FastQCFastQC Report
Thu 26 May 2016
SRR936087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5442412
Sequences flagged as poor quality0
Sequence length77
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129930.23873606040851003No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG7500.021.77239856
GACGTAT9700.021.5900739
CGCGGAA8100.020.5980759
TCGCGCT8300.020.10062237
CGGTTAC6800.019.8353774
CTCGCGC8350.019.5537136
CGATCGT9000.018.9304311
TGCGTAA10050.018.71972837
AATTCGC9000.018.14316455
CAATTCG9100.017.94560454
TTGCGTA10400.017.74711636
CGGAGCG6900.017.49055535
CGCGCTT9350.017.46288538
ACGTATT12050.017.38019840
GCGTAAG10650.017.33099638
CGTAAGT10750.016.50955239
TCGCGGA10350.016.11993258
CCTCGCG10500.015.88847835
ATCGTTC11300.015.7054133
CGCTCCG10900.015.30541625
Produced by FastQC (version 0.11.5)