##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936087_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5442412 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.09185210527979 39.0 36.0 41.0 28.0 41.0 2 35.94767026090638 39.0 35.0 41.0 27.0 41.0 3 35.84841151313058 39.0 35.0 41.0 27.0 41.0 4 35.722675166819414 39.0 35.0 41.0 27.0 41.0 5 35.649464061155236 39.0 35.0 40.0 27.0 41.0 6 35.550121710741486 39.0 35.0 40.0 27.0 41.0 7 35.379656483191646 38.0 35.0 40.0 27.0 41.0 8 35.1938500797073 38.0 34.0 40.0 26.0 41.0 9 35.099540240613905 38.0 34.0 40.0 25.0 41.0 10-11 35.30758228153253 38.0 35.0 40.0 26.0 41.0 12-13 35.4908790440709 39.0 35.0 40.5 27.0 41.0 14-15 35.436831592316054 39.0 35.0 41.0 26.0 41.0 16-17 35.26168388207287 39.0 35.0 41.0 25.5 41.0 18-19 35.10431064388363 39.0 34.5 41.0 25.0 41.0 20-21 34.892300693148556 39.0 34.0 41.0 24.0 41.0 22-23 34.61966752976437 38.5 34.0 40.5 23.5 41.0 24-25 34.38297559978921 38.0 33.5 40.0 22.5 41.0 26-27 33.458771864386605 37.5 32.0 39.5 21.0 40.5 28-29 33.49123541179904 38.0 33.0 39.5 20.0 40.5 30-31 33.85674467497132 38.0 33.0 40.0 20.5 41.0 32-33 33.691231204105826 38.0 33.0 40.0 19.0 41.0 34-35 33.41246767058429 38.0 33.0 40.0 17.5 41.0 36-37 33.113455026925564 37.0 32.0 40.0 15.0 41.0 38-39 32.82646628002437 37.0 32.0 40.0 13.0 41.0 40-41 32.438965572617434 36.0 31.5 39.5 9.0 41.0 42-43 32.07085286817683 36.0 31.0 39.0 8.0 41.0 44-45 31.6931773081494 35.0 31.0 39.0 6.5 40.5 46-47 31.297956861773784 35.0 31.0 38.5 2.0 40.0 48-49 30.828582896700947 35.0 30.0 37.5 2.0 40.0 50-51 30.38420997895786 35.0 30.0 37.0 2.0 39.0 52-53 29.927035200569158 34.5 29.5 36.0 2.0 39.0 54-55 29.4610815020987 34.0 29.0 36.0 2.0 38.5 56-57 29.005474227236014 34.0 29.0 35.5 2.0 37.0 58-59 28.574329267978975 34.0 28.5 35.0 2.0 37.0 60-61 28.21140231573795 34.0 28.0 35.0 2.0 36.0 62-63 27.855296511914204 34.0 27.0 35.0 2.0 36.0 64-65 27.474030632006546 34.0 26.5 35.0 2.0 35.5 66-67 27.079548001878578 33.0 26.0 35.0 2.0 35.0 68-69 26.698594942830496 33.0 25.0 35.0 2.0 35.0 70-71 26.258943920452914 33.0 24.5 35.0 2.0 35.0 72-73 25.75383524804811 33.0 23.5 35.0 2.0 35.0 74-75 25.141158368752677 32.5 19.0 35.0 2.0 35.0 76-77 23.435112134105246 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2848656297140124 1101 2 1.258858853349743 1101 3 1.316656390576881 1101 4 1.2140530459558647 1101 5 1.205070128399953 1101 6 1.1333920392804089 1101 7 1.2343810537166604 1101 8 1.4268015988773541 1101 9 1.3811229443831436 1101 10-11 1.299202738975083 1101 12-13 1.3519353225739437 1101 14-15 1.4512977235585964 1101 16-17 1.465597751269165 1101 18-19 1.549993757786595 1101 20-21 1.5256993932422063 1101 22-23 1.4872683936191393 1101 24-25 1.5464363070487295 1101 26-27 1.4364904045856548 1101 28-29 1.4880641671648505 1101 30-31 1.519063276410023 1101 32-33 1.526582211007586 1101 34-35 1.484416099476725 1101 36-37 1.537993200335812 1101 38-39 1.629842861496158 1101 40-41 1.5837990714675172 1101 42-43 1.4489307530001199 1101 44-45 1.4248203934617614 1101 46-47 1.3660043649779148 1101 48-49 1.4976782664194026 1101 50-51 1.3994752421828842 1101 52-53 1.505177873989009 1101 54-55 1.4164124392473987 1101 56-57 1.339984985074274 1101 58-59 1.3546897706233878 1101 60-61 1.3183373002566583 1101 62-63 1.2457901175108788 1101 64-65 1.1849727168254205 1101 66-67 1.2296779787379641 1101 68-69 1.3209318716503482 1101 70-71 1.342755216379409 1101 72-73 1.4174726956113481 1101 74-75 1.4028238751574094 1101 76-77 1.3320556287858984 1102 1 1.1128118524688517 1102 2 1.2830789197817722 1102 3 1.208675860279726 1102 4 1.1616637922585227 1102 5 1.1855289515114649 1102 6 1.2024316538785484 1102 7 