##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936089_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 63528 Sequences flagged as poor quality 0 Sequence length 77 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.8656497922176 39.0 34.0 41.0 10.0 41.0 2 33.6291241657222 38.0 34.0 41.0 9.0 41.0 3 33.4960962095454 38.0 33.0 40.0 9.0 41.0 4 33.31809595768795 38.0 33.0 40.0 8.0 41.0 5 33.16569071905302 38.0 33.0 40.0 6.0 41.0 6 32.918225034630396 38.0 33.0 40.0 2.0 41.0 7 32.57303866011837 37.0 33.0 40.0 2.0 41.0 8 32.1720186374512 37.0 32.0 40.0 2.0 41.0 9 31.925796499181462 36.0 32.0 40.0 2.0 41.0 10-11 31.944740901649666 36.0 32.0 40.0 2.0 41.0 12-13 31.763474373504597 37.0 32.0 40.0 2.0 41.0 14-15 31.452257272383832 36.5 31.5 40.0 2.0 41.0 16-17 30.98628951013726 36.0 30.5 40.0 2.0 41.0 18-19 30.47244522100491 36.0 30.0 40.0 2.0 41.0 20-21 29.874134240020148 35.0 28.0 40.0 2.0 41.0 22-23 29.134649288502708 35.0 24.5 40.0 2.0 41.0 24-25 28.528223775343157 35.0 21.5 40.0 2.0 41.0 26-27 27.523021344918774 34.5 17.0 39.0 2.0 40.5 28-29 27.408701674852033 35.0 13.5 39.0 2.0 40.0 30-31 27.56153192293162 35.0 8.5 40.0 2.0 41.0 32-33 27.289470784535954 35.0 3.5 40.0 2.0 41.0 34-35 26.928275720941947 35.0 2.0 39.0 2.0 41.0 36-37 26.576257713134368 35.0 2.0 39.0 2.0 41.0 38-39 26.215338118624857 35.0 2.0 39.0 2.0 41.0 40-41 25.80124512026193 34.0 2.0 38.0 2.0 40.5 42-43 25.36744427653948 34.0 2.0 38.0 2.0 40.0 44-45 24.95331979599547 34.0 2.0 37.0 2.0 40.0 46-47 24.48616358141292 33.0 2.0 36.5 2.0 40.0 48-49 24.02715338118625 33.0 2.0 36.0 2.0 39.0 50-51 23.5993184107795 33.0 2.0 35.5 2.0 39.0 52-53 23.14541619443395 32.0 2.0 35.0 2.0 38.5 54-55 22.689483377408386 32.0 2.0 35.0 2.0 37.0 56-57 22.256249212945473 31.0 2.0 35.0 2.0 37.0 58-59 21.831633295554717 31.0 2.0 35.0 2.0 36.0 60-61 21.45664903664526 31.0 2.0 35.0 2.0 36.0 62-63 21.113690026445035 30.0 2.0 35.0 2.0 35.5 64-65 20.713708915753685 30.0 2.0 35.0 2.0 35.0 66-67 20.271116672963103 29.0 2.0 34.5 2.0 35.0 68-69 19.88225664274021 29.0 2.0 34.0 2.0 35.0 70-71 19.451635499307393 28.5 2.0 34.0 2.0 35.0 72-73 18.994223019770807 27.0 2.0 34.0 2.0 35.0 74-75 18.43435965243672 25.5 2.0 34.0 2.0 35.0 76-77 17.1770243042438 21.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.018832318550821 1101 2 0.5292845670369033 1101 3 1.216007652023258 1101 4 1.2851104327578753 1101 5 0.10964868884261847 1101 6 0.6303595718844477 1101 7 0.3331226146533979 1101 8 0.6311867787486491 1101 9 -0.0017961089593185875 1101 10-11 0.4693020529572465 1101 12-13 0.631678545769752 1101 14-15 0.18392609956405792 1101 16-17 0.5444608776763395 1101 18-19 0.5567052082974335 1101 20-21 0.37364106496275085 1101 22-23 0.7086416995982283 1101 24-25 -0.4416816686821754 1101 26-27 -0.7192088872760465 1101 28-29 -0.6667175466124178 1101 30-31 -0.7948083066521221 1101 32-33 -0.3951010900764551 1101 34-35 0.2713065570321014 1101 36-37 0.7737831107147812 1101 38-39 0.26435036921096255 1101 40-41 0.3671494093265437 1101 42-43 0.7725555546440539 1101 44-45 0.2432154788858263 1101 46-47 0.2507300433524371 1101 48-49 -0.18779567798058494 1101 50-51 0.16346419367234333 1101 52-53 -0.23539793627330852 1101 54-55 -0.35585020216750607 1101 56-57 -0.5252078027344105 1101 58-59 -0.4361274062407041 1101 60-61 -0.3696349675455046 1101 62-63 -0.9596910984344262 1101 64-65 -0.15926103611755948 1101 66-67 -0.2055675114011315 1101 68-69 -0.27686710315435903 1101 70-71 -0.7411807888841793 1101 72-73 -0.6651645172753788 1101 74-75 -0.9088632701003974 1101 76-77 -1.0449798395578256 1102 1 2.6783764780949824 1102 2 2.297090834843175 1102 3 2.0422184782340835 1102 4 1.3413782390256799 1102 5 1.65167148086541 1102 6 2.1424678339927112 1102 7 2.284689566220351 1102 8 2.734463132025006 1102 9 2.5281184209552094 1102 10-11 2.645940229595425 1102 12-13 3.412661451752655 1102 14-15 2.8876298032677603 1102 16-17 2.91732412553959 1102 18-19 3.537474439066667 1102 20-21 3.150706592028275 1102 22-23 1.6249522409087689 1102 24-25 2.185455083454574 1102 26-27 1.2811472380800808 1102 28-29 2.072242567347697 1102 30-31 1.8512030893592737 1102 32-33 2.207106887131527 1102 34-35 3.124226784952331 1102 36-37 2.7769882389199054 1102 38-39 3.235148090008682 1102 40-41 3.352548269725407 1102 42-43 3.928894585983084 1102 44-45 3.7275459632163113 1102 46-47 3.9875534051758024 1102 48-49 3.824668709483799 1102 50-51 2.862894393102547 1102 52-53 2.8344026335272616 1102 54-55 3.0098905385732344 1102 56-57 2.6920286645020575 1102 58-59 2.711664616551314 1102 60-61 2.9968180239074833 1102 62-63 3.0933715796282506 1102 64-65 3.2175195906630663 1102 66-67 3.900557844724222 1102 68-69 3.7904405891533344 1102 70-71 3.473206675503288 1102 72-73 3.4024993003884383 1102 74-75 2.662005675768551 1102 76-77 2.7434816989037145 1103 1 2.055869355587859 1103 2 0.9737290114813462 1103 3 1.0863780223936317 1103 4 1.63696228460973 1103 5 1.350389429583359 1103 6 1.5562854978103715 1103 7 2.0738633553941384 1103 8 2.3719275194893896 1103 9 2.0537594465962385 1103 10-11 2.2193020529572465 1103 12-13 2.6409378050290044 1103 14-15 2.6561483217862794 1103 16-17 2.627794211009668 1103 18-19 3.1770755786678 1103 20-21 2.9384558797775675 1103 22-23 3.2549379958945224 1103 24-25 2.4842442572437484 1103 26-27 2.3178281497609916 1103 28-29 2.87957874968388 1103 30-31 2.41815465631084 1103 32-33 2.8456396506642854 1103 34-35 2.7064917422172883 1103 36-37 2.931190518122186 1103 38-39 2.8199059247665197 1103 40-41 2.904186446363582 1103 42-43 2.754037036125535 1103 44-45 3.122845108515456 1103 46-47 2.0933226359450323 1103 48-49 1.6640561738712627 1103 50-51 2.0430938233019766 1103 52-53 2.7275650266896534 1103 54-55 2.6534090570917535 1103 56-57 2.4655329380063336 1103 58-59 2.545354075240777 1103 60-61 2.509994662084125 1103 62-63 2.48475334601002 1103 64-65 1.812961186104662 1103 66-67 2.5259139700803495 1103 68-69 2.4638736375863814 1103 70-71 0.39770810000471 1103 72-73 0.7422428901320295 1103 74-75 0.2578033965662705 1103 76-77 0.2790942345162506 1104 1 0.772644954716398 1104 2 1.634949055054335 1104 3 0.4894281313260933 1104 4 1.2633239403831453 1104 5 1.556271782524533 1104 6 1.227309462951986 1104 7 2.366891677834225 1104 8 2.681295711210524 1104 9 0.8576810152236902 1104 10-11 0.9012192642862189 1104 12-13 0.7869073039396852 1104 14-15 1.5984141169932506 1104 16-17 1.590212511663264 1104 18-19 2.1449405024150856 1104 20-21 1.767976576945319 1104 22-23 1.757116645132001 1104 24-25 2.336096109095603 1104 26-27 2.2176102848372423 1104 28-29 1.688946941405014 1104 30-31 2.4383071617574643 1104 32-33 1.6190601299671208 1104 34-35 1.9875374938512742 1104 36-37 2.518881149930465 1104 38-39 2.891256687293751 1104 40-41 2.8448182546424476 1104 42-43 2.6587211319860984 1104 44-45 2.817289552959899 1104 46-47 2.544303028101897 1104 48-49 3.37048318912181 1104 50-51 3.5665143026048085 1104 52-53 3.99880685675501 1104 