FastQCFastQC Report
Thu 26 May 2016
SRR936086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5478197
Sequences flagged as poor quality0
Sequence length77
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130490.23819880884166816No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG6950.030.13479656
CGCGGAA7550.025.39284159
CGATCGT8800.024.6034871
TCGCGCT8300.024.37642937
AATTCGC8900.023.93129755
CAATTCG9050.023.14261254
CGCGCTT9100.023.01398738
CTCGCGC9300.022.13556336
CGCTCCG9800.021.0077325
CGGTTAC7100.020.9961874
TCGCGGA9650.020.97027458
CCTCGCG10350.019.889235
CGGCCAA10450.019.36701850
GACGTAT8100.018.4062539
AACTCGA11850.018.2722813
ATCGTTC12450.017.6755053
ACTCGAG12300.017.6036214
GATCGTT12900.017.0589182
TTCGCGG13300.015.74957
ACGATCG6650.015.482491559
Produced by FastQC (version 0.11.5)