1.1068954430816689 1102 8 1.1532261291152608 1102 9 1.267216611624434 1102 10-11 1.1106817081323754 1102 12-13 1.2366938129212812 1102 14-15 1.1207089744363827 1102 16-17 1.1486051059054105 1102 18-19 1.304382221098912 1102 20-21 1.3001806203753645 1102 22-23 1.2733155092530737 1102 24-25 1.3599616701965687 1102 26-27 1.240038738657944 1102 28-29 1.298823110804328 1102 30-31 1.4046415970805555 1102 32-33 1.3915273262742218 1102 34-35 1.4382006142865862 1102 36-37 1.4714795533229719 1102 38-39 1.4844981228991756 1102 40-41 1.5159623276812653 1102 42-43 1.539250398741956 1102 44-45 1.4806471605065532 1102 46-47 1.4666267716169195 1102 48-49 1.5162030234040493 1102 50-51 1.5549662394011463 1102 52-53 1.4882448886014963 1102 54-55 1.5033430359348863 1102 56-57 1.4173164159803697 1102 58-59 1.469350679482762 1102 60-61 1.4137701446375246 1102 62-63 1.3809050073667812 1102 64-65 1.373440717377079 1102 66-67 1.3671619384231484 1102 68-69 1.417676595549846 1102 70-71 1.3847794030816978 1102 72-73 1.4935686825233994 1102 74-75 1.5145343583866762 1102 76-77 1.7169920851947147 1103 1 0.7433287494505976 1103 2 0.5472672256166291 1103 3 0.6486360680570797 1103 4 0.711358137692109 1103 5 0.8644301022447891 1103 6 0.7190813875827118 1103 7 0.7710698685762551 1103 8 0.8646369889097585 1103 9 0.7347142948851157 1103 10-11 0.8689532139038434 1103 12-13 0.8185424976908564 1103 14-15 0.7908345061093911 1103 16-17 0.7897406426195559 1103 18-19 0.9795057159360567 1103 20-21 1.0220275008442457 1103 22-23 0.9097080461371689 1103 24-25 0.8479721136095577 1103 26-27 0.7049723268995862 1103 28-29 0.7171330738743009 1103 30-31 1.1018561486723684 1103 32-33 1.0540569960398258 1103 34-35 1.1257116309992625 1103 36-37 1.1278951269786361 1103 38-39 1.1632637716550818 1103 40-41 1.1084390899533858 1103 42-43 1.0884155831165145 1103 44-45 1.031550868265537 1103 46-47 0.7763029764361953 1103 48-49 0.8172081423357405 1103 50-51 0.9253026890885714 1103 52-53 0.9591073342858714 1103 54-55 0.9572318135003535 1103 56-57 0.9494682074772776 1103 58-59 0.8617119062235012 1103 60-61 0.9169435320491637 1103 62-63 0.7564360703338657 1103 64-65 0.7530159904480129 1103 66-67 0.7814495453893286 1103 68-69 0.7553081214628783 1103 70-71 0.7879951420382838 1103 72-73 0.8603110283841566 1103 74-75 0.7110198332636912 1103 76-77 0.7602076346781992 1104 1 0.8969032705218751 1104 2 0.695555539630746 1104 3 0.7403500742799736 1104 4 0.7737491547113393 1104 5 1.0010633432040947 1104 6 0.6569293166362797 1104 7 0.8776752453918277 1104 8 0.8960413045506002 1104 9 0.8879167828494232 1104 10-11 0.8358615080269658 1104 12-13 0.9846265787350035 1104 14-15 0.8839984474678886 1104 16-17 0.9514142143816215 1104 18-19 0.9989578822763221 1104 20-21 0.8954145527289512 1104 22-23 0.9468007231340536 1104 24-25 1.004587508339526 1104 26-27 0.7009855200171415 1104 28-29 0.6955941319478143 1104 30-31 0.9004756041010964 1104 32-33 0.8749311843828664 1104 34-35 0.8746869247350233 1104 36-37 0.7350242965893798 1104 38-39 0.8782028817632508 1104 40-41 0.8962615979279533 1104 42-43 0.8911322128827948 1104 44-45 0.8423944512056316 1104 46-47 0.6862665981302136 1104 48-49 0.8800437708672142 1104 50-51 0.7922188853652905 1104 52-53 0.7962541193802402 1104 54-55 0.7795339273867867 1104 56-57 0.7707142869299197 1104 58-59 0.7223269869972846 1104 60-61 0.5158631592663099 1104 62-63 0.6872204645106059 1104 64-65 0.7536780886806547 1104 66-67 0.7040700315769612 1104 68-69 0.6531005806641907 1104 70-71 0.7328950331831052 1104 72-73 0.6458030222884936 1104 74-75 0.6905786729975105 1104 76-77 0.6726617659780558 1105 1 0.029411804625205207 1105 2 0.3153843200527007 1105 3 0.22823096457636183 1105 4 