##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936088_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 62812 Sequences flagged as poor quality 0 Sequence length 77 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.960135006049796 39.0 34.0 41.0 11.0 41.0 2 33.750445774692736 38.0 34.0 41.0 10.0 41.0 3 33.52910271922563 38.0 33.0 40.0 10.0 41.0 4 33.3670795389416 38.0 33.0 40.0 9.0 41.0 5 33.243010889638924 38.0 33.0 40.0 8.0 41.0 6 33.007625931350695 38.0 33.0 40.0 7.0 41.0 7 32.7203559829332 37.0 33.0 40.0 2.0 41.0 8 32.38086671336687 37.0 33.0 40.0 2.0 41.0 9 32.299687957715086 37.0 33.0 40.0 2.0 41.0 10-11 32.23263866777049 37.0 33.0 40.0 2.0 41.0 12-13 31.885778195249316 37.0 32.0 40.0 2.0 41.0 14-15 31.523060880086607 36.5 31.5 40.0 2.0 41.0 16-17 31.02824301088964 36.0 30.5 40.0 2.0 41.0 18-19 30.537174425269058 36.0 30.0 40.0 2.0 41.0 20-21 29.900488760109532 35.5 28.0 40.0 2.0 41.0 22-23 29.159022161370437 35.0 24.5 40.0 2.0 41.0 24-25 28.576697127937337 35.0 22.0 40.0 2.0 41.0 26-27 27.56789307775584 34.5 17.5 39.0 2.0 40.5 28-29 27.4521349423677 35.0 14.5 39.0 2.0 40.0 30-31 27.622341272368338 35.0 10.0 40.0 2.0 41.0 32-33 27.356548111825767 35.0 4.5 40.0 2.0 41.0 34-35 27.017114564096033 35.0 2.0 39.0 2.0 41.0 36-37 26.66578838438515 35.0 2.0 39.0 2.0 41.0 38-39 26.282016175253133 35.0 2.0 39.0 2.0 41.0 40-41 25.859859580971786 34.0 2.0 38.0 2.0 40.5 42-43 25.40754951283194 34.0 2.0 38.0 2.0 40.0 44-45 24.99426861109342 34.0 2.0 37.0 2.0 40.0 46-47 24.528688785582375 33.0 2.0 36.5 2.0 39.5 48-49 24.134950327962812 33.0 2.0 36.0 2.0 39.0 50-51 23.675921798382475 33.0 2.0 35.5 2.0 39.0 52-53 23.197621473603768 32.0 2.0 35.0 2.0 38.5 54-55 22.743440743806914 32.0 2.0 35.0 2.0 37.0 56-57 22.30042826211552 31.0 2.0 35.0 2.0 37.0 58-59 21.880810991530282 31.0 2.0 35.0 2.0 36.0 60-61 21.536171432210406 31.0 2.0 35.0 2.0 36.0 62-63 21.13145577278227 30.5 2.0 35.0 2.0 35.5 64-65 20.73010730433675 30.0 2.0 35.0 2.0 35.0 66-67 20.335652423103866 29.0 2.0 34.5 2.0 35.0 68-69 19.922634210023563 29.0 2.0 34.0 2.0 35.0 70-71 19.476278418136662 29.0 2.0 34.0 2.0 35.0 72-73 19.028290772463862 27.0 2.0 34.0 2.0 35.0 74-75 18.529206202636438 25.5 2.0 34.0 2.0 35.0 76-77 17.230282430108897 21.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0541476831041763 1101 2 1.0355789207887867 1101 3 0.7685184905190994 1101 4 0.16286694715306993 1101 5 1.0784719313301778 1101 6 0.0021136009986761906 1101 7 -0.7896189564031246 1101 8 -0.42992877981645705 1101 9 0.11427893746351714 1101 10-11 0.2654593781239427 1101 12-13 -0.6614687727821469 1101 14-15 -0.2034105181318786 1101 16-17 0.18056692851069656 1101 18-19 0.21740347039384034 1101 20-21 -2.243614085119262 1101 22-23 -2.142703643086083 1101 24-25 -1.665120588894304 1101 26-27 -1.8705707251857682 1101 28-29 -2.926327790471582 1101 30-31 -2.427234818056167 1101 32-33 -2.7495374385987468 1101 34-35 -2.5551076852303254 1101 36-37 -2.5598753256989895 1101 38-39 -3.230271871909604 1101 40-41 -3.068974922792094 1101 42-43 -3.5109974971757545 1101 44-45 -3.375734011156677 1101 46-47 -3.804987945283976 1101 48-49 -3.964623983757054 1101 50-51 -5.046300898719828 1101 52-53 -5.03614952973091 1101 54-55 -5.395333997365125 1101 56-57 -6.6293845768483415 1101 58-59 -6.402004912775876 1101 60-61 -6.311930384501032 1101 62-63 -6.092309941301014 1101 64-65 -6.045786290749303 1101 66-67 -5.202177720421911 1101 68-69 -5.011481468133109 1101 70-71 -5.37826473363527 1101 72-73 -5.756268802826016 1101 74-75 -6.2672302555979975 1101 76-77 -5.388456843093429 1102 1 2.493962497918993 1102 2 1.823277333487198 1102 3 1.9682539402545487 1102 4 2.4221262064123295 1102 5 2.7729163757746207 1102 6 1.8156056644907395 1102 7 1.0298254880413182 1102 8 0.5145156646279858 1102 9 1.3252577734423525 1102 10-11 1.616319166483727 1102 12-13 2.1549994811861026 1102 14-15 1.9758222861009251 1102 16-17 1.5949187803625477 1102 18-19 2.142337332827694 1102 20-21 2.4789388249336497 1102 22-23 2.0699286320461887 1102 24-25 2.3791915804178636 1102 26-27 1.867193825078779 1102 28-29 1.7581960190522246 1102 30-31 1.4203180919967444 1102 32-33 2.44027737621607 1102 34-35 1.8274981348754942 1102 36-37 2.3462093303856655 1102 38-39 3.1040535249157912 1102 40-41 2.3711705798534126 1102 42-43 2.8977326615544037 1102 44-45 3.185112549689883 1102 46-47 2.6272210494250174 1102 48-49 3.0443045876715153 1102 50-51 3.705352540433612 1102 52-53 3.668876925295546 1102 54-55 3.882774468243344 1102 56-57 3.772731825268064 1102 58-59 3.973325775054814 1102 60-61 4.410953213382566 1102 62-63 4.2413540798630045 1102 64-65 3.416184608721597 1102 66-67 4.168192649948459 1102 68-69 4.0073677382161 1102 70-71 4.676629446258911 1102 72-73 5.156760297703084 1102 74-75 5.183827945460202 1102 76-77 4.941569611933026 1103 1 -1.1502682713117736 1103 2 -1.0242501390402694 1103 3 -0.7927350707344587 1103 4 -1.6547968705107472 1103 5 -1.323237470379226 1103 6 -0.33549323660815844 1103 7 -0.21055912734329496 1103 8 -0.9277920276797076 1103 9 -1.689852116667538 1103 10-11 -1.535466547801981 1103 12-13 -2.059973046286423 1103 14-15 -2.7041227688441296 1103 16-17 -1.2175812196374523 1103 18-19 -1.317140689150321 1103 20-21 -0.4832864497916276 1103 22-23 -0.5063076316900741 1103 24-25 -0.60707215584587 1103 26-27 0.20991360529855996 1103 28-29 -1.1346611238049178 1103 30-31 -1.330368721190073 1103 32-33 -0.5308764699377768 1103 34-35 -0.3517601068827467 1103 36-37 -0.3529664937901593 1103 38-39 -0.6576222992600336 1103 40-41 -0.7352854641026347 1103 42-43 -0.4223222835005451 1103 44-45 -0.7874149228375913 1103 46-47 -0.2127514780475117 1103 48-49 -0.714623983757054 1103 50-51 -1.3942353716543003 1103 52-53 -2.256947250528629 1103 54-55 -1.9832969603280866 1103 56-57 -2.0720483945121586 1103 58-59 -2.4023610381319997 1103 60-61 -1.6673434899141384 1103 62-63 -1.6247173487084225 1103 64-65 -1.9086780286410416 1103 66-67 -2.351038119282311 1103 68-69 -2.3971652288168706 1103 70-71 -1.9373815427520782 1103 72-73 -1.7779924495496608 1103 74-75 -1.3202929336606744 1103 76-77 -1.0900237946603824 1104 1 1.525212497918993 1104 2 1.9973844763443438 1104 3 1.8611110831116946 1104 4 1.2971262064123295 1104 5 1.5697913757746207 1104 6 0.7798913787764548 1104 7 1.2017004880413182 1104 8 1.0145156646279858 1104 9 0.9123113448709219 1104 10-11 1.620783452198019 1104 12-13 1.7152673383289638 1104 14-15 0.8932330003866404 1104 16-17 1.9152312803625477 1104 18-19 1.9358641185419856 1104 20-21 1.730054896362219 1104 22-23 1.5766250606176193 1104 24-25 1.6771826518464366 1104 26-27 0.8102741822216366 1104 28-29 0.6075263761950822 1104 30-31 0.9448716634253138 1104 32-33 1.6277773762160699 1104 34-35 1.7281677777326365 1104 36-37 1.2870575446713808 1104 38-39 0.9667767392015065 1104 40-41 1.536349151281982 1104 42-43 1.9881344472686884 1104 44-45 1.7264071925470255 1104 46-47 1.992176406567875 1104 48-49 1.543188516242946 1104 50-51 2.1328078975764697 1104 52-53 2.5639662110098307 