##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936086_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5478197 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.07250305164272 39.0 36.0 41.0 28.0 41.0 2 35.936167136742256 39.0 35.0 41.0 27.0 41.0 3 35.79628644241892 39.0 35.0 41.0 27.0 41.0 4 35.68093571662355 39.0 35.0 40.0 27.0 41.0 5 35.60667204921619 39.0 35.0 40.0 27.0 41.0 6 35.516337218249 39.0 35.0 40.0 27.0 41.0 7 35.36382444808027 38.0 34.0 40.0 27.0 41.0 8 35.235778486973 38.0 34.0 40.0 26.0 41.0 9 35.401672849662035 39.0 35.0 40.0 27.0 41.0 10-11 35.62344234791118 39.0 35.0 40.5 27.0 41.0 12-13 35.53344905267189 39.0 35.0 41.0 26.5 41.0 14-15 35.37850007949696 39.0 35.0 41.0 25.5 41.0 16-17 35.222037834710946 39.0 35.0 41.0 25.0 41.0 18-19 35.05894704042224 39.0 34.5 41.0 25.0 41.0 20-21 34.84676135232085 39.0 34.0 41.0 24.0 41.0 22-23 34.5642737017307 38.5 34.0 40.5 23.5 41.0 24-25 34.329067483334384 38.0 33.0 40.0 22.5 41.0 26-27 33.38416855034603 37.5 32.0 39.5 20.5 40.5 28-29 33.39724684964779 37.5 32.5 39.5 19.5 40.5 30-31 33.79581274642004 38.0 33.0 40.0 20.0 41.0 32-33 33.67070287906769 38.0 33.0 40.0 18.5 41.0 34-35 33.41041486824953 38.0 33.0 40.0 17.5 41.0 36-37 33.095238360358344 37.0 32.0 40.0 14.5 41.0 38-39 32.780427940068606 37.0 32.0 40.0 12.5 41.0 40-41 32.413072494472175 36.0 31.5 39.5 8.0 41.0 42-43 32.03303687326323 36.0 31.0 39.0 7.5 41.0 44-45 31.667875123877437 35.0 31.0 39.0 6.0 40.5 46-47 31.241192585808797 35.0 31.0 38.0 2.0 40.0 48-49 30.817566710361092 35.0 30.0 37.5 2.0 39.5 50-51 30.356074270421452 35.0 30.0 37.0 2.0 39.0 52-53 29.867051239668818 34.5 29.0 36.0 2.0 39.0 54-55 29.402077362314643 34.0 29.0 36.0 2.0 38.5 56-57 28.95773326516005 34.0 29.0 35.5 2.0 37.0 58-59 28.530979444514315 34.0 28.5 35.0 2.0 37.0 60-61 28.16586022737043 34.0 27.5 35.0 2.0 36.0 62-63 27.785089327747798 34.0 27.0 35.0 2.0 36.0 64-65 27.400989504393507 33.5 26.0 35.0 2.0 35.5 66-67 27.03170523075384 33.0 26.0 35.0 2.0 35.0 68-69 26.645622820793044 33.0 25.0 35.0 2.0 35.0 70-71 26.17792879664605 33.0 24.5 35.0 2.0 35.0 72-73 25.67541747768472 33.0 22.0 35.0 2.0 35.0 74-75 25.07290172295739 32.0 19.0 35.0 2.0 35.0 76-77 23.317208380786596 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.1061347690529786 1101 2 1.0370185524977131 1101 3 1.0801056092248018 1101 4 1.0614756273769856 1101 5 1.0631528313998615 1101 6 1.1981553672291057 1101 7 1.1868708761322253 1101 8 1.1639502606934826 1101 9 0.9743461757473142 1101 10-11 1.1073466228533206 1101 12-13 1.2825288746666104 1101 14-15 1.2558451809858227 1101 16-17 1.271197196652487 1101 18-19 1.201560080569088 1101 20-21 1.1473261051558694 1101 22-23 1.2025786163295251 1101 24-25 1.2859215000452409 1101 26-27 1.2816033039222319 1101 28-29 1.2892389423515098 1101 30-31 1.3170523745018201 1101 32-33 1.3596004790598855 1101 34-35 1.360485217546369 1101 36-37 1.3383753107343423 1101 38-39 