54-55 4.022145440534018 1104 56-57 3.4344871863723476 1104 58-59 3.4663780403823843 1104 60-61 3.651606862519852 1104 62-63 3.38126750723006 1104 64-65 3.5689524715077106 1104 66-67 3.7165457783592153 1104 68-69 3.321172112531915 1104 70-71 2.8514118037084124 1104 72-73 2.301066419543794 1104 74-75 0.19952452946387567 1104 76-77 -0.8096857219107676 1105 1 0.3268245212798675 1105 2 0.4347426567055237 1105 3 0.5503156442259893 1105 4 0.19934045225105024 1105 5 0.850389429583359 1105 6 0.5348430026836652 1105 7 0.23273275110563674 1105 8 -0.5715422660856575 1105 9 -0.43746862357920335 1105 10-11 -0.20662387296868445 1105 12-13 -0.42826297469808594 1105 14-15 -0.1781596704164521 1105 16-17 0.6000164332318967 1105 18-19 0.3023192433851527 1105 20-21 -0.03181702470586245 1105 22-23 -0.027713076230231337 1105 24-25 -0.4884652944131709 1105 26-27 -1.0486435851317957 1105 28-29 -0.40599629904906465 1105 30-31 -0.4068940766326321 1105 32-33 -1.074438322045264 1105 34-35 -0.6940930531043499 1105 36-37 -0.5770940822676778 1105 38-39 -0.35796931889819916 1105 40-41 -0.4812911364824224 1105 42-43 -1.485241716311112 1105 44-45 -2.4283244821278167 1105 46-47 -1.7663264868619883 1105 48-49 -1.3101153660897502 1105 50-51 -1.8901420441444188 1105 52-53 -1.778282926526721 1105 54-55 -1.6852849000232553 1105 56-57 -1.34002261754922 1105 58-59 -1.2524042093596144 1105 60-61 -1.4939039733934614 1105 62-63 -1.940197921046515 1105 64-65 -1.4774871569752612 1105 66-67 -0.8113179987305692 1105 68-69 -0.8733583312245337 1105 70-71 -0.613500476993341 1105 72-73 -0.7402132502188508 1105 74-75 -0.9005786697105336 1105 76-77 -0.044979839557825585 1106 1 -0.06035031235594346 1106 2 -0.0026439131674393934 1106 3 0.6138365153693641 1106 4 -0.4709559784809514 1106 5 -0.5174266623706671 1106 6 -0.7879035187924401 1106 7 0.8854853221885222 1106 8 1.273587800460021 1106 9 1.0652536994697996 1106 10-11 1.2818818741577545 1106 12-13 1.5933643695245436 1106 14-15 1.4738366998194827 1106 16-17 1.6172578125422454 1106 18-19 2.091187966918124 1106 20-21 1.0751097750521517 1106 22-23 2.039740039317259 1106 24-25 1.6406938102450255 1106 26-27 2.452566336223313 1106 28-29 2.715147076631517 1106 30-31 3.1071712591205483 1106 32-33 2.473353571481656 1106 34-35 2.526415113864797 1106 36-37 3.1214842601400647 1106 38-39 3.2218854905391865 1106 40-41 3.5485031768872055 1106 42-43 3.5870510846568244 1106 44-45 2.7971746104311634 1106 46-47 3.0157364290484807 1106 48-49 2.789854385876371 1106 50-51 2.754141077452676 1106 52-53 2.949787248911875 1106 54-55 2.6090284696077184 1106 56-57 3.2059543939450315 1106 58-59 2.9971420701322167 1106 60-61 2.981897599504304 1106 62-63 3.0744723753842216 1106 64-65 3.7858219779309685 1106 66-67 3.7765525396844346 1106 68-69 3.7576156554663314 1106 70-71 2.79681410255899 1106 72-73 2.480748637258465 1106 74-75 2.765785516617356 1106 76-77 1.5670891259594164 1107 1 0.9233157493500457 1107 2 1.2066724812669207 1107 3 0.9889121354540578 1107 4 2.0940772943563175 1107 5 1.8679332892324823 1107 6 1.2190535289994529 1107 7 1.2327327511056367 1107 8 2.3056507163704865 1107 9 2.5625313764208 1107 10-11 1.9688147235225415 1107 12-13 1.273491411266825 1107 14-15 0.8832438383554795 1107 16-17 1.1263322227055816 1107 18-19 1.5654771381219952 1107 20-21 2.0646742033643086 1107 22-23 2.5336904325416967 1107 24-25 2.0466224248850757 1107 26-27 2.600479221885749 1107 28-29 3.453652823757956 1107 30-31 3.8562638181042104 