0.3310596388991911 1105 5 0.3715755348689811 1105 6 0.16728411146034716 1105 7 0.06129700477863764 1105 8 0.3919786580321585 1105 9 0.18786996790645816 1105 10-11 0.35869829488628824 1105 12-13 0.4319169798622511 1105 14-15 0.22278506233240591 1105 16-17 0.30505234214579247 1105 18-19 0.48407424286597944 1105 20-21 0.4807875173921019 1105 22-23 0.5428091771428498 1105 24-25 0.5060761312794853 1105 26-27 0.4103434173290381 1105 28-29 0.4967957273102357 1105 30-31 0.4692390917806364 1105 32-33 0.6654965808135458 1105 34-35 0.6602167434056199 1105 36-37 0.633031157932173 1105 38-39 0.5674141572992539 1105 40-41 0.7373934480725879 1105 42-43 0.6095942417775007 1105 44-45 0.48796958759251297 1105 46-47 0.38849883182387757 1105 48-49 0.30377337669745685 1105 50-51 0.4232483404617078 1105 52-53 0.3988759513222213 1105 54-55 0.4027214271761288 1105 56-57 0.4626655759728955 1105 58-59 0.38433079782943835 1105 60-61 0.1182874779172991 1105 62-63 0.14715732199148235 1105 64-65 0.3137553377714539 1105 66-67 0.3932929798043183 1105 68-69 0.03934741834938649 1105 70-71 -0.18107379008720415 1105 72-73 -0.21350295993034152 1105 74-75 -0.2968886350403821 1105 76-77 -0.043288399080541495 1106 1 0.0077764210895381325 1106 2 0.10617078201910601 1106 3 -0.2556179946791275 1106 4 0.045556324999154185 1106 5 -0.22586716960906728 1106 6 -0.09623415463149598 1106 7 0.09815826752351597 1106 8 0.1545450622461928 1106 9 0.09422248068955952 1106 10-11 -0.19050144145226966 1106 12-13 -0.1026408894859685 1106 14-15 -0.07829777699126339 1106 16-17 0.09771282329236186 1106 18-19 -0.022203430741356556 1106 20-21 -0.13888770191691435 1106 22-23 -0.025581083738337895 1106 24-25 0.08030663878509614 1106 26-27 -0.0660938681527341 1106 28-29 0.015971568442132877 1106 30-31 0.08297186721546979 1106 32-33 -0.026506613272708535 1106 34-35 -0.06604910525641827 1106 36-37 -0.09202551363369338 1106 38-39 -0.06517449957041066 1106 40-41 -0.09361736432177281 1106 42-43 -0.09905553150068869 1106 44-45 -0.3210133711183012 1106 46-47 -0.07088267660681424 1106 48-49 -0.22375837395618348 1106 50-51 -0.2121452033883635 1106 52-53 -0.23693726076097832 1106 54-55 -0.05917949499895059 1106 56-57 -0.15133679825995827 1106 58-59 -0.2887912543780864 1106 60-61 -0.3005773451102698 1106 62-63 -0.3812981858518789 1106 64-65 -0.34402310129310365 1106 66-67 -0.3286195575530506 1106 68-69 -0.41912985151748217 1106 70-71 -0.5689255253250458 1106 72-73 -0.5930823353200942 1106 74-75 -0.8394544561843489 1106 76-77 -0.59714759405988 1107 1 0.033562508502051 1107 2 0.15105764824378554 1107 3 0.08166894607487052 1107 4 0.142928014039029 1107 5 0.1485204366264199 1107 6 -0.04561256475595599 1107 7 0.058585697001589665 1107 8 0.11518042467861278 1107 9 0.13320846898576377 1107 10-11 0.14837693073522473 1107 12-13 0.2416573055791531 1107 14-15 0.23858604275152118 1107 16-17 0.150101963333519 1107 18-19 0.14000060094897293 1107 20-21 0.18345843970224252 1107 22-23 0.1590397291498462 1107 24-25 -0.08077465099469094 1107 26-27 -0.05809253123507574 1107 28-29 0.13636703002928385 1107 30-31 0.3487640869693891 1107 32-33 0.4521555278508558 1107 34-35 0.4889506018126255 1107 36-37 0.462239344928669 1107 38-39 0.466838651588688 1107 40-41 0.47228645732266017 1107 42-43 0.5162440120072418 1107 44-45 0.5050186582401075 1107 46-47 0.35766870300192366 1107 48-49 0.5229358282199534 1107 50-51 0.4086320288655436 1107 52-53 0.41287567161230854 1107 54-55 0.29889257754035725 1107 56-57 0.4115224623439069 1107 58-59 0.4705171068317071 1107 60-61 0.30595455891210577 1107 62-63 0.5000761837463088 1107 64-65 0.46865932025662715 1107 66-67 0.4900915990824082 1107 68-69 0.4245019256321747 1107 70-71 