FastQCFastQC Report
Thu 26 May 2016
SRR936088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62812
Sequences flagged as poor quality0
Sequence length77
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18822.996242756161243No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15442.458129019932497No Hit
TTAAAAACAAAAATGAAGTATTAAGGCGGAAGATTTAAAAAAATTTTTGC680.10825956823536904No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC150.002094479570.99522434
CTCGTTA150.002094479570.99522433
GCTGCCT150.002094479570.99522428
TACGGAT150.002094479570.99522438
CGTTACG150.002094479570.99522435
CGGATGG150.002094479570.99522440
ACGGATG150.002094479570.99522439
TTACGGA150.002094479570.99522437
CTTGTTG200.00652515853.2888468
GTAGTCG200.00652515853.2888468
TACTTTA200.006545770453.24641822
AGTCAGT200.006545770453.2464185
CAAGTCA200.006545770453.2464183
GGCGTCA200.006545770453.24641823
GTTACGG200.006545770453.24641836
CGTCAGA200.006545770453.24641825
TGCCTCA500.00668251828.39808830
GTCGCCG950.006548195618.69784271
AGCAGTG2050.002956633212.1211351
GGTATCA2250.00592593111.0437017
Produced by FastQC (version 0.11.5)