FastQCFastQC Report
Thu 26 May 2016
SRR936087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5442412
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126910.23318705015349814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93710.1721846857606517No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64860.11917510103975958No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA23850.047.168804100-101
CGTACAT34000.040.615383100-101
GGTATCA154250.035.9552571
CGGTATC22500.033.464893100-101
TCGTATC9900.031.564981100-101
CCGTACA16800.031.532774100-101
GTATCAA297250.031.0099621
CGAGTAC25250.030.055935100-101
TACGCAG22250.029.961906100-101
ACGTATC9950.027.817066100-101
GCGTATC8000.027.529173100-101
AGTATCA63550.027.302628100-101
AGGTATC42500.027.170288100-101
CCTGTCG18200.027.1400396-97
GCGTACA15150.027.109276100-101
TCGAGTA10850.026.881147100-101
CCGTATC13250.026.504375100-101
TCCGGTA5900.026.22979298-99
AGCGTAC6800.025.82180498-99
TCGTACA18350.025.463322100-101
Produced by FastQC (version 0.11.5)