600564450244562 2314 46-47 -0.39753949847212056 2314 48-49 -0.49295271876394153 2314 50-51 -0.40783843497577976 2314 52-53 -0.22110393196148337 2314 54-55 -0.3522294726111497 2314 56-57 -0.5378127690424996 2314 58-59 -0.36477209367219743 2314 60-61 -0.24664746047161046 2314 62-63 -0.2620582601440269 2314 64-65 -0.13336916223701323 2314 66-67 -0.07285004129749595 2314 68-69 -0.09402028824712616 2314 70-71 -0.07568247967081021 2314 72-73 -0.06953566275381107 2314 74-75 -0.022441351748970106 2314 76-77 0.05219366942943893 2315 1 -1.1251503600492754 2315 2 -0.9663600427689545 2315 3 -0.9388328830491091 2315 4 -0.9063684787585302 2315 5 -1.0838679733483332 2315 6 -0.8201265004118952 2315 7 -1.340698165315409 2315 8 -1.4572705117488098 2315 9 -1.0309912460561037 2315 10-11 -1.1750398108851883 2315 12-13 -1.1025418135042244 2315 14-15 -1.0384370688195972 2315 16-17 -1.0361054096804594 2315 18-19 -1.0488467099847298 2315 20-21 -1.0128363717922966 2315 22-23 -1.2019903199814266 2315 24-25 -1.3261116094009466 2315 26-27 -1.3878979939165141 2315 28-29 -1.3032595127093032 2315 30-31 -1.3765536882821934 2315 32-33 -1.3112404105394475 2315 34-35 -1.4296182533864439 2315 36-37 -1.3938839564468637 2315 38-39 -1.3883987882100897 2315 40-41 -1.3222676143866678 2315 42-43 -1.235925960057937 2315 44-45 -1.2340691270180812 2315 46-47 -1.109094559297077 2315 48-49 -1.1713906293268117 2315 50-51 -1.0644091784617018 2315 52-53 -0.9752392669359473 2315 54-55 -1.1952134155392642 2315 56-57 -1.3967318761060845 2315 58-59 -1.2972273549303104 2315 60-61 -1.3862242640336682 2315 62-63 -1.1832363541906687 2315 64-65 -1.0834676973262667 2315 66-67 -0.9746411603601679 2315 68-69 -1.0059193811413927 2315 70-71 -1.081875393215519 2315 72-73 -1.2296904939998612 2315 74-75 -1.3512517675468665 2315 76-77 -1.1872866728763007 2316 1 -2.0855757809854296 2316 2 -1.65829547160223 2316 3 -1.9870900318952494 2316 4 -2.947155656170814 2316 5 -2.322657836757351 2316 6 -2.4911345535273597 2316 7 -2.286780802369485 2316 8 -1.889849434230257 2316 9 -2.240936502420112 2316 10-11 -2.033830600499982 2316 12-13 -2.605898090741981 2316 14-15 -4.035691356177381 2316 16-17 -2.987373885048271 2316 18-19 -2.3489797301955164 2316 20-21 -2.1282614149339807 2316 22-23 -2.601032367286173 2316 24-25 -2.592263845944153 2316 26-27 -2.5319760048164923 2316 28-29 -3.1946298950860452 2316 30-31 -3.2597844280768733 2316 32-33 -3.1132608796870436 2316 34-35 -2.9409575713090135 2316 36-37 -3.0084629228012183 2316 38-39 -3.1552843725484365 2316 40-41 -3.0020505531888375 2316 42-43 -3.2958539109982787 2316 44-45 -2.9649518133073087 2316 46-47 -3.0216092445333587 2316 48-49 -2.9128274913416483 2316 50-51 -2.9266479518727735 2316 52-53 -2.9719628140115724 2316 54-55 -3.0122621827106357 2316 56-57 -2.832366972967712 2316 58-59 -2.932141108111093 2316 60-61 -2.7975525527953806 2316 62-63 -2.8487182493051755 2316 64-65 -2.7126609111628035 