1104 54-55 2.3604530396719134 1104 56-57 2.348624682410918 1104 58-59 2.2947543464833835 1104 60-61 3.1788103562397083 1104 62-63 2.7268451512915775 1104 64-65 3.5847113944358817 1104 66-67 3.394755149948459 1104 68-69 2.1178588096446695 1104 70-71 2.5493973034017685 1104 72-73 3.339796011988799 1104 74-75 3.6983368740316322 1104 76-77 3.641346397647311 1105 1 -0.31285568389918694 1105 2 0.05477083998070498 1105 3 0.03383835583896655 1105 4 0.1857625700486949 1105 5 -0.45890180604356345 1105 6 0.5071641060491814 1105 7 -0.2633563301405033 1105 8 -0.6673025171901976 1105 9 -0.2055863824018047 1105 10-11 -0.9831938205292516 1105 12-13 -0.2958122071255822 1105 14-15 -0.4212556359769941 1105 16-17 -0.8903084923647242 1105 18-19 -0.42535747236710364 1105 20-21 -1.1575871490923255 1105 22-23 -1.134454484836926 1105 24-25 -1.0471071208808347 1105 26-27 -0.8767996814147274 1105 28-29 -0.25511566925946383 1105 30-31 0.3678830270616764 1105 32-33 0.1652773762160713 1105 34-35 0.2624007322780919 1105 36-37 0.8377677719441081 1105 38-39 0.8308392392015094 1105 40-41 0.8869173331001647 1105 42-43 0.8879924018141416 1105 44-45 0.246151510728847 1105 46-47 0.28777299747696716 1105 48-49 -0.07826034739341736 1105 50-51 0.47726812484919634 1105 52-53 0.2682275746461933 1105 54-55 -0.49466059669172324 1105 56-57 -0.9155798630436287 1105 58-59 -0.9382001989711632 1105 60-61 -0.2984623710330183 1105 62-63 -0.5497173487084197 1105 64-65 -0.2696920146550248 1105 66-67 -0.5318073500515403 1105 68-69 -0.8473400539916973 1105 70-71 -0.35486406023459693 1105 72-73 -0.18236307892029302 1105 74-75 -1.5194188077865505 1105 76-77 -0.6886251932617782 1106 1 1.3497317286882264 1106 2 1.0911344763443438 1106 3 1.6495726215732347 1106 4 1.941356975643096 1106 5 1.8690702219284674 1106 6 1.4721990710841482 1106 7 0.8086716418874715 1106 8 1.5722079723202924 1106 9 1.021686344870922 1106 10-11 1.3779949906595519 1106 12-13 1.8727192614058836 1106 14-15 2.4497233850020272 1106 16-17 3.311264934208701 1106 18-19 2.0290131570035186 1106 20-21 2.084021242516066 1106 22-23 2.820615445233006 1106 24-25 3.30638938261567 1106 26-27 2.94068283606779 1106 28-29 3.4326465685027756 1106 30-31 4.169631278809927 1106 32-33 3.7095081454468364 1106 34-35 3.01362450850187 1106 36-37 2.233571967748304 1106 38-39 3.3231469315092 1106 40-41 2.9089453051281353 1106 42-43 3.0007546395763818 1106 44-45 2.847200461777792 1106 46-47 3.566094675798645 1106 48-49 3.698837554704486 1106 50-51 3.4903800129610865 1106 52-53 3.4161296725482906 1106 54-55 3.8532415012103733 1106 56-57 3.8510285285647647 1106 58-59 3.7803312695603033 1106 60-61 3.0441949716243215 1106 62-63 3.077205728214654 1106 64-65 3.302860432897422 1106 66-67 2.812423419179229 1106 68-69 3.6605270788754396 1106 70-71 2.735695380324845 1106 72-73 2.683546011988799 1106 74-75 1.9297070663393256 1106 76-77 3.227283897647311 1107 1 0.27521249791899294 1107 2 0.1067594763443438 1107 3 0.4548610831116946 1107 4 0.5471262064123295 1107 5 0.1479163757746207 1107 6 -0.04823362122354524 1107 7 -0.6889245119586818 1107 8 -0.2667343353720142 1107 9 0.4123113448709219 1107 10-11 -0.004216547801981108 1107 12-13 -0.7456701616710362 1107 14-15 -1.4661419996133596 1107 16-17 -1.2332062196374523 1107 18-19 -0.9313233814580144 1107 20-21 -1.012132603637781 1107 22-23 -0.9468124393823807 1107 24-25 -1.1821923481535634 1107 26-27 -1.2834758177783634 1107 28-29 -1.5565361238049178 1107 30-31 -1.7426283365746862 1107 32-33 -1.6690976237839301 1107 34-35 -1.5530822222673635 1107 36-37 -0.9160674553286192 1107 38-39 -1.4004107607984935 1107 40-41 -1.526150848718018 1107 42-43 -1.0978030527313116 1107 44-45 -1.0860928074529745 1107 46-47 -0.6406360934321249 1107 48-49 -0.12868648375705405 1107 50-51 -0.9531296024235303 1107 52-53 -1.0532212889901693 1107 54-55 -0.6942344603280866 1107 56-57 -1.143562817589082 1107 58-59 -1.1114956535166165 1107 60-61 -1.4149396437602917 1107 62-63 -1.2028423487084225 1107 64-65 -1.7590386055641183 1107 66-67 -2.597432350051541 1107 68-69 -2.8821411903553305 1107 70-71 -2.5990401965982315 1107 72-73 -1.988328988011201 1107 74-75 -1.7391631259683678 1107 76-77 -1.428966102352689 1108 1 0.9673231536567002 1108 2 0.30424923044270713 1108 3 0.8529143618002166 1108 4 0.04507702608446351 1108 5 -0.263968870127016 1108 6 0.30448154271088157 1108 7 0.4663008978773817 1108 8 -0.15761548291299476 1108 9 0.37414536126436104 1108 10-11 0.7288777144931018 1108 12-13 0.5152161088207663 1108 14-15 0.9052719348128697 1108 16-17 1.5529105836412356 1108 18-19 0.8737483398534636 1108 20-21 1.2091788717720533 1108 22-23 1.1798525196340144 1108 24-25 1.7354562174202073 1108 26-27 1.0901153707462292 1108 28-29 1.3509946138999993 1108 30-31 1.4599843683433456 1108 32-33 1.3603593434291845 1108 34-35 1.096379867896573 1108 36-37 1.097380290573021 1108 38-39 0.950511370349048 1108 40-41 0.21821390538034535 1108 42-43 0.5891846521867201 1108 44-45 0.3115762499240766 1108 46-47 0.7722737426334483 1108 48-49 1.3017694588658948 1108 50-51 1.725405233642043 1108 52-53 1.79475514543606 1108 54-55 1.8794079577047 1108 56-57 1.9249566496240327 1108 58-59 1.8941395923850237 1108 60-61 2.2976628152561034 1108 62-63 1.5863482250620677 1108 64-65 2.24595627148506 1108 66-67 2.4960615024074784 1108 68-69 2.311378276857784 1108 70-71 2.6874608279919343 1108 72-73 1.6528083070707673 1108 74-75 2.501743636326715 1108 76-77 2.315398651745671 1109 1 -0.4008787719222795 1109 2 0.02764241285228053 1109 3 0.011904733905346632 1109 4 -0.6096198253337022 1109 5 -0.5405756877174426 1109 6 -0.013759414874336073 1109 7 0.05958739280322334 1109 8 -0.31088116076883665 1109 9 0.22009904328361785 1109 10-11 -0.46602210335753824 1109 12-13 0.10043598912261587 1109 14-15 0.8468540321326721 1109 16-17 1.0284505263942947 1109 18-19 1.2782500312403968 1109 20-21 1.2973912058860293 1109 22-23 0.2157619653795244 1109 24-25 0.4367312629575508 1109 26-27 0.42969382507877896 1109 28-29 0.9903388761950822 1109 30-31 0.6217069808856301 1109 32-33 -0.06964325870456278 1109 34-35 -0.4334145635372053 1109 36-37 -0.6418859077095718 1109 38-39 -0.5675734592111894 1109 40-41 -0.28855164236881237 1109 42-43 -0.43163241781067896 1109 44-45 -1.299014434437101 1109 46-47 -1.1475805378765678 1109 48-49 -0.5479573170903862 1109 50-51 -0.3849252373441665 1109 52-53 -0.6604881540695366 1109 54-55 -0.2233763254074539 1109 56-57 0.11400166653790222 1109 58-59 0.7104289496579845 1109 60-61 0.611350038779392 1109 62-63 0.2899651909741152 1109 64-65 -0.07488681984983359 1109 66-67 0.6364466182024273 1109 68-69 0.6512169445653022 1109 70-71 0.8135342081636736 1109 72-73 0.9672761707189572 1109 74-75 1.7671612787935373 1109 76-77 1.951490246853659 1110 1 -1.0564106364093675 1110 2 -0.4163282102228223 1110 3 -1.6239635437539803 1110 4 -1.5330976741846882 1110 5 -1.3726060122850825 1110 6 -0.5932429495817573 1110 7 -0.6809954074810705 1110 8 -0.2616037383570884 1110 9 -0.23950768497982722 