1107 32-33 3.9694213270775442 1107 34-35 3.244503438123722 1107 36-37 3.949221707206007 1107 38-39 4.194662260049167 1107 40-41 4.352042196850913 1107 42-43 3.786688108250292 1107 44-45 4.001500079275694 1107 46-47 4.154726144716964 1107 48-49 4.0846214760155135 1107 50-51 3.1274018155047045 1107 52-53 2.642769705052224 1107 54-55 2.0866449245381453 1107 56-57 2.3178721192928826 1107 58-59 2.572157194149156 1107 60-61 2.2429381318696997 1107 62-63 2.6036617280762933 1107 64-65 2.8909338956563175 1107 66-67 1.9956995451290815 1107 68-69 1.5389223705298534 1107 70-71 2.5882539089715735 1107 72-73 1.7510148199565911 1107 74-75 1.3888950144999939 1107 76-77 2.3848447218456847 1108 1 -1.1293158295973242 1108 2 -0.6413707036183638 1108 3 -0.950089214073607 1108 4 -0.7547756071281668 1108 5 -0.19063621144228193 1108 6 -0.12291678139190765 1108 7 -1.3383873568565718 1108 8 -1.2309214833596123 1108 9 -0.12572773289094386 1108 10-11 0.16517099882619135 1108 12-13 -0.08156931747810603 1108 14-15 -0.37006250442454913 1108 16-17 -0.7845989513834866 1108 18-19 -0.8356024840102592 1108 20-21 -2.1454472541255676 1108 22-23 -1.5615862206296889 1108 24-25 -0.5664679934685033 1108 26-27 -0.2861604542276126 1108 28-29 0.08185795196308021 1108 30-31 0.301203089359273 1108 32-33 0.005183810208446715 1108 34-35 -0.22000398427843848 1108 36-37 0.32506516199683233 1108 38-39 0.32745578231637396 1108 40-41 0.477548269725407 1108 42-43 0.8692792013677 1108 44-45 0.9506228862932353 1108 46-47 -0.11436967174727641 1108 48-49 -0.31186975205466183 1108 50-51 0.339817470025622 1108 52-53 -0.3117512126265858 1108 54-55 -0.24587869219599767 1108 56-57 0.2958748183482136 1108 58-59 -0.2556430757563781 1108 60-61 -0.08972043763097659 1108 62-63 0.20298696424363882 1108 64-65 0.7944426675861429 1108 66-67 -0.23021138604500635 1108 68-69 -1.2153286416158977 1108 70-71 -0.20756255526594458 1108 72-73 0.21403776192690316 1108 74-75 -1.2437635550006796 1108 76-77 -1.4449798395578242 1109 1 0.33842610588654765 1109 2 0.7276119266545891 1109 3 -0.12031253913563944 1109 4 0.45117972785943294 1109 5 0.6299593220564716 1109 6 0.2832866925535029 1109 7 -0.4076181260873426 1109 8 -0.8927080838558901 1109 9 -0.602154531898389 1109 10-11 0.43316107326787545 1109 12-13 -0.11742539688258091 1109 14-15 -0.0881766483451436 1109 16-17 0.6564680461351209 1109 18-19 0.43573746636194954 1109 20-21 -0.7494174774506277 1109 22-23 -0.5751097938306842 1109 24-25 -2.6423985145603126 1109 26-27 -2.6155649207288576 1109 28-29 -2.540970832156024 1109 30-31 -2.3330400868193877 1109 32-33 -2.375089142645148 1109 34-35 -1.9288129172809185 1109 36-37 -1.8579373193927466 1109 38-39 -1.766712952174938 1109 40-41 -2.5112854771728585 1109 42-43 -2.2841947440417307 1109 44-45 -2.018731952416445 1109 46-47 -2.9953870414743236 1109 48-49 -2.286063300441757 1109 50-51 -1.900753249577356 1109 52-53 -2.1066643639913494 1109 54-55 -2.1393030098138617 1109 56-57 -3.1249688541083636 1109 58-59 -3.7787199988332993 1109 60-61 -4.099025648549091 1109 62-63 -3.444159437741476 1109 64-65 -3.189428300155793 1109 66-67 -3.553536448079747 1109 68-69 -4.623641296702747 1109 70-71 -4.815254862958252 1109 72-73 -4.626036679760446 1109 74-75 -3.888552636886537 1109 76-77 -3.5772379040739537 1110 1 -1.5227584525481461 1110 2 -0.9336783959260586 1110 3 -2.765473829458223 1110 4 -2.1204754810247586 1110 5 -2.6960586578483365 1110 6 -3.266422456045124 1110 7 -3.366634519529967 1110 