2316 66-67 -2.654598676763758 2316 68-69 -2.87271057779925 2316 70-71 -2.772462034890875 2316 72-73 -2.6125620243842853 2316 74-75 -2.856436387168781 2316 76-77 -3.1682644171265153 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36878.0 3 4076.0 4 6338.0 5 8110.0 6 8682.0 7 8934.0 8 11028.0 9 12658.0 10 17482.0 11 20328.0 12 14171.0 13 9702.0 14 8860.0 15 8541.0 16 8656.0 17 9012.0 18 9502.0 19 10038.0 20 10570.0 21 11353.0 22 12497.0 23 13928.0 24 15988.0 25 18616.0 26 21668.0 27 25279.0 28 29506.0 29 34553.0 30 40356.0 31 48548.0 32 58371.0 33 72290.0 34 97045.0 35 126983.0 36 172991.0 37 244140.0 38 238253.0 39 68806.0 40 535.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 20.56897738209206 27.097368481194913 32.28346406705891 20.050190069654118 2 20.403978328193027 27.34643528741047 32.33324910769497 19.916337276701537 3 20.61227521719587 27.590351195751822 31.559683469242117 20.23769011781019 4 20.13865784186987 27.423242300102352 32.215058706401955 20.22304115162582 5 20.40889146237014 27.56077786010378 31.987398407277535 20.042932270248546 6 20.478449518299783 27.461864605859134 32.17061596931137 19.889069906529713 7 20.540840155581545 27.182389266244822 32.03066170410808 20.246108874065552 8 20.030832294161417 27.609443215498437 31.90805931787994 20.45166517246021 9 20.37136743725357 27.28658594657744 31.893154877173824 20.44889173899517 10-11 20.567169865231676 27.53032684096068 31.79014873724146 20.112354556566185 12-13 20.791724645010625 27.151706904669158 31.501646251622677 20.55492219869754 14-15 20.41052976077296 27.701362835133864 31.564649517454214 20.32345788663896 16-17 20.606783180667804 27.577498507309183 31.55165884690309 20.264059465119917 18-19 20.506633618672794 27.601370848393387 31.196705189504296 20.69529034342952 20-21 20.601368047072807 27.809296218887532 30.950287348294577 20.639048385745088 22-23 20.790266898407182 27.92193836654683 30.242771997492124 21.045022737553868 24-25 20.795036742821203 28.23928756357014 29.85005398640641 21.11562170720224 26-27 20.955379800882383 28.42051263167051 29.446480712663543 21.177626854783565 28-29 21.019291057141807 28.521890835954274 29.321410114948947 21.13740799195497 30-31 20.860735642079025 28.481132800174713 29.395590616966988 21.26254094077927 32-33 21.007431843028503 28.796296649004454 29.03278746808168 21.163484039885365 34-35 20.979168822626757 28.30918975475068 29.34624094734844 21.365400475274125 36-37 21.027903139432528 28.517318954650456 29.416684998725483 21.038092907191533 38-39 21.036120405584725 28.372232557253607 29.533304550447298 21.05834248671437 40-41 20.897719401499234 28.39697816413122 29.42794887081137 21.27735356355817 42-43 20.935087429131784 28.66074271508557 29.057372976888846 21.346796878893805 44-45 21.005646750919777 28.354296079973256 29.26554777166516 21.374509397441805 46-47 20.76954896204795 28.446498905873362 29.43297478318261 21.35097734889608 48-49 