1.330531047768993 1101 40-41 1.2585389866421863 1101 42-43 1.2793602567691025 1101 44-45 1.3146950223782774 1101 46-47 1.2490029121879331 1101 48-49 1.291232745676563 1101 50-51 1.2357304797600932 1101 52-53 1.3257928686442462 1101 54-55 1.373355912024035 1101 56-57 1.4214387204039696 1101 58-59 1.4063319020765412 1101 60-61 1.37431746868171 1101 62-63 1.4389833978486521 1101 64-65 1.2889607181893723 1101 66-67 1.3841709837021803 1101 68-69 1.3530599870777067 1101 70-71 1.3523474455716205 1101 72-73 1.3676079185357821 1101 74-75 1.3973491235911943 1101 76-77 1.4665972663052997 1102 1 1.2050685674886878 1102 2 1.0995810479764287 1102 3 1.1396796415276285 1102 4 1.0359800013308984 1102 5 1.0264814829337183 1102 6 1.0361466400152963 1102 7 1.0671368702611446 1102 8 1.1586819185899557 1102 9 1.2636268712925656 1102 10-11 1.2546681365443604 1102 12-13 1.3558383373668903 1102 14-15 1.3119826451272516 1102 16-17 1.3344843186617297 1102 18-19 1.3513299083082373 1102 20-21 1.3760231658454032 1102 22-23 1.3143068689111104 1102 24-25 1.358941794858879 1102 26-27 1.2726430967415467 1102 28-29 1.39871287399653 1102 30-31 1.5374223587018534 1102 32-33 1.491778821129813 1102 34-35 1.4782080129220176 1102 36-37 1.5014070473918792 1102 38-39 1.5790761259631765 1102 40-41 1.6135886708462337 1102 42-43 1.5750015492145977 1102 44-45 1.4494733815340233 1102 46-47 1.534499645452179 1102 48-49 1.4418361176687888 1102 50-51 1.3970003422545645 1102 52-53 1.348863969914202 1102 54-55 1.4497047416360722 1102 56-57 1.4948929350758853 1102 58-59 1.5336753620342414 1102 60-61 1.4370905832315657 1102 62-63 1.369892047221139 1102 64-65 1.381016003396212 1102 66-67 1.4405078532356228 1102 68-69 1.3643773838981517 1102 70-71 1.3282475688336532 1102 72-73 1.2964819926334386 1102 74-75 1.2646299956546123 1102 76-77 1.250688310060049 1103 1 0.627253593823653 1103 2 0.5908983365139946 1103 3 0.5646981355721294 1103 4 0.5693508768819058 1103 5 0.4723009284171553 1103 6 0.5737851721838467 1103 7 0.5527471183168444 1103 8 0.4784147870093278 1103 9 0.575522109110743 1103 10-11 0.6600830299992637 1103 12-13 0.7131550350647728 1103 14-15 0.6629886137857355 1103 16-17 0.6945302212957856 1103 18-19 0.6260387656394926 1103 20-21 0.689287690250616 1103 22-23 0.585503225792138 1103 24-25 0.7704640867627788 1103 26-27 0.7011028164355579 1103 28-29 0.6365659232280905 1103 30-31 0.7787992680214657 1103 32-33 0.8036857649162314 1103 34-35 0.7967573490758681 1103 36-37 0.8263654357917503 1103 38-39 0.7811166009068629 1103 40-41 0.7816028572329614 1103 42-43 0.7189100300154223 1103 44-45 0.6963420696620979 1103 46-47 0.7390328682368406 1103 48-49 0.6692144033054959 1103 50-51 0.612584622455266 1103 52-53 0.8090103776365396 1103 54-55 0.8453132026268015 1103 56-57 0.7543655396468942 1103 58-59 0.7799981468356023 1103 60-61 0.6541300399638139 1103 62-63 0.5982917478531213 1103 64-65 0.5618870659656494 1103 66-67 