FastQCFastQC Report
Thu 26 May 2016
SRR936088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62812
Sequences flagged as poor quality0
Sequence length125
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT10771.7146405145513597No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9571.5235942176654143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8481.3500604979940138No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7981.2704578742915367No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT7241.1526459912118703TruSeq Adapter, Index 3 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5620.894733490415844No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG4500.7164236133222951TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT4180.6654779341527097RNA PCR Primer, Index 3 (95% over 23bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.6320448321976692No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.35821180666114755No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCT2080.33114691460230533TruSeq Adapter, Index 3 (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.30885817996561166No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.24676813347767942No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.2419919760555308No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTG1280.20378271667834172TruSeq Adapter, Index 3 (95% over 24bp)
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC1090.17353371967140038No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTGA1040.16557345730115267RNA PCR Primer, Index 3 (96% over 28bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.16238935235305355No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.14328472266445902No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA820.13054830287206268No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.12418009297586448No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA770.12258804050181493No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA710.11303572565751767No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT660.10507546328726994No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT650.1034834108132204No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.10189135833917086No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGC152.6896875E-4119.000011
CTGCTCA250.002047776571.49
CAATACG250.002047776571.49
CGAGTAC351.3287718E-859.500004100-101
TCTTATG257.4135532E-659.50000496-97
CCCGTAT150.00422778359.50000498-99
CCCGTAC150.00422778359.50000498-99
CCGAGTA150.00422778359.500004100-101
CCGAGCG150.00422778359.50000496-97
TCGTACA150.00422778359.500004100-101
CGAGCGT150.00422778359.50000496-97
TTACGCA351.3287718E-859.500004100-101
CAATGGG300.00421795259.5000043
AATAGCG150.00422778359.50000496-97
GGTTCGG150.00422778359.50000422-23
CCCGGTA257.4135532E-659.50000498-99
TCGACAC150.00422778359.50000462-63
TGCACGC303.1304626E-759.50000498-99
GTCGGTA150.00422778359.50000498-99
AACGGTA201.7658807E-459.49999698-99
Produced by FastQC (version 0.11.5)