8 -2.9474410293873525 1110 9 -2.6402296244420143 1110 10-11 -3.217552834717317 1110 12-13 -2.9853524196218686 1110 14-15 -3.2148292131256824 1110 16-17 -2.7442458618500716 1110 18-19 -3.3039505294074836 1110 20-21 -4.2153085403802955 1110 22-23 -4.0334651613610895 1110 24-25 -4.259532306204946 1110 26-27 -4.306336998994322 1110 28-29 -5.281319853837672 1110 30-31 -4.632088209505795 1110 32-33 -3.6303773499428758 1110 34-35 -3.6341275656151346 1110 36-37 -5.248074812782491 1110 38-39 -5.16901332234945 1110 40-41 -4.843384895470056 1110 42-43 -4.554554342137976 1110 44-45 -4.087082031739552 1110 46-47 -3.5214314624156238 1110 48-49 -3.3315418832022026 1110 50-51 -4.128278368562022 1110 52-53 -3.017425865842224 1110 54-55 -3.131502904049718 1110 56-57 -3.279913327931734 1110 58-59 -3.2787199988332993 1110 60-61 -4.126920942044595 1110 62-63 -4.359812531342744 1110 64-65 -4.10719667667615 1110 66-67 -4.501712016562031 1110 68-69 -5.121129398236327 1110 70-71 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0.4763796500923938 2314 70-71 0.7691607214573324 2314 72-73 0.6145372624264027 2314 74-75 0.6316610204238948 2314 76-77 0.9809941864162006 2315 1 1.2083465080650129 2315 2 1.7806073183596567 2315 3 1.234526170541777 2315 4 0.5818877295351754 2315 5 1.361211940405866 2315 6 1.6986616401865149 2315 7 0.9235507050814888 2315 8 1.4330145805764545 2315 9 1.5126339054706932 2315 10-11 1.1461899798451753 2315 12-13 1.2482009122921127 2315 14-15 0.36490253054048694 2315 16-17 0.18443201764748096 2315 18-19 0.8359259875182126 2315 20-21 -0.23915354783185805 2315 22-23 -0.2647830238264959 2315 24-25 -0.1660683930689011 2315 26-27 0.3851682171010573 2315 28-29 0.31512468522981507 2315 30-31 -0.5586370704808878 2315 32-33 -0.4258851208604817 2315 34-35 -0.6059180701925229 2315 36-37 -0.49461515768349074 2315 38-39 -1.3562605013999054 2315 40-41 -1.1817923896152571 2315 42-43 -1.2532982212097217 2315 44-45 -1.5506758150054658 2315 46-47 -1.361222818600421 2315 48-49 -1.7456359858208934 2315 50-51 -1.502240472032323 2315 52-53 -1.5761867770621514 2315 54-55 -1.1930315393488407 2315 56-57 -1.2058234833500876 2315 58-59 -0.9215771416904452 2315 60-61 -2.0662439141544517 2315 62-63 -2.7386713774147076 2315 64-65 -2.523739150595677 2315 66-67 -3.409631965465586 2315 68-69 -3.2054385317257896 2315 70-71 -3.126943174646563 2315 72-73 -3.340008192119054 2315 74-75 -3.296910408147534 2315 76-77 -3.642382436960423 2316 1 -5.652392752674249 2316 2 -5.041370703618366 2316 3 -5.088550752535145 2316 4 -5.37016022251278 2316 5 -6.159866980673055 2316 6 -5.138301396776523 2316 7 -6.599925818395036 2316 8 -6.7078445602826875 2316 9 -5.648804655967865 2316 10-11 -6.588675155019963 2316 12-13 -6.381569317478114 2316 14-15 -5.985447119809162 2316 16-17 -6.776906643691181 2316 18-19 -6.820217868625644 2316 20-21 -5.906985715664028 2316 22-23 -6.023124682168152 2316 24-25 -5.604929531930043 2316 26-27 -5.363083531150693 2316 28-29 -5.941218971113841 2316 30-31 -5.875719987563805 2316 32-33 -5.025585420560784 2316 34-35 -5.327696291970746 2316 36-37 -5.736473299541629 2316 38-39 -5.826390371529779 2316 40-41 -5.714759422582286 2316 42-43 -6.43841310632461 2316 44-45 -6.780146344475995 2316 46-47 -6.129754287131892 2316 48-49 -6.042638982823892 2316 50-51 -6.360182529974377 2316 52-53 -6.7502127510881245 2316 54-55 -5.2612633075806094 2316 56-57 -5.004125181651787 2316 58-59 -5.278719998833299 2316 60-61 -4.5512588991694365 