20.891812958318784 28.368972413574173 29.199104790324114 21.54010983778293 50-51 20.590110326216365 28.601508056194934 29.059607905389317 21.74877371219938 52-53 20.50506939481272 28.54450440645075 29.266122828167628 21.6843033705689 54-55 20.504193143341528 28.433369261711515 29.163450838708037 21.898986756238916 56-57 20.3806335363345 28.658655824135636 29.065790409337716 21.89492023019215 58-59 20.5016044383323 28.630462360143387 28.951794469354404 21.91613873216991 60-61 20.401866343185805 28.299061907620647 29.025116011508928 22.27395573768462 62-63 20.394294239282175 28.229539747705278 29.079517634691747 22.296648378320803 64-65 20.52532545930342 28.024119878105342 29.064141999210776 22.386412663380458 66-67 20.284688583566833 28.207040354957524 29.134530705438717 22.373740356036926 68-69 20.38910873988128 28.019709119357078 29.148008260075542 22.443173880686103 70-71 20.162584223416903 27.81138527830567 29.234672100326225 22.791358397951196 72-73 20.395217245612447 27.582816006250805 29.11858264290218 22.903384105234572 74-75 20.24617636603963 27.45599012956534 29.076041292419568 23.221792211975462 76-77 20.09257770981348 27.290030869825145 29.54151416524092 23.07587725512046 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 17009.0 1 10087.5 2 2886.0 3 2667.5 4 2729.0 5 2765.5 6 2850.5 7 3015.0 8 3143.0 9 3155.0 10 3199.0 11 3335.0 12 3589.0 13 3751.0 14 3950.5 15 4367.5 16 4890.5 17 5196.0 18 5604.0 19 6501.5 20 7583.5 21 9280.0 22 10384.0 23 11443.5 24 13759.0 25 16994.5 26 18974.0 27 21344.0 28 25871.5 29 30067.0 30 32105.0 31 35737.0 32 43211.0 33 50243.5 34 55278.5 35 57123.0 36 60508.0 37 65946.0 38 67451.0 39 66903.0 40 67135.5 41 67881.0 42 69650.0 43 70906.0 44 69747.0 45 68040.0 46 67420.5 47 67640.0 48 67931.0 49 66577.0 50 64015.0 51 59288.0 52 55769.0 53 53486.5 54 47921.5 55 41916.5 56 36100.0 57 33006.0 58 31758.5 59 27366.5 60 22518.5 61 20815.0 62 18057.5 63 12482.5 64 8292.5 65 6920.0 66 5890.5 67 4309.5 68 3249.0 69 2282.0 70 1824.0 71 1566.0 72 1146.0 73 926.0 74 868.0 75 825.5 76 575.0 77 296.5 78 226.0 79 181.5 80 140.0 81 113.5 82 71.5 83 59.0 84 58.5 85 49.0 86 35.5 87 31.0 88 27.5 89 21.0 90 18.5 91 19.0 92 16.5 93 11.5 94 7.0 95 9.0 96 13.0 97 12.5 98 11.0 99 29.0 100 48.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0038088660244072134 2 0.008760391856136591 3 0.012442295679730231 4 0.02006002772854466 5 0.014791096394781345 6 0.014346728691933838 7 0.017647745913086755 8 0.019044330122036067 9 0.017901670314713902 10-11 0.02202794184115505 12-13 0.020980503684443066 14-15 0.008665170205526412 16-17 0.006125926189254935 18-19 0.011997927976882723 20-21 0.01142659807322164 22-23 0.014886318045391526 24-25 0.021488352487697364 26-27 0.014156285390713478 28-29 0.008760391856136591 30-31 0.008316024153289083 32-33 0.01003001386427233 34-35 0.011490079173628426 36-37 0.01031567881610287 38-39 0.016632048306578166 40-41 