1110 10-11 -0.2462874433243698 1110 12-13 -0.03543228853670399 1110 14-15 -0.7893696115536564 1110 16-17 -0.5981782345628268 1110 18-19 -0.5299707695177176 1110 20-21 -0.6986997678168869 1110 22-23 -0.6442238199793984 1110 24-25 -0.5549768630789345 1110 26-27 -0.1534618252410489 1110 28-29 -0.3454820193273065 1110 30-31 -0.21814139627617735 1110 32-33 0.15482961502204162 1110 34-35 0.08695975534457645 1110 36-37 -0.27159450756742487 1110 38-39 -0.2870712085596878 1110 40-41 -0.5471396546881664 1110 42-43 -0.805359022880566 1110 44-45 -0.1869556805872996 1110 46-47 0.4237295782096666 1110 48-49 0.15851034460115443 1110 50-51 0.11566703936751566 1110 52-53 0.4210091214575904 1110 54-55 0.1640910993734046 1110 56-57 -0.37758800415624805 1110 58-59 -0.8106561012778108 1110 60-61 -0.6458165094319313 1110 62-63 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52-53 -1.3210498146311949 2314 54-55 -1.9352200372511632 2314 56-57 -1.9662791637429287 2314 58-59 -2.1299251406961055 2314 60-61 -2.4301640027346494 2314 62-63 -2.416384015375087 2314 64-65 -2.379831874794885 2314 66-67 -2.267704785948979 2314 68-69 -2.9131908698425093 2314 70-71 -2.174561029931567 2314 72-73 -2.1978642444214564 2314 74-75 -2.938882677250419 2314 76-77 -3.115664820301408 2315 1 -1.9013077723512808 2315 2 -1.1656222804124141 2315 3 -1.3280781060774913 2315 4 -0.983279198993074 2315 5 -0.9905971377388951 2315 6 -1.4092978104127347 2315 7 -1.5613907281748993 2315 8 -1.7557546056422844 2315 9 -1.0593947362101588 2315 10-11 -0.7145206018560337 2315 12-13 -1.753060364373738 2315 14-15 -1.6916487563701175 2315 16-17 -1.3865001385563716 2315 18-19 -1.1209348679445021 2315 20-21 -0.9580785495837247 2315 22-23 -1.330891831274272 2315 24-25 -1.5932987670724827 2315 26-27 -1.6050552096702546 2315 28-29 -1.651553015696809 2315 30-31 -1.8579154987368476 2315 32-33 -1.7252631643244705 2315 34-35 -2.375928506051146 2315 36-37 -2.2097752255988894 2315 38-39 -1.5651067067444373 2315 40-41 -2.0084143622315302 2315 42-43 -1.221958458136715 2315 44-45 -1.138246523669192 2315 46-47 -1.1220550123510442 2315 48-49 -0.964623983757054 2315 50-51 -1.2229775753965058 2315 52-53 -0.6400033835847658 2315 54-55 -0.6961347981659252 2315 56-57 -0.7349901824539451 2315 58-59 -1.0819348427058024 2315 60-61 -0.990108562679211 2315 62-63 -0.8916092406003138 2315 64-65 -0.691682186645199 2315 66-67 -0.5277532959974849 2315 68-69 -1.2201901768418146 2315 70-71 -0.14528175065228766 2315 72-73 -0.26577831233552374 2315 74-75 -0.5497627881305291 2315 76-77 -2.6139323185689065 2316 1 -1.83103750208101 2316 2 -1.7374369522270854 2316 3 -1.4960317740311595 2316 4 -4.449302365016241 2316 5 -3.4199407670825224 2316 6 -3.6486800497949723 2316 7 -2.0166030833872526 2316 8 -1.599770049657728 2316 9 -2.3354565122719357 2316 10-11 -1.8461808335162644 2316 12-13 -3.386071947385325 2316 14-15 -5.420606285327647 2316 16-17 -5.9890097910660245 2316 18-19 -4.698734095743731 2316 20-21 -4.897846889352067 2316 22-23 -4.617571367953808 2316 24-25 -5.010094133867849 2316 26-27 -5.184591889206935 2316 28-29 -5.002518266662062 2316 30-31 -5.774324765146115 2316 32-33 -5.44186548092679 2316 34-35 -5.413573293695936 2316 36-37 -5.532362098185761 2316 38-39 -6.219160760798495 2316 40-41 -5.459186563003733 2316 42-43 -5.852267338445596 2316 44-45 -5.236316021738691 2316 46-47 -5.553136093432126 2316 48-49 -5.907481126614197 2316 50-51 -5.092861745280672 2316 52-53 -5.888265931847311 2316 54-55 -5.444011246042372 