2316 62-63 -4.9123976511409815 2316 64-65 -4.913249640106166 2316 66-67 -4.468672924506546 2316 68-69 -5.099944026231281 2316 70-71 -4.6691010168044045 2316 72-73 -6.409039161150023 2316 74-75 -5.820686631923758 2316 76-77 -5.421902916480903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6096.0 3 418.0 4 600.0 5 677.0 6 754.0 7 806.0 8 985.0 9 1066.0 10 1347.0 11 1346.0 12 985.0 13 695.0 14 637.0 15 677.0 16 687.0 17 724.0 18 643.0 19 702.0 20 628.0 21 697.0 22 721.0 23 689.0 24 742.0 25 872.0 26 923.0 27 1016.0 28 1183.0 29 1344.0 30 1524.0 31 1760.0 32 2058.0 33 2575.0 34 3657.0 35 4358.0 36 5197.0 37 6302.0 38 5608.0 39 1815.0 40 14.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.579389817700953 29.627530619312992 33.92840275809956 17.864676804886496 2 17.801640660672955 29.457889184550705 34.69792634346806 18.042543811308278 3 17.955661922156445 29.624322962589744 33.577906537347275 18.84210857790654 4 17.535545023696685 30.208940183590244 34.74358772496103 17.51192706775204 5 17.917873787630683 30.19586849729185 33.779443254817984 18.10681446025948 6 18.315646943980855 30.57436155808168 33.84608117895267 17.26391031898479 7 18.48399439449527 30.463398888346532 32.85833503912831 18.194271678029885 8 17.100974634315318 30.950544000251924 34.15786241753397 17.79061894789879 9 17.756258856872932 30.730593607305934 33.960006298220755 17.55314123760038 10-11 17.637425795581592 30.898956020596152 33.56480387988726 17.898814303934998 12-13 17.966461974492205 30.662887734215083 33.160919540229884 18.209730751062825 14-15 18.05744692574721 30.228512504034192 33.59519517628445 18.118845393934148 16-17 18.126431624436208 30.44371502113491 33.344353397721996 18.08549995670689 18-19 18.482393784096544 30.533185335632023 32.518558754300194 18.465862125971235 20-21 18.41771703445941 30.189971579055104 33.039151622985536 18.35315976349995 22-23 18.142098012202325 30.418421570556976 32.34875024601456 19.090730171226138 24-25 18.549948830984807 30.03306305597103 31.935763205542 19.481224907502163 26-27 18.619316034765085 29.49993701977579 31.88688751731956 19.993859428139565 28-29 18.703999622157326 29.773215677322355 31.56245818147469 19.960326519045633 30-31 18.210585975628955 29.70968859221008 31.538304102774017 20.541421329386942 32-33 18.208440592305696 29.709751316628484 31.431405427107194 20.650402663958623 34-35 18.002046760607733 29.35054711485476 31.520900574667397 21.12650554987011 36-37 17.761526532475813 29.033400769878693 32.07827887241898 21.126793825226517 38-39 17.709670054557908 28.69289330110769 32.55131041323875 21.046126231095645 40-41 17.85854864358478 28.660274274557963 32.2973249570954 21.18385212476186 42-43 17.821774384003778 28.43895142879635 31.970400692749745 21.76887349445013 44-45 18.069889552779287 28.325028143179903 31.788016909524597 21.817065394516213 46-47 17.974759284190306 28.485950699512664 31.598132533440403 21.941157482856624 48-49 18.10409302398816 28.314215759598802 31.44125774478236 22.140433471630676 50-51 18.49518920365967 27.942774357116985 31.08120876179078 22.480827677432565 52-53 18.44179363017204 27.674501003897483 31.386165898980355 22.49753946695012 54-55 18.735090108886492 27.553872438254352 31.03461850361774 22.676418949241416 56-57 18.871192498760028 27.28883080484022 30.843417126571616 22.996559569828136 58-59 18.755018815638238 27.144116767174193 30.83324148572688 23.26762293146069 60-61 18.398822223446885 27.300640061723048 