0.3706495423131919 1107 72-73 0.3447724832980228 1107 74-75 0.39637704993214484 1107 76-77 0.42354401591744306 1108 1 0.17947026757790496 1108 2 0.27435423804992354 1108 3 0.17722910458675756 1108 4 0.12489220547006852 1108 5 0.1980014767840359 1108 6 0.16609440540743492 1108 7 0.12357083133138502 1108 8 0.2389333868302117 1108 9 0.1928665958698801 1108 10-11 -0.0015367596891593394 1108 12-13 0.17677176750985524 1108 14-15 0.21921834970294896 1108 16-17 0.2765116822575564 1108 18-19 0.16957697700774332 1108 20-21 0.06053261720008152 1108 22-23 0.055796286083257485 1108 24-25 0.1913891722551 1108 26-27 0.0244984966470696 1108 28-29 0.20668202309584416 1108 30-31 0.26854569796542904 1108 32-33 0.29799167310424934 1108 34-35 0.3001914853481651 1108 36-37 0.22348567409861886 1108 38-39 0.11791618266900628 1108 40-41 0.2866408180988387 1108 42-43 0.22126337840726507 1108 44-45 0.15237737635267834 1108 46-47 0.21251718257582652 1108 48-49 0.25665154952614344 1108 50-51 0.23088718460488522 1108 52-53 0.10131996907159291 1108 54-55 0.24701564058953096 1108 56-57 0.16971016847194065 1108 58-59 0.16754226533116778 1108 60-61 0.1486525599415529 1108 62-63 0.12114856758313408 1108 64-65 0.012847336379682162 1108 66-67 -0.01876353295142863 1108 68-69 0.05095650265889162 1108 70-71 -0.05228029188920402 1108 72-73 0.1395877585926648 1108 74-75 0.04478166139814732 1108 76-77 0.11233723183394773 1109 1 -0.0031039173770111006 1109 2 -0.024168318517034493 1109 3 -0.10675477537255773 1109 4 0.11215430933113169 1109 5 0.15522430187649405 1109 6 0.11047413705216513 1109 7 -0.10047203061417065 1109 8 -0.012560185154626424 1109 9 -7.509233971489948E-4 1109 10-11 -0.11295168902567099 1109 12-13 0.11655584067135294 1109 14-15 0.10275199198953544 1109 16-17 0.13343304386479815 1109 18-19 0.013549281858644235 1109 20-21 0.08539518406549007 1109 22-23 0.03511488668669216 1109 24-25 -0.04055778651282793 1109 26-27 -0.0118024902160343 1109 28-29 0.19821857618175187 1109 30-31 0.27510486932384026 1109 32-33 0.15268848611943753 1109 34-35 0.21315603232310565 1109 36-37 0.23406438696358833 1109 38-39 0.2667209250590048 1109 40-41 0.28804753421504614 1109 42-43 0.33097055369781003 1109 44-45 0.2190234461352638 1109 46-47 0.15632854235317595 1109 48-49 0.21046652652487197 1109 50-51 0.20168046253089855 1109 52-53 0.31484641947097813 1109 54-55 0.3484524860320448 1109 56-57 0.32403376935198835 1109 58-59 0.2555378565766411 1109 60-61 0.1636712631038577 1109 62-63 0.1574440222253095 1109 64-65 0.1981523958084992 1109 66-67 0.18404266483823406 1109 68-69 0.2523227058956685 1109 70-71 0.2251263731603501 1109 72-73 0.24769646247841237 1109 74-75 0.18040312130869296 1109 76-77 0.21439743090878594 1110 1 -0.3271238855290193 1110 2 -0.3105454953847726 1110 3 -0.4421067725690335 1110 4 -0.2888091536718562 1110 5 -0.21423869514327265 1110 6 -0.53010526028946 1110 7 -0.471326611688454 1110 8 -0.3606386467170566 1110 9 -0.37396917163839305 1110 10-11 -0.251052556241838 1110 12-13 -0.32909383874346076 1110 14-15 -0.3556534601633814 1110 16-17 -0.37554325845967895 1110 18-19 -0.3097250799797351 1110 20-21 -0.4439133704316731 1110 22-23 -0.5258084662839124 1110 24-25 -0.4633889255309924 1110 26-27 -0.274087488937937 1110 28-29 -0.1463098922270376 1110 30-31 -0.20168314859139969 1110 32-33 -0.04665260597548837 1110 34-35 0.1182616402679244 1110 36-37 0.05665010151927419 1110 38-39 -0.019121921062421166 1110 40-41 0.05381132730192206 1110 42-43 -0.020619342605584734 1110 44-45 -0.10755722499330389 1110 46-47 -0.15290364460506467 1110 48-49 -0.05462226226023503 1110 50-51 -0.08598318278740535 1110 52-53 0.006911310328785447 1110 54-55 -0.012769255342789165 1110 