2316 56-57 -5.555839603303369 2316 58-59 -5.799888510659475 2316 60-61 -6.0640467866174355 2316 62-63 -6.20686020585128 2316 64-65 -5.724886819849832 2316 66-67 -6.274664492908684 2316 68-69 -6.140846547498187 2316 70-71 -6.7697991251696585 2316 72-73 -6.4128825594397725 2316 74-75 -6.109029197396939 2316 76-77 -5.547716102352689 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5842.0 3 365.0 4 599.0 5 738.0 6 733.0 7 757.0 8 1031.0 9 1103.0 10 1369.0 11 1336.0 12 975.0 13 677.0 14 638.0 15 676.0 16 672.0 17 669.0 18 614.0 19 751.0 20 629.0 21 678.0 22 652.0 23 747.0 24 731.0 25 834.0 26 885.0 27 1049.0 28 1104.0 29 1306.0 30 1531.0 31 1729.0 32 2055.0 33 2533.0 34 3653.0 35 4259.0 36 5229.0 37 6300.0 38 5578.0 39 1776.0 40 9.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 18.448580572229208 29.668667505214387 34.06785867817281 17.814893244383587 2 17.876534560450935 29.74539432829642 34.45854497396621 17.919526137286436 3 17.754494355185425 29.90239008932979 33.808378847470586 18.534736708014204 4 17.882735118952834 29.841714704289945 34.75906876015159 17.516481416605625 5 18.218442968837085 30.111944457714294 33.82537938502205 17.844233188426568 6 18.04140127388535 30.823248407643312 34.12738853503185 17.00796178343949 7 18.170671507508082 30.322138887561906 33.076959824201026 18.430229780728993 8 17.070607344182935 30.743017293544376 34.02019172585114 18.166183636421543 9 17.966846605837674 30.556219048074013 34.02124237647096 17.45569196961735 10-11 17.696282554519616 30.902011990732266 33.32802535092399 18.073680103824135 12-13 17.68424740429327 30.855468501178418 33.23858207529142 18.221702019236893 14-15 18.00724493451694 30.094343378050237 33.72954898292265 18.168862704510172 16-17 18.223722444690356 30.47185357970241 33.253457101686955 18.050966873920277 18-19 18.214388076812842 30.331040412725706 32.73621859176459 18.71835291869686 20-21 18.254846542733173 30.00437880657617 33.15871183471996 18.5820628159707 22-23 18.34749170003901 30.266792990613283 32.421996289897535 18.963719019450174 24-25 18.490914592390872 30.137117194591756 31.759113276957624 19.61285493605975 26-27 18.572895849423155 29.80883301352739 31.442629999124183 20.17564113792527 28-29 19.108503371573693 29.860917609406968 31.193624660653295 19.83695435836604 30-31 18.411101115365938 30.030491445676667 31.19601302454442 20.36239441441298 32-33 18.462346843726863 29.529405208308457 31.22994737554436 20.778300572420328 34-35 18.045603643196078 29.499060539473266 31.580204452087514 20.875131365243146 36-37 17.97764438004554 29.063102081110774 32.06136649523112 20.897887043612567 38-39 17.896270581865664 28.929265263224945 32.24067645466416 20.93378770024523 40-41 17.641719745222932 28.868630573248407 32.08200636942675 21.40764331210191 42-43 17.840678370954258 28.807675464787614 31.55061905330626 21.801027110951868 44-45 18.242328699501584 28.268762241436967 31.717861749390913 21.771047309670536 46-47 18.094669373780803 28.49317249890521 31.3873959950635 22.02476213225049 48-49 18.049440706978224 28.29505194856893 31.541737988137413 22.113769356315434 50-51 18.57650225357945 28.1617799296055 30.78086926053927 22.48084855627578 52-53 18.43651943997579 27.74556806778905 31.082458627335424 22.735453864899736 54-55 18.847746456442106 27.6644370122631 30.969103360407708 22.518713170887082 56-57 18.77592617658065 27.53568954672484 30.651209821890653 23.037174454803857 58-59 18.661369047145097 27.33794619041173 30.65824780438089 