0.6848351860480726 1103 68-69 0.6759270185888226 1103 70-71 0.6497673636952186 1103 72-73 0.6481566733860546 1103 74-75 0.6699573367789959 1103 76-77 0.7840125695363405 1104 1 0.6063460855985525 1104 2 0.5495496271298634 1104 3 0.5822014114405931 1104 4 0.6425008560602521 1104 5 0.5740438975640316 1104 6 0.6061978738323575 1104 7 0.6832455081811659 1104 8 0.6718924293433588 1104 9 0.6016572438628529 1104 10-11 0.6092079343395511 1104 12-13 0.6283922793453485 1104 14-15 0.7560050863349446 1104 16-17 0.7734387248180283 1104 18-19 0.6914786668074697 1104 20-21 0.6712745017459056 1104 22-23 0.744747906457647 1104 24-25 0.8086092175467883 1104 26-27 0.5996417164196401 1104 28-29 0.5960951847459555 1104 30-31 0.8417836305189255 1104 32-33 0.8363952569767434 1104 34-35 0.9145426030892096 1104 36-37 0.7917819262192296 1104 38-39 0.8104853612223053 1104 40-41 0.9130802602814256 1104 42-43 0.9395807191142467 1104 44-45 0.8505488080308545 1104 46-47 0.7518157048663952 1104 48-49 0.6960068920280058 1104 50-51 0.7802628929275386 1104 52-53 0.800741262589785 1104 54-55 0.6565416914539632 1104 56-57 0.7514128293426765 1104 58-59 0.6965342920377893 1104 60-61 0.7455488453020074 1104 62-63 0.6276703875555576 1104 64-65 0.4989184423536699 1104 66-67 0.564295286406292 1104 68-69 0.4151122921156656 1104 70-71 0.4660734486345106 1104 72-73 0.3224662609427682 1104 74-75 0.2887836737802836 1104 76-77 0.3565346285041535 1105 1 0.1670088044200071 1105 2 0.4028472720972829 1105 3 0.30062812159943064 1105 4 0.3078210814459581 1105 5 0.22702939025881363 1105 6 0.211036942758966 1105 7 0.2678614322397692 1105 8 0.3145377767639559 1105 9 0.2847402416207956 1105 10-11 0.5096757847748208 1105 12-13 0.5617330585354168 1105 14-15 0.4572072485334431 1105 16-17 0.43254401654419183 1105 18-19 0.31461900249940555 1105 20-21 0.2367754525522301 1105 22-23 0.22110551708615844 1105 24-25 0.2727173926722557 1105 26-27 0.1963682043974373 1105 28-29 0.40382889431609925 1105 30-31 0.5770660268127372 1105 32-33 0.5549350555140649 1105 34-35 0.5156495361661371 1105 36-37 0.5715713321026357 1105 38-39 0.450591720994133 1105 40-41 0.5731626308730142 1105 42-43 0.6477331931612937 1105 44-45 0.46225550706106944 1105 46-47 0.448856275482477 1105 48-49 0.32948296892567086 1105 50-51 0.32971023802008403 1105 52-53 0.401635096124501 1105 54-55 0.22067083399499765 1105 56-57 0.23243439908631558 1105 58-59 0.3760698885875904 1105 60-61 0.4394484545447064 1105 62-63 0.3808222494202127 1105 64-65 0.423205553599292 1105 66-67 0.33401522005031126 1105 68-69 0.3319879298618993 1105 70-71 0.26336064033453255 1105 72-73 -0.17119665577491006 1105 74-75 -0.3054913004601367 1105 76-77 -0.42801765085944155 1106 1 0.005505125916826614 1106 2 -0.01516328569714176 1106 3 -0.07653764960402043 1106 4 0.1090990326372605 1106 5 0.048438662921633124 1106 6 0.007171589096422792 1106 7 -0.33926205092685535 1106 8 -0.1441268144429131 1106 9 