56-57 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-0.8753074973862027 2314 34-35 -0.8912231466197724 2314 36-37 -1.0501946465408665 2314 38-39 -0.9959427672143306 2314 40-41 -0.7905037934383756 2314 42-43 -0.7378195837121737 2314 44-45 -0.7730239254079336 2314 46-47 -0.7158915657913916 2314 48-49 -0.6702928856146926 2314 50-51 -0.6236092483924018 2314 52-53 -0.6278432645642944 2314 54-55 -0.6547369355790948 2314 56-57 -0.5264066550535311 2314 58-59 -0.627498986859834 2314 60-61 -0.6233037652083269 2314 62-63 -0.558207697279375 2314 64-65 -0.5382479958148885 2314 66-67 -0.5681248217614261 2314 68-69 -0.4667253363291728 2314 70-71 -0.4933920356455559 2314 72-73 -0.3637568507116882 2314 74-75 -0.2605410434951807 2314 76-77 -0.28518808327920553 2315 1 -1.4171435694596823 2315 2 -1.2374974250020827 2315 3 -1.2254547641486013 2315 4 -1.2546946730094248 2315 5 -1.2476140656310477 2315 6 -1.2566467108733619 2315 7 -1.4037532398248729 2315 8 -1.3562367801855686 2315 9 -1.3218688543559622 2315 10-11 -1.3602380776493774 2315 12-13 -1.3976153933455535 2315 14-15 -1.633397350274194 2315 16-17 -1.4748336814018401 2315 18-19 -1.4495632748443867 2315 20-21 -1.5218835968618691 2315 22-23 -1.5372638315630738 2315 24-25 -1.5759841138713995 2315 26-27 -1.4789807978456757 2315 28-29 -1.4232761611714366 2315 30-31 -1.5976417329080874 2315 32-33 -1.6260379609192697 2315 34-35 -1.570848841268699 2315 36-37 -1.7423516755223005 2315 38-39 -1.598092801457394 2315 40-41 -1.6687965865143326 2315 42-43 -1.7303573944500457 2315 44-45 -1.6517037588355628 2315 46-47 -1.6458563136334199 2315 48-49 -1.5648378443851634 2315 50-51 -1.4603997111755405 2315 52-53 -1.5856254279538824 2315 54-55 -1.6080659981793843 2315 56-57 -1.6863060295248609 2315 58-59 -1.5254021368907686 2315 60-61 -1.619488393216173 2315 62-63 -1.7875946278988515 2315 64-65 -1.7167190899276186 2315 66-67 -1.6867387524569928 2315 68-69 -1.6765639031715232 2315 70-71 -1.8179376809315357 2315 72-73 -3.254247425322415 2315 74-75 -3.8172619338589264 2315 76-77 -3.5534398425785945 2316 1 -3.900255669667132 2316 2 -3.7861952397433285 2316 3 -3.2349491984779633 2316 4 -3.2776006837734215 2316 5 -3.9094468096476014 2316 6 -3.345267208677946 2316 7 -3.678988409947518 2316 8 -3.5806265801740764 2316 9 -3.2621289641331863 2316 10-11 -3.3471238534970524 2316 12-13 -3.347537598855247 2316 14-15 -3.439231302534857 2316 16-17 -4.8720083337895375 2316 18-19 -4.49858477410902 2316 20-21 -3.778758515878117 2316 22-23 -3.895907635989161 2316 24-25 -3.9400334748845935 2316 26-27 -3.5546982470546737 2316 28-29 -3.769377603583809 2316 30-31 -4.02846070734995 2316 32-33 -4.177627379666355 2316 34-35 -4.1735633997753645 2316 36-37 -4.001068816158174 2316 38-39 -4.0236823965319815 2316 40-41 -4.048647195712483 2316 42-43 -4.364032523723484 2316 44-45 -4.121807264413253 2316 46-47 -3.748526239241915 2316 48-49 -3.912622229249319 2316 50-51 -4.392534813438811 2316 52-53 -5.386594494500148 2316 54-55 -4.597226382065724 2316 56-57 -4.45998038402595 2316 58-59 -4.454490396818873 2316 60-61 -4.252081729906855 2316 62-63 -4.3225249551600164 2316 64-65 -4.219502526363559 2316 66-67 -4.288910435997227 2316 68-69 -4.2525700719623565 2316 70-71 -4.077583045588824 2316 72-73 -4.043251134878961 2316 74-75 -4.225585844134947 2316 76-77 -4.073579097888793 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 171184.0 3 13619.0 4 17379.0 5 20240.0 6 22941.0 7 24148.0 8 27937.0 9 29029.0 10 34176.0 11 37750.0 12 31401.0 13 27750.0 14 27636.0 15 28900.0 16 29195.0 17 31035.0 18 32530.0 19 34794.0 20 37447.0 21 40169.0 22 44632.0 23 50644.0 24 58076.0 25 67875.0 26 79310.0 27 93207.0 28 108507.0 29 127887.0 30 151497.0 31 180189.0 32 219152.0 33 272297.0 34 359134.0 35 473777.0 36 656180.0 37 859082.0 38 738629.0 39 181916.0 40 1161.