23.342436958062283 60-61 18.621002675500065 27.135622372276725 30.705344629889158 23.538030322334055 62-63 19.150528729774493 27.372913746974138 29.708402344247673 23.768155179003696 64-65 18.7803071925089 26.854262714091202 30.0448287668506 24.320601326549298 66-67 18.98608191604561 26.4212688706287 30.25670424867826 24.335944964647428 68-69 19.02778552031154 26.178435760418573 30.278173753075997 24.515604966193884 70-71 19.182545245985217 25.548049961763958 30.670883252612796 24.598521539638032 72-73 19.45281004509457 25.194002262695797 30.437242060646618 24.915945631563012 74-75 19.3764540905759 25.277273161870163 30.432323039168818 24.913949708385122 76-77 19.472396841711085 25.083857191799925 30.44593700950514 24.99780895698385 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2308.0 1 1312.5 2 291.5 3 232.5 4 199.0 5 222.0 6 238.5 7 256.5 8 263.5 9 246.0 10 255.5 11 266.5 12 266.5 13 265.0 14 275.0 15 270.5 16 286.5 17 317.0 18 351.0 19 424.0 20 499.5 21 584.0 22 632.0 23 670.5 24 758.0 25 904.5 26 1002.0 27 1030.5 28 1209.5 29 1427.0 30 1494.0 31 1570.5 32 1802.5 33 1975.0 34 2026.0 35 2060.0 36 2115.0 37 2074.5 38 1976.5 39 1974.0 40 2085.0 41 2269.5 42 2287.0 43 2231.0 44 2300.5 45 2335.0 46 2320.0 47 2325.0 48 2310.0 49 2392.0 50 2468.5 51 2217.0 52 1971.0 53 1966.5 54 1847.5 55 1536.5 56 1307.5 57 1275.0 58 1175.0 59 992.5 60 867.5 61 825.0 62 753.0 63 580.5 64 432.0 65 384.0 66 339.5 67 245.0 68 180.5 69 147.0 70 128.0 71 105.5 72 76.0 73 65.0 74 61.0 75 48.5 76 43.0 77 36.0 78 22.0 79 16.5 80 12.0 81 13.5 82 11.5 83 9.0 84 6.5 85 4.5 86 3.5 87 2.0 88 3.5 89 2.5 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007960262370247724 2 0.014328472266445902 3 0.01751257721454499 4 0.022288734636693626 5 0.020696682162644083 6 0.019104629688594534 7 0.020696682162644083 8 0.022288734636693626 9 0.020696682162644083 10-11 0.021492708399668853 12-13 0.025472839584792715 14-15 0.015124498503470675 16-17 0.010348341081322042 18-19 0.015920524740495448 20-21 0.015124498503470675 22-23 0.016716550977520218 24-25 0.03024899700694135 26-27 0.021492708399668853 28-29 0.011940393555371584 30-31 0.011940393555371584 32-33 0.01353244602942113 34-35 0.015920524740495448 36-37 0.014328472266445902 38-39 0.022288734636693626 40-41 0.019104629688594534 42-43 0.0230847608737184 44-45 0.020696682162644083 46-47 0.0230847608737184 48-49 0.015124498503470675 50-51 0.03661720690313953 52-53 0.0461695217474368 54-55 0.03502515442908998 56-57 0.021492708399668853 58-59 0.026268865821817484 60-61 0.031841049480990896 62-63 0.031841049480990896 64-65 0.02786091829586703 66-67 0.025472839584792715 68-69 0.04378144303636248 70-71 0.07005030885817996 72-73 0.08756288607272496 74-75 0.0923390434948736 76-77 0.08995096478379927 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 62812.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.55358848627651 #Duplication Level Percentage of deduplicated Percentage of total 1 86.04422266984069 59.84684455199643 2 7.15986083134957 9.959880277653951 3 2.691814685954953 5.616761128446793 4 1.4740889946896172 4.101127173151627 5 0.8492034425929317 2.9532573393619055 6 0.46923640358908625 1.95822454308094 7 0.35478850027467496 1.7273769343437562 8 0.233473722761399 1.2991148188244284 9 0.1442043581761582 0.9026937527860919 >10 0.5653726423731917 5.807807425332738 >50 0.009155832265152902 0.37254027892759345 >100 0.0 0.0 >500 0.0 0.0 >1k 0.004577916132576451 5.4543717760937405 