0.0366223922963087 1106 10-11 -0.26135448992165067 1106 12-13 0.10751691229103955 1106 14-15 0.09313171041060997 1106 16-17 0.0027516477786306837 1106 18-19 0.06453277126151136 1106 20-21 0.08137962900747908 1106 22-23 0.12707488238383036 1106 24-25 -0.15082359770968168 1106 26-27 -0.17529272761593262 1106 28-29 0.11380831871906594 1106 30-31 0.22031439516147344 1106 32-33 0.11120101041594666 1106 34-35 0.08026201255847809 1106 36-37 0.02940085168009432 1106 38-39 0.2038042867782579 1106 40-41 0.11459477489628256 1106 42-43 0.19158424441136646 1106 44-45 0.06180499193747835 1106 46-47 0.07594473152876446 1106 48-49 0.057692976219197334 1106 50-51 -0.051227867498557345 1106 52-53 -0.04945660068785784 1106 54-55 -0.09638448717802461 1106 56-57 -0.09485928306039781 1106 58-59 -0.235259233434558 1106 60-61 -0.36300263209117034 1106 62-63 -0.27144304477461745 1106 64-65 -0.15308449204323082 1106 66-67 -0.23687018616649524 1106 68-69 -0.1257652510815177 1106 70-71 -0.21682607903924733 1106 72-73 -0.21538804656360355 1106 74-75 -0.11246497988413395 1106 76-77 -0.3582244730232347 1107 1 -0.10546474737899558 1107 2 -0.1529698181967305 1107 3 0.016204584306443337 1107 4 -0.008440020798104797 1107 5 -0.03942541145614342 1107 6 -0.2783797234274772 1107 7 -0.17850009797644617 1107 8 -0.2270631928517517 1107 9 -0.3574302277831052 1107 10-11 -0.08086037576433824 1107 12-13 0.0023780377765660887 1107 14-15 -0.3116061843439226 1107 16-17 -0.17809600320651242 1107 18-19 -0.04728025345982445 1107 20-21 -0.08981239751338421 1107 22-23 -0.10951054747597055 1107 24-25 -0.162951768520756 1107 26-27 -0.480807076522872 1107 28-29 -0.3391204198739146 1107 30-31 -0.002939836149515429 1107 32-33 0.08184757590444747 1107 34-35 -0.09762863752763451 1107 36-37 -0.11365020357868616 1107 38-39 8.655966690511718E-4 1107 40-41 -0.06394265002873567 1107 42-43 -0.04009486681061247 1107 44-45 -0.11974943784359127 1107 46-47 -0.1377595825345992 1107 48-49 -0.05555203482772342 1107 50-51 -0.07411207932225494 1107 52-53 -0.085465019523145 1107 54-55 -0.06531364211295809 1107 56-57 -0.13515112933350437 1107 58-59 -0.13843614072741417 1107 60-61 -0.20212420954360155 1107 62-63 -0.14899295494559794 1107 64-65 -0.27638805338864714 1107 66-67 -0.23023495744526912 1107 68-69 -0.373699537460503 1107 70-71 -0.293930574087522 1107 72-73 -0.33933705587910623 1107 74-75 -0.4617748459205089 1107 76-77 -0.4948222280201513 1108 1 0.25273434132171246 1108 2 0.43989198820496256 1108 3 0.4836393791610831 1108 4 0.31046750484803454 1108 5 0.41515582035093956 1108 6 0.3802957675937151 1108 7 0.45575212141617527 1108 8 0.34055410947880205 1108 9 0.3600984862620251 1108 10-11 0.44368208100032547 1108 12-13 0.5652823297540266 1108 14-15 0.5390939530835794 1108 16-17 0.5975155125786245 1108 18-19 0.5311722065185904 1108 20-21 0.4442061619178119 1108 22-23 0.438415437220236 1108 24-25 0.4985771995956867 1108 26-27 0.4676565693575796 1108 