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.750575854271887 26.56004366110092 28.408914876472714 22.280465608154483 2 22.540436381037157 26.81826350969668 28.15261171114188 22.48868839812428 3 22.674098188336885 26.89466589852224 27.86565190745146 22.565584005689416 4 22.379034184672946 26.861541232206587 28.26446903679419 22.494955546326278 5 22.605120093146063 26.955115388799538 28.14116200299314 22.29860251506126 6 22.818223280835912 26.71931109583055 28.135043563684714 22.32742205964882 7 22.607083748806872 26.502127506302397 28.272513397758225 22.618275347132506 8 22.6040216013411 26.965969397487537 27.92816484529857 22.501844155872796 9 22.50343240428549 26.71469980544043 28.131693326185818 22.65017446408826 10-11 22.917117876893283 26.803840088859737 27.912142580624472 22.36689945362251 12-13 22.949186975995428 26.506084873522195 27.88572410431369 22.659004046168683 14-15 22.635400246361428 26.75606019803916 28.048093308265365 22.56044624733405 16-17 22.86889907489841 26.607443259547548 28.11220106925537 22.411456596298677 18-19 22.821283183820015 26.602766871601403 27.81115865631251 22.76479128826607 20-21 22.646278258835167 26.874907017234168 27.772999927497565 22.705814796433103 22-23 22.79585671721337 26.854932266193742 27.45052112646936 22.89868989012352 24-25 22.85838938749009 26.985855359187205 27.350174068590384 22.80558118473232 26-27 22.804710895111974 26.985058739161804 27.284562118395773 22.925668247330446 28-29 23.027961077737302 27.012754835077814 27.230672023422375 22.728612063762508 30-31 22.79817772241395 26.76000706360423 27.3354225235259 23.10639269045592 32-33 22.71808553939301 27.138719119743282 27.250895694437222 22.89229964642649 34-35 22.884958225690518 26.82188058494967 27.352495308768614 22.940665880591197 36-37 22.89282303869291 26.888863713564344 27.510354271025967 22.707958976716775 38-39 22.794210611428824 26.878840725658254 27.554119316093562 22.772829346819364 40-41 22.658996810443853 26.836117234978058 27.509825292002333 22.99506066257576 42-43 22.590782341065434 27.059819444204525 27.290512679812455 23.058885534917586 44-45 22.5939589554745 26.787601981687974 27.566582983023117 23.051856079814403 46-47 22.54848248712586 26.786335216517127 27.594704418559346 23.070477877797668 48-49 22.689839798727725 26.75938418945941 27.422759723453698 23.128016288359166 50-51 22.463609682395482 26.977704683904996 27.357100483090456 23.201585150609066 52-53 22.505033950733868 26.830026857846967 27.434900532773 23.23003865864617 54-55 22.511615879291348 26.800152289987633 27.481891671018705 23.206340159702314 56-57 22.48720327236022 27.091727991259496 27.3299968344151 23.09107190196519 58-59 22.58227568025653 26.95651079279807 27.344248262242772 23.116965264702632 60-61 22.516442433907503 26.800632014308995 27.214473660633676 23.468451891149826 62-63 22.48955513825629 26.67306859079713 27.4327166197473 23.40465965119928 64-65 22.45070214155126 26.549133827081533 27.5019493083344 23.498214723032806 66-67 22.39596701336288 26.69556245939918 27.61434831781071 23.29412220942723 68-69 22.46571273270775 26.500517725741936 27.6153054177476 23.418464123802718 70-71 22.338010157652086 26.382475347673275 27.597902433446347 23.681612061228293 72-73 22.336867460219235 26.31190047484792 27.570253897712306 23.780978167220542 74-75 22.246365177944035 26.23310675060581 27.57948187751954 23.941046193930614 76-77 22.099240235168107 26.1218356618664 27.898543661466213 23.880380441499284 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12794.0 1 7802.5 2 2703.5 3 