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1882 2.996242756161243 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1544 2.458129019932497 No Hit TTAAAAACAAAAATGAAGTATTAAGGCGGAAGATTTAAAAAAATTTTTGC 68 0.10825956823536904 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006368209896198179 0.0 2 0.0 0.0 0.0 0.02388078711074317 0.0 3 0.0 0.0 0.0 0.03024899700694135 0.0 4 0.0 0.0 0.0 0.049353626695535883 0.0 5 0.0 0.0 0.0 0.08119467617652677 0.0 6 0.0 0.0 0.0 0.11781188307966631 0.0 7 0.0 0.0 0.0 0.18945424441189582 0.0 8 0.0 0.0 0.0 0.24836018595172896 0.0 9 0.0 0.0 0.0 0.33751512449850346 0.0 10 0.0 0.0 0.0 0.4553270075781698 0.0 11 0.0 0.0 0.0 0.6049799401388269 0.0 12 0.0 0.0 0.0 0.748264662803286 0.0 13 0.0 0.0 0.0 0.9361268547411322 0.0 14 0.0 0.0 0.0 1.101700312042285 0.0 15 0.0 0.0 0.0 1.2672737693434375 0.0 16 0.0 0.0 0.0 1.4232949118002929 0.0 17 0.0 0.0 0.0 1.5458829523021078 0.0 18 0.0 0.0 0.0 1.6732471502260715 0.0 19 0.0 0.0 0.0 1.8069795580462331 0.0 20 0.0 0.0 0.0 1.9502642807106922 0.0 21 0.0 0.0 0.0 2.1031013182194487 0.0 22 0.0 0.0 0.0 2.2320575686174617 0.0 23 0.0 0.0 0.0 2.3450932942749794 0.0 24 0.0 0.0 0.0 2.4660892823027445 0.0 25 0.0 0.0 0.0 2.5759409030121634 0.0 26 0.0 0.0 0.0 2.7144494682544735 0.0 27 0.0 0.0 0.0 2.825893141437942 0.0 28 0.0 0.0 0.0 2.934152709673311 0.0 29 0.0 0.0 0.0 3.0615169075972744 0.0 30 0.0 0.0 0.0 3.236642679742724 0.0 31 0.0 0.0 0.0 3.4038081895179264 0.0 32 0.0 0.0 0.0 3.612367063618417 0.0 33 0.0 0.0 0.0 3.81137362287461 0.0 34 0.0 0.0 0.0 4.008788129656754 0.0 35 0.0 0.0 0.0 4.185505954276253 0.0 36 0.0 0.0 0.0 4.33675093931096 0.0 37 0.0 0.0 0.0 4.519836973826657 0.0 38 0.0 0.0 0.0 4.679042221231612 0.0 39 0.0 0.0 0.0 4.843023626058715 0.0 40 0.0 0.0 0.0 5.056358657581354 0.0 41 0.0 0.0 0.0 5.268101636629943 0.0 42 0.0 0.0 0.0 5.514869770107623 0.0 43 0.0 0.0 0.0 5.783926638221995 0.0 44 0.0 0.0 0.0 6.038655034069923 0.0 45 0.0 0.0 0.0 6.298159587339999 0.0 46 0.0 0.0 0.0 6.595873399987264 0.0 47 0.0 0.0 0.0 6.8283130611984975 0.0 48 0.0 0.0 0.0 7.156275870852704 0.0 49 0.0 0.0 0.0 7.430108896389225 0.0 50 0.0 0.0 0.0 7.700757816977648 0.0 51 0.0 0.0 0.0 7.976182894988219 0.0 52 0.0 0.0 0.0 8.28822517990193 0.0 53 0.0 0.0 0.0 8.614595937082086 0.0 54 0.0 0.0 0.0 8.968031586321086 0.0 55 0.0 0.0 0.0 9.30077055339744 0.0 56 0.0 0.0 0.0 9.658982360058587 0.0 57 0.0 0.0 0.0 10.039482901356429 0.0 58 0.0 0.0 0.0 10.442272177290963 0.0 59 0.0 0.0 0.0 10.8418773482774 0.0 60 0.0 0.0 0.0 11.21919378462714 0.0 61 0.0 0.0 0.0 11.617206903139527 0.0 62 0.0 0.0 0.0 12.039100808762656 0.0 63 0.0 0.0 0.0 12.460994714385786 0.0 64 0.0 0.0 0.0 12.895625039801311 0.0 65 0.0 0.0 0.0 13.32547920779469 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTAC 15 0.0020944795 70.995224 34 CTCGTTA 15 0.0020944795 70.995224 33 GCTGCCT 15 0.0020944795 70.995224 28 TACGGAT 15 0.0020944795 70.995224 38 CGTTACG 15 0.0020944795 70.995224 35 CGGATGG 15 0.0020944795 70.995224 40 ACGGATG 15 0.0020944795 70.995224 39 TTACGGA 15 0.0020944795 70.995224 37 CTTGTTG 20 0.006525158 53.28884 68 GTAGTCG 20 0.006525158 53.28884 68 TACTTTA 20 0.0065457704 53.246418 22 AGTCAGT 20 0.0065457704 53.246418 5 CAAGTCA 20 0.0065457704 53.246418 3 GGCGTCA 20 0.0065457704 53.246418 23 GTTACGG 20 0.0065457704 53.246418 36 CGTCAGA 20 0.0065457704 53.246418 25 TGCCTCA 50 0.006682518 28.398088 30 GTCGCCG 95 0.0065481956 18.697842 71 AGCAGTG 205 0.0029566332 12.121135 1 GGTATCA 225 0.005925931 11.043701 7 >>END_MODULE