FastQCFastQC Report
Thu 26 May 2016
SRR936089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63528
Sequences flagged as poor quality0
Sequence length77
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18962.9845107669059314No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14752.321810855056038No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAGTA150.002090797571.0275863
AGATTGC150.002090797571.0275838
CGAGAAT150.002090797571.027583
ACGAGAA150.002090797571.027582
CATTGGT150.002097347870.9716625
AACGAGT150.002097347870.9716666
ACGAGTG150.002097347870.9716667
ACTGCAC252.2175048E-456.7773248
AACCCGG200.00653429353.2706878
GAGATTG200.00653429353.27068737
ATTTCAC200.00653429353.27068730
TCAAACG200.00653429353.27068763
CAAACGA200.00655470653.2287464
AAACGAG200.00655470653.2287465
TTCATAT350.001160595340.5871910
AAGGCTT400.002245219735.4858321
TACCCTT552.7318098E-432.25984247
AAGGTGA450.003999046531.54295723
AATATGA450.003999046531.54295750
ATATGAA500.006693918328.3886651