30.64817074610885 23.652366968721214 62-63 19.137712481596083 27.2692916361575 29.852532457818615 23.740463424427805 64-65 18.79620517261741 26.827540054324295 30.19485887493603 24.18139589812227 66-67 18.915833398944965 26.35855444453193 30.394457129359893 24.331155027163216 68-69 19.149907872564924 26.15001811052143 30.32016252224444 24.37991149466921 70-71 18.9902823933347 25.520923566377395 30.808908068605987 24.679885971681916 72-73 19.43503536936965 24.998030658705275 30.527153277771653 25.039780694153418 74-75 19.32081357427568 25.200478943802878 30.384572968033652 25.094134513887795 76-77 19.377964045439505 24.810537427720618 30.781168759551907 25.030329767287967 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2264.0 1 1303.0 2 293.0 3 237.5 4 231.0 5 245.0 6 252.0 7 250.5 8 241.5 9 227.0 10 240.5 11 255.0 12 257.0 13 258.0 14 271.0 15 298.5 16 326.0 17 339.0 18 369.5 19 418.0 20 483.0 21 583.5 22 637.0 23 690.0 24 825.0 25 943.0 26 979.0 27 1071.0 28 1252.5 29 1453.5 30 1565.0 31 1633.5 32 1813.0 33 1953.0 34 2052.5 35 2123.0 36 2166.0 37 2120.0 38 2032.5 39 2034.0 40 2099.5 41 2236.0 42 2354.5 43 2402.0 44 2381.0 45 2406.5 46 2426.5 47 2371.5 48 2343.0 49 2393.0 50 2433.0 51 2193.0 52 1963.0 53 1898.5 54 1772.0 55 1543.0 56 1361.0 57 1346.0 58 1220.0 59 991.5 60 843.0 61 797.0 62 719.0 63 586.5 64 466.0 65 400.0 66 339.0 67 224.5 68 164.0 69 136.0 70 115.0 71 112.5 72 94.5 73 70.5 74 62.0 75 54.0 76 43.0 77 30.0 78 20.0 79 21.0 80 18.5 81 15.0 82 11.0 83 7.0 84 5.5 85 5.0 86 6.0 87 6.0 88 4.5 89 1.5 90 1.5 91 3.0 92 3.0 93 2.5 94 1.0 95 0.0 96 0.0 97 0.0 98 2.5 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009444654325651681 2 0.026759853922679763 3 0.02518574486840448 4 0.026759853922679763 5 0.02518574486840448 6 0.02203752675985392 7 0.029908072031230325 8 0.026759853922679763 9 0.028333962976955045 10-11 0.03305629013978088 12-13 0.028333962976955045 14-15 0.013379926961339881 16-17 0.011805817907064602 18-19 0.021250472232716283 20-21 0.029121017504092683 22-23 0.024398690341266842 24-25 0.020463417705578642 26-27 0.02518574486840448 28-29 0.014954036015615162 30-31 0.0157410905427528 32-33 0.021250472232716283 34-35 0.020463417705578642 36-37 0.018102254124165724 38-39 0.027546908449817407 40-41 0.023611635814129204 42-43 0.020463417705578642 44-45 0.021250472232716283 46-47 0.030695126558367963 48-49 0.027546908449817407 50-51 0.03935272635688201 52-53 0.04013978088401964 54-55 0.033843344666918525 56-57 0.029121017504092683 58-59 0.026759853922679763 60-61 0.029121017504092683 62-63 0.0354174537211938 64-65 0.03226923561264325 66-67 0.03620450824833145 68-69 0.045649162573983125 70-71 0.055093816899634815 72-73 0.08657599798514042 74-75 0.08657599798514042 76-77 0.09287243420224153 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 63528.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.8164588842715 #Duplication Level Percentage of deduplicated Percentage of total 1 86.16328095055576 60.156151618184104 2 7.068293012873989 9.869663770306007 3 2.741640926205668 5.742349829996222 4 1.4610060198859152 4.080090668681526 5 0.7688318715757672 2.6838559375393527 6 0.5388587017789102 2.2572723838307516 7 0.2998669763037449 1.4654955295302858 8 0.21644533627939488 1.2089157536834152 9 0.16909791896827722 1.062523611635814 >10 0.5614050909746804 5.89976073542375 >50 0.00676391675873109 0.26759853922679766 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0045092778391540595 