FastQCFastQC Report
Thu 26 May 2016
SRR936089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63528
Sequences flagged as poor quality0
Sequence length125
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT11151.7551315955169375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9431.4843848381815894No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8381.3191033874826847No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8151.2828988792343534No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT7281.145951391512404TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG4760.7492759098350333TruSeq Adapter, Index 3 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.7256642740209042No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.6327918398186626No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT4010.6312177307643874RNA PCR Primer, Index 3 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390.37621206397179197No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.3415816647777358No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT1980.31167359274650547TruSeq Adapter, Index 3 (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.2849137388238257No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1510.2376904671955673No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA1190.18731897745875833RNA PCR Primer, Index 3 (96% over 28bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1100.17315199597028083No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG1090.17157788691600553TruSeq Adapter, Index 3 (95% over 24bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.14639214204760106No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA850.13379926961339883No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT800.1259287243420224No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA780.12278050623347185No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.12278050623347185No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.12120639717919657No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA760.1196322881249213No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.11018763379926963No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA690.10861352474499433No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT650.1023170885278932No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGC152.6816814E-4119.090624
CTTAGCG152.6816814E-4119.090623
TAGCGCC152.6901116E-4118.996845
CGCCATC208.4453466E-489.247648
CCTGTCG201.7661869E-459.4984396-97
CCCGTAC257.415194E-659.49842598-99
TACGCAG501.8189894E-1259.498425100-101
TCCGGTA257.415194E-659.49842598-99
GAAGCGC150.00422833659.4984272-73
AAGCGCA150.00422833659.4984274-75
CTACGCA150.00422833659.49842100-101
AGCGCAA150.00422833659.4984274-75
TTGCGGT150.00422833659.4984266-67
CACGTAC150.00422833659.4984298-99
ATACGCA150.00422833659.49842100-101
GATTAGT150.00422833659.4984282-83
TCGGTAT150.00422833659.49842100-101
GCGTACA150.00422833659.49842100-101
CTTACGC150.00422833659.4984298-99
GCGAGTA150.00422833659.49842100-101
Produced by FastQC (version 0.11.5)