0.01288666338257774 42-43 0.016092458953120475 44-45 0.018758665170205524 46-47 0.01723511876044264 48-49 0.01409280429030669 50-51 0.025201996861494397 52-53 0.030375706544647527 54-55 0.024091077604375625 56-57 0.015235464097628854 58-59 0.016885972708205313 60-61 0.0203456926803752 62-63 0.02586854841576566 64-65 0.019393476174273394 66-67 0.020504395431392167 68-69 0.029709154990376265 70-71 0.05637121716122676 72-73 0.06821044238709251 74-75 0.08116058687007703 76-77 0.07890700780563611 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1575272.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.9040162896359 #Duplication Level Percentage of deduplicated Percentage of total 1 77.98425581609666 44.37617363294297 2 11.901692836098016 13.545082460391287 3 4.25527816607437 7.264272542376837 4 2.004903438990834 4.563482318059258 5 1.1025656570255002 3.1370207053886086 6 0.6351012881981484 2.168388842951771 7 0.42530493215077997 1.6941091151019327 8 0.29169242781950583 1.327877653136368 9 0.19652117456117413 1.006455970663869 >10 1.0619135665779222 11.214767399438157 >50 0.09531101074170997 3.7513491753034796 >100 0.04489986855534185 4.418690474879754 >500 3.3589026601045605E-4 0.12576686400931644 >1k 0.0 0.0 >5k 1.1196342200348536E-4 0.46611467937799345 >10k+ 1.1196342200348536E-4 0.9404481659784802 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14761 0.9370445231045812 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7316 0.4644277305760529 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.5392440162714754E-4 0.0 2 0.0 0.0 0.0 0.0010156976065085902 0.0 3 0.0 0.0 0.0 0.0014600653093560984 0.0 4 6.348110040678689E-5 0.0 0.0 0.0034914605223732792 0.0 5 6.348110040678689E-5 0.0 0.0 0.006348110040678689 0.0 6 6.348110040678689E-5 0.0 0.0 0.01123615477200128 0.0 7 6.348110040678689E-5 0.0 0.0 0.02063135763220574 0.0 8 6.348110040678689E-5 0.0 0.0 0.031677069102986656 0.0 9 6.348110040678689E-5 0.0 0.0 0.04265929947336079 0.0 10 6.348110040678689E-5 0.0 0.0 0.057450395868142136 0.0 11 6.348110040678689E-5 0.0 0.0 0.07249541666455063 0.0 12 6.348110040678689E-5 0.0 0.0 0.09674519701994322 0.0 13 6.348110040678689E-5 0.0 0.0 0.12658131421113306 0.0 14 1.9044330122036069E-4 0.0 0.0 0.16346383354747623 0.0 15 1.9044330122036069E-4 0.0 0.0 0.2049169921131081 0.0 16 1.9044330122036069E-4 0.0 0.0 0.24611622627711277 0.0 17 1.9044330122036069E-4 0.0 0.0 0.28864856354966 0.0 18 1.9044330122036069E-4 0.0 0.0 0.3308634953201733 0.0 19 1.9044330122036069E-4 0.0 0.0 0.373840200295568 0.0 20 1.9044330122036069E-4 0.0 0.0 0.42100665789781067 0.0 21 1.9044330122036069E-4 0.0 0.0 0.4751560365447999 0.0 22 2.5392440162714754E-4 0.0 0.0 0.5340664977222981 0.0 23 2.5392440162714754E-4 0.0 0.0 0.5940561376067117 0.0 24 2.5392440162714754E-4 0.0 0.0 0.6588068600216344 0.0 25 2.5392440162714754E-4 0.0 0.0 0.7245732800430655 0.0 26 2.5392440162714754E-4 0.0 0.0 0.7926885007795479 