2709.0 4 2822.0 5 2949.5 6 3118.5 7 3344.5 8 3684.5 9 3840.0 10 3915.0 11 4111.5 12 4485.5 13 4738.0 14 4907.5 15 5379.0 16 6093.0 17 6505.0 18 7043.0 19 8260.5 20 10019.0 21 12728.5 22 14359.0 23 16818.0 24 22344.5 25 30065.0 26 34718.0 27 40474.5 28 54393.0 29 69410.0 30 76265.0 31 87714.5 32 110521.0 33 132717.5 34 152618.5 35 161680.0 36 173413.0 37 194102.0 38 205768.0 39 208478.0 40 213556.5 41 225509.5 42 241747.5 43 251111.0 44 252061.0 45 253357.0 46 258854.5 47 265555.0 48 267104.0 49 269240.0 50 268324.0 51 257929.0 52 250586.0 53 245203.5 54 226397.5 55 200666.5 56 174148.5 57 159938.0 58 152299.5 59 130941.5 60 106258.5 61 95295.0 62 84175.0 63 61965.0 64 43788.0 65 36701.0 66 32277.5 67 25218.0 68 19072.0 69 13333.0 70 11104.0 71 9352.0 72 6234.0 73 4363.5 74 3859.0 75 3275.0 76 2198.5 77 1384.5 78 1063.0 79 863.5 80 558.0 81 368.0 82 271.0 83 258.0 84 220.5 85 164.5 86 142.0 87 138.0 88 128.5 89 109.0 90 95.0 91 91.0 92 89.0 93 83.0 94 81.0 95 78.0 96 73.0 97 76.5 98 89.0 99 174.0 100 250.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009131980452784537 2 0.012016730817145046 3 0.01335804786554197 4 0.014552371264799505 5 0.01168599510658142 6 0.009076857834357266 7 0.015250591098211601 8 0.01650003711589641 9 0.016371417672899444 10-11 0.01887949681134027 12-13 0.015517017087276746 14-15 0.007487489003037624 16-17 0.00564088128572405 18-19 0.01696857937252821 20-21 0.022333847566115908 22-23 0.019743084500034175 24-25 0.018787625780628148 26-27 0.022747267204320437 28-29 0.008020340981167908 30-31 0.007919282847384579 32-33 0.010013942347620871 34-35 0.011217452849949617 36-37 0.010574355634964791 38-39 0.013449918896254087 40-41 0.01082240741788751 42-43 0.014148138729666186 44-45 0.012632266722916235 46-47 0.02337199021316284 48-49 0.022517589627540142 50-51 0.03526928869038213 52-53 0.038631768414445655 54-55 0.02712951536928847 56-57 0.019834955530746295 58-59 0.01549864288113432 60-61 0.019834955530746295 62-63 0.024612249127776435 64-65 0.025347217373473376 66-67 0.027221386400000585 68-69 0.04176457056172888 70-71 0.05449789541842844 72-73 0.0858259168912607 74-75 0.08567892324212131 76-77 0.09076857834357266 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 5442412.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.14323762142624 #Duplication Level Percentage of deduplicated Percentage of total 1 69.97905014611047 30.891018391214565 2 13.910809496761791 12.28136338243897 3 5.709580208213951 7.561180675493421 4 3.0830805509209216 5.443886294611999 5 1.8509761474690078 4.085403995465825 6 1.185212768931392 3.1391477334532194 7 0.8177245266295337 2.5267905661513055 8 0.593393273835644 2.0955440231906306 9 0.44588802629090213 1.7714646987397233 >10 2.1897358368966118 17.039564761538596 >50 0.14537683571281151 4.417053197808761 >100 0.0844731410345356 6.826904446556531 >500 0.003817973800456315 1.099513428879765 >1k 8.391118019327807E-4 0.5805268225420563 >5k 0.0 0.0 >10k+ 4.1955590095183574E-5 0.2406375819146316 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12993 0.23873606040851003 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.8374206142423617E-5 0.0 2 0.0 0.0 0.0 3.8585832899089593E-4 0.0 3 0.0 0.0 0.0 5.879745965575557E-4 1.8374206142423617E-5 4 0.0 0.0 0.0 0.0012494460176848059 1.8374206142423617E-5 5 0.0 0.0 0.0 0.0029766213950726257 3.6748412284847234E-5 6 0.0 0.0 0.0 0.005475513430442238 3.6748412284847234E-5 7 0.0 0.0 0.0 0.011318510983732948 3.6748412284847234E-5 8 0.0 0.0 0.0 0.01666540497117822 5.512261842727085E-5 9 0.0 0.0 0.0 0.02337199021316284 5.512261842727085E-5 10 0.0 0.0 0.0 0.031769002420250436 7.349682456969447E-5 11 0.0 0.0 0.0 