FastQCFastQC Report
Thu 26 May 2016
SRR936090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575272
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133300.8462030684224693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100980.641032151907734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71830.4559847442219502No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20570.13058062353676064No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20410.12956492593025204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11750.052.17793100-101
TACGCAG8400.046.41405100-101
CGGTATC8900.045.812927100-101
ACCGTAC1500.041.66370898-99
CGAGTAC8400.041.453773100-101
CGAACGC659.1495167E-1041.20586898-99
AGGTATC19050.041.088306100-101
TCGAGTA3700.041.022858100-101
ACGTATC4300.040.835796100-101
GGTATCA91450.040.3464971
TCCGGTA1800.039.67972698-99
CCGTACA4150.039.443195100-101
GCGTATC2650.039.30788100-101
ACGGTAT2500.039.285423100-101
CCCGGTA2600.038.91665398-99
AGTATCA30900.038.237503100-101
CCGGTAT4100.037.746525100-101
CGACGCA801.6916601E-1037.202103100-101
GACGCAG5600.037.202103100-101
CCGTCGA401.19573815E-437.1991596-97
Produced by FastQC (version 0.11.5)