0.0 27 2.5392440162714754E-4 0.0 0.0 0.8630255600302678 0.0 28 2.5392440162714754E-4 0.0 0.0 0.9328547704777334 0.0 29 2.5392440162714754E-4 0.0 0.0 1.0019222077203176 0.0 30 2.5392440162714754E-4 0.0 0.0 1.0772107928027668 0.0 31 2.5392440162714754E-4 0.0 0.0 1.1603075532352507 0.0 32 2.5392440162714754E-4 0.0 0.0 1.2501333103108543 0.0 33 2.5392440162714754E-4 0.0 0.0 1.3467515451299839 0.0 34 2.5392440162714754E-4 0.0 0.0 1.4507335875963008 0.0 35 2.5392440162714754E-4 0.0 0.0 1.5554774032674992 0.0 36 2.5392440162714754E-4 0.0 0.0 1.6709495249074446 0.0 37 2.5392440162714754E-4 0.0 0.0 1.7863581654469831 0.0 38 3.1740550203393443E-4 0.0 0.0 1.8985292698657756 0.0 39 3.1740550203393443E-4 0.0 0.0 2.0129222127988053 0.0 40 3.1740550203393443E-4 0.0 0.0 2.128140410037124 0.0 41 3.1740550203393443E-4 0.0 0.0 2.2502780472197816 0.0 42 3.8088660244072137E-4 0.0 0.0 2.3865084886927463 0.0 43 3.8088660244072137E-4 0.0 0.0 2.5354351502470682 0.0 44 4.4436770284750826E-4 0.0 0.0 2.691344732846137 0.0 45 4.4436770284750826E-4 0.0 0.0 2.8577286970123255 0.0 46 4.4436770284750826E-4 0.0 0.0 3.0355392592517356 0.0 47 5.078488032542951E-4 0.0 0.0 3.2162699521098577 0.0 48 5.078488032542951E-4 0.0 0.0 3.3899542428228266 0.0 49 5.078488032542951E-4 0.0 0.0 3.576652159119187 0.0 50 5.078488032542951E-4 0.0 0.0 3.751859996241919 0.0 51 5.078488032542951E-4 0.0 0.0 3.94427121157489 0.0 52 5.078488032542951E-4 0.0 0.0 4.145379337663591 0.0 53 5.078488032542951E-4 0.0 0.0 4.361913371151141 0.0 54 5.078488032542951E-4 0.0 0.0 4.591461030222082 0.0 55 5.078488032542951E-4 0.0 0.0 4.830467373253635 0.0 56 5.71329903661082E-4 0.0 0.0 5.088835451909258 0.0 57 5.71329903661082E-4 0.0 0.0 5.34542605975349 0.0 58 7.617732048814427E-4 0.0 0.0 5.608745664240843 0.0 59 7.617732048814427E-4 0.0 0.0 5.877524643363178 0.0 60 7.617732048814427E-4 0.0 0.0 6.145287924879005 0.0 61 8.252543052882296E-4 0.0 0.0 6.424858691070495 0.0 62 8.252543052882296E-4 0.0 0.0 6.7092540208928995 0.0 63 8.252543052882296E-4 0.0 0.0 7.00678993849951 0.0 64 8.252543052882296E-4 0.0 0.0 7.31619682188219 0.0 65 8.252543052882296E-4 0.0 0.0 7.6442036676840575 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 55 2.7534197E-4 32.275673 52 CGCACGA 50 0.00673716 28.392672 4 CGGTATA 180 0.0 27.604862 10 AACGCGA 65 7.324972E-4 27.309319 53 ACGCGAA 75 0.0016884501 23.665821 54 CGGTTAC 165 2.2664608E-9 23.66056 4 GCGGTAT 215 9.458745E-11 21.460258 9 TAATCGA 120 5.121626E-5 20.711538 46 TCGCTAA 125 7.011717E-5 19.883078 57 CGTTTAG 110 6.7706703E-4 19.375248 67 CGCGAAT 110 6.8011374E-4 19.36233 55 TCGATTA 240 4.1472958E-10 19.232143 59 TAGAGCG 285 2.1827873E-10 17.43908 45 TATCGAT 265 1.5570549E-9 17.41779 57 CGATTAT 290 2.8012437E-10 17.140585 60 CCGTCAC 450 0.0 16.57713 68 ATGTTCG 135 0.002682523 15.776712 55 GTAGCCG 590 0.0 15.643508 23 TTTATCG 275 4.6355126E-8 15.489863 55 CCTACGA 185 7.99502E-5 15.354215 61 >>END_MODULE