0.041158221759028904 7.349682456969447E-5 12 0.0 0.0 0.0 0.05436927597543148 7.349682456969447E-5 13 0.0 0.0 0.0 0.07221063013972481 7.349682456969447E-5 14 0.0 0.0 0.0 0.09247737951481806 7.349682456969447E-5 15 0.0 0.0 0.0 0.11671295741667481 9.187103071211809E-5 16 0.0 0.0 0.0 0.14034218651583158 9.187103071211809E-5 17 0.0 0.0 0.0 0.16180325929018236 9.187103071211809E-5 18 0.0 0.0 0.0 0.1853957399770543 9.187103071211809E-5 19 0.0 0.0 0.0 0.2102009182693262 1.102452368545417E-4 20 0.0 0.0 0.0 0.2381664600180949 1.102452368545417E-4 21 0.0 0.0 0.0 0.26719770572312423 1.102452368545417E-4 22 0.0 0.0 0.0 0.29955468273993224 1.102452368545417E-4 23 0.0 0.0 0.0 0.3336939577525553 1.102452368545417E-4 24 0.0 0.0 0.0 0.3679251037958905 1.4699364913938894E-4 25 0.0 0.0 0.0 0.406768175580974 1.4699364913938894E-4 26 0.0 0.0 0.0 0.4458868604581939 1.6536785528181255E-4 27 0.0 0.0 0.0 0.48436244812042895 1.8374206142423617E-4 28 0.0 0.0 0.0 0.5252266825811791 1.8374206142423617E-4 29 0.0 0.0 0.0 0.5652640777655201 2.3886467985150702E-4 30 0.0 0.0 0.0 0.609325424095052 2.3886467985150702E-4 31 0.0 0.0 0.0 0.6584764255260351 2.3886467985150702E-4 32 0.0 0.0 0.0 0.7109164098565122 2.3886467985150702E-4 33 0.0 0.0 0.0 0.7688319076174314 2.3886467985150702E-4 34 0.0 0.0 0.0 0.8284562065495961 2.3886467985150702E-4 35 0.0 0.0 0.0 0.8902302876004242 2.3886467985150702E-4 36 0.0 0.0 0.0 0.9544848864804796 2.3886467985150702E-4 37 0.0 0.0 0.0 1.0222857071460227 2.3886467985150702E-4 38 0.0 0.0 0.0 1.0890391980614478 2.3886467985150702E-4 39 0.0 0.0 0.0 1.1587693103719454 2.3886467985150702E-4 40 0.0 0.0 0.0 1.2324315027969217 2.3886467985150702E-4 41 0.0 0.0 0.0 1.3134066292665825 2.3886467985150702E-4 42 0.0 0.0 0.0 1.3984792037060039 2.3886467985150702E-4 43 0.0 0.0 0.0 1.4898173824399916 2.5723888599393064E-4 44 0.0 0.0 0.0 1.588248004744955 2.5723888599393064E-4 45 0.0 0.0 0.0 1.692870734519915 2.939872982787779E-4 46 0.0 0.0 0.0 1.8051003856378385 3.1236150442120146E-4 47 0.0 0.0 0.0 1.919847302997274 3.1236150442120146E-4 48 0.0 0.0 0.0 2.0382874357913368 3.8585832899089593E-4 49 0.0 0.0 0.0 2.1564887039055476 3.8585832899089593E-4 50 0.0 0.0 0.0 2.2761047858927257 3.8585832899089593E-4 51 0.0 0.0 0.0 2.404889596744973 3.8585832899089593E-4 52 0.0 0.0 0.0 2.544313072953683 3.8585832899089593E-4 53 0.0 0.0 0.0 2.6921151871633384 3.8585832899089593E-4 54 0.0 0.0 0.0 2.8515665480672907 3.8585832899089593E-4 55 0.0 0.0 0.0 3.017871487862367 3.8585832899089593E-4 56 0.0 0.0 0.0 3.198692050509958 3.8585832899089593E-4 57 0.0 0.0 0.0 3.379622858394403 4.2260674127574317E-4 58 0.0 0.0 0.0 3.5664517864505663 4.409809474181668E-4 59 0.0 0.0 0.0 3.7568636847045025 4.409809474181668E-4 60 0.0 0.0 0.0 3.9497377265815232 4.409809474181668E-4 61 0.0 0.0 0.0 4.151743748911328 4.409809474181668E-4 62 0.0 0.0 0.0 4.357259244614337 4.409809474181668E-4 63 0.0 0.0 0.0 4.583096612310865 4.409809474181668E-4 64 0.0 0.0 0.0 4.821832672719375 4.409809474181668E-4 65 0.0 0.0 0.0 5.073357180603012 4.409809474181668E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCGCG 750 0.0 21.772398 56 GACGTAT 970 0.0 21.59007 39 CGCGGAA 810 0.0 20.59807 59 TCGCGCT 830 0.0 20.100622 37 CGGTTAC 680 0.0 19.835377 4 CTCGCGC 835 0.0 19.55371 36 CGATCGT 900 0.0 18.930431 1 TGCGTAA 1005 0.0 18.719728 37 AATTCGC 900 0.0 18.143164 55 CAATTCG 910 0.0 17.945604 54 TTGCGTA 1040 0.0 17.747116 36 CGGAGCG 690 0.0 17.490555 35 CGCGCTT 935 0.0 17.462885 38 ACGTATT 1205 0.0 17.380198 40 GCGTAAG 1065 0.0 17.330996 38 CGTAAGT 1075 0.0 16.509552 39 TCGCGGA 1035 0.0 16.119932 58 CCTCGCG 1050 0.0 15.888478 35 ATCGTTC 1130 0.0 15.705413 3 CGCTCCG 1090 0.0 15.305416 25 >>END_MODULE