5.3063216219619695 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1896 2.9845107669059314 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1475 2.321810855056038 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.004722327162825841 0.0 2 0.0 0.0 0.0 0.018889308651303362 0.0 3 0.0 0.0 0.0 0.026759853922679763 0.0 4 0.0 0.0 0.0 0.042500944465432566 0.0 5 0.0 0.0 0.0 0.08185367082231457 0.0 6 0.0 0.0 0.0 0.12435461528774713 0.0 7 0.0 0.0 0.0 0.17000377786173027 0.0 8 0.0 0.0 0.0 0.23611635814129203 0.0 9 0.0 0.0 0.0 0.32898879234353356 0.0 10 0.0 0.0 0.0 0.45806573479410656 0.0 11 0.0 0.0 0.0 0.6060319858959828 0.0 12 0.0 0.0 0.0 0.7618687822692356 0.0 13 0.0 0.0 0.0 0.9413172144566175 0.0 14 0.0 0.0 0.0 1.1097468832640724 0.0 15 0.0 0.0 0.0 1.2466943709860219 0.0 16 0.0 0.0 0.0 1.4119758216849263 0.0 17 0.0 0.0 0.0 1.5662385090039037 0.0 18 0.0 0.0 0.0 1.7000377786173027 0.0 19 0.0 0.0 0.0 1.8495781387734542 0.0 20 0.0 0.0 0.0 1.9644880997355496 0.0 21 0.0 0.0 0.0 2.1156025689459765 0.0 22 0.0 0.0 0.0 2.2761616924820554 0.0 23 0.0 0.0 0.0 2.4146832892582797 0.0 24 0.0 0.0 0.0 2.543760231708853 0.0 25 0.0 0.0 0.0 2.6838559375393527 0.0 26 0.0 0.0 0.0 2.8050623347185493 0.0 27 0.0 0.0 0.0 2.923120513789195 0.0 28 0.0 0.0 0.0 3.0537715652940434 0.0 29 0.0 0.0 0.0 3.1718297443646897 0.0 30 0.0 0.0 0.0 3.3103513411409144 0.0 31 0.0 0.0 0.0 3.4803551190026445 0.0 32 0.0 0.0 0.0 3.684989296058431 0.0 33 0.0 0.0 0.0 3.8408260924316835 0.0 34 0.0 0.0 0.0 4.013978088401965 0.0 35 0.0 0.0 0.0 4.177685430046593 0.0 36 0.0 0.0 0.0 4.366578516559628 0.0 37 0.0 0.0 0.0 4.542878730638459 0.0 38 0.0 0.0 0.0 4.714456617554464 0.0 39 0.0 0.0 0.0 4.904923813121773 0.0 40 0.0 0.0 0.0 5.133169625991688 0.0 41 0.0 0.0 0.0 5.314192167233346 0.0 42 0.0 0.0 0.0 5.564475506863116 0.0 43 0.0 0.0 0.0 5.83994459136129 0.0 44 0.0 0.0 0.0 6.115413675859464 0.0 45 0.0 0.0 0.0 6.378289887923436 0.0 46 0.0 0.0 0.0 6.611258027956177 0.0 47 0.0 0.0 0.0 6.882004785291525 0.0 48 0.0 0.0 0.0 7.195252487092306 0.0 49 0.0 0.0 0.0 7.459702808210553 0.0 50 0.0 0.0 0.0 7.691096839189019 0.0 51 0.0 0.0 0.0 8.0106409772069 0.0 52 0.0 0.0 0.0 8.303425261302102 0.0 53 0.0 0.0 0.0 8.626117617428536 0.0 54 0.0 0.0 0.0 8.973995718423373 0.0 55 0.0 0.0 0.0 9.299836292658355 0.0 56 0.0 0.0 0.0 9.715401082987029 0.0 57 0.0 0.0 0.0 10.1120765646644 0.0 58 0.0 0.0 0.0 10.496159173907568 0.0 59 0.0 0.0 0.0 10.883390001259288 0.0 60 0.0 0.0 0.0 11.267472610502455 0.0 61 0.0 0.0 0.0 11.678315073668303 0.0 62 0.0 0.0 0.0 12.081286991562775 0.0 63 0.0 0.0 0.0 12.477962473240146 0.0 64 0.0 0.0 0.0 12.934454098979977 0.0 65 0.0 0.0 0.0 13.386223397556982 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCAGTA 15 0.0020907975 71.02758 63 AGATTGC 15 0.0020907975 71.02758 38 CGAGAAT 15 0.0020907975 71.02758 3 ACGAGAA 15 0.0020907975 71.02758 2 CATTGGT 15 0.0020973478 70.97166 25 AACGAGT 15 0.0020973478 70.97166 66 ACGAGTG 15 0.0020973478 70.97166 67 ACTGCAC 25 2.2175048E-4 56.77732 48 AACCCGG 20 0.006534293 53.270687 8 GAGATTG 20 0.006534293 53.270687 37 ATTTCAC 20 0.006534293 53.270687 30 TCAAACG 20 0.006534293 53.270687 63 CAAACGA 20 0.006554706 53.22874 64 AAACGAG 20 0.006554706 53.22874 65 TTCATAT 35 0.0011605953 40.58719 10 AAGGCTT 40 0.0022452197 35.48583 21 TACCCTT 55 2.7318098E-4 32.259842 47 AAGGTGA 45 0.0039990465 31.542957 23 AATATGA 45 0.0039990465 31.542957 50 ATATGAA 50 0.0066939183 28.38866 51 >>END_MODULE