28-29 0.5078351290971383 1108 30-31 0.6291350281272869 1108 32-33 0.6545117062642518 1108 34-35 0.656131675236999 1108 36-37 0.6229625300296888 1108 38-39 0.5673737107999486 1108 40-41 0.5325571267279727 1108 42-43 0.5603064001574793 1108 44-45 0.5435582156077352 1108 46-47 0.552407981961359 1108 48-49 0.5860004991162242 1108 50-51 0.5164059285712668 1108 52-53 0.6815692623854765 1108 54-55 0.7323315003883692 1108 56-57 0.7026459061061239 1108 58-59 0.695541245374681 1108 60-61 0.7175120706848865 1108 62-63 0.7107047138508555 1108 64-65 0.7126468054864965 1108 66-67 0.7598206083966375 1108 68-69 0.6716446508119205 1108 70-71 0.632799548967327 1108 72-73 0.6662654678048625 1108 74-75 0.7102560672470837 1108 76-77 0.6593574451395163 1109 1 -0.10877943200013362 1109 2 -0.039463854589882885 1109 3 -0.14240061443717167 1109 4 0.02271544314885432 1109 5 0.024670004729635764 1109 6 -0.15969055309675184 1109 7 0.006266378507767456 1109 8 0.12159318365731053 1109 9 0.0972028479629472 1109 10-11 -0.11607303464046481 1109 12-13 -0.04376695321464297 1109 14-15 0.1210561610737031 1109 16-17 0.015485626386542606 1109 18-19 -0.12657810668326164 1109 20-21 -0.0630485335378026 1109 22-23 0.003634058045449251 1109 24-25 -0.18304789723197956 1109 26-27 -0.26126007876489155 1109 28-29 -0.042609975458553606 1109 30-31 0.07214775489161696 1109 32-33 0.04954949658021235 1109 34-35 0.11897558950956721 1109 36-37 0.17052868386445397 1109 38-39 0.180576647346939 1109 40-41 0.1966145935047976 1109 42-43 0.2051843663997559 1109 44-45 0.06898312021989739 1109 46-47 0.1426483868548658 1109 48-49 0.1919009184607141 1109 50-51 0.09223313500142183 1109 52-53 0.1092706860462549 1109 54-55 0.22532002298090603 1109 56-57 0.13341645388266699 1109 58-59 0.2659017626864646 1109 60-61 0.191251265777602 1109 62-63 0.14093254989061776 1109 64-65 0.1784284713094486 1109 66-67 0.14028728967280202 1109 68-69 0.10716437329082851 1109 70-71 0.097743557841941 1109 72-73 -0.018092448154462204 1109 74-75 -0.02828216263997163 1109 76-77 -0.021485470475695934 1110 1 -0.35462556303708936 1110 2 -0.3947043443414202 1110 3 -0.6205888796739529 1110 4 -0.5089341942323884 1110 5 -0.6311652539219423 1110 6 -0.5492336040363455 1110 7 -0.4914015566038543 1110 8 -0.798457718874225 1110 9 -0.6803064491620532 1110 10-11 -0.5553192262168452 1110 12-13 -0.462279197003177 1110 14-15 -0.41538100148081014 1110 16-17 -0.5546813821013288 1110 18-19 -0.6001616078384586 1110 20-21 -0.7269524611870253 1110 22-23 -0.6003301928101337 1110 24-25 -0.6431362662506217 1110 26-27 -0.7169426915967776 1110 28-29 -0.5022791090035668 1110 30-31 -0.3745235345979978 1110 32-33 -0.30262473339544727 1110 34-35 -0.39006310012090495 1110 36-37 -0.27324740059421515 1110 38-39 -0.4258446699413909 1110 40-41 -0.36698700770104864 1110 42-43 -0.34657689825634463 1110 44-45 -0.3812553927343032 1110 46-47 -0.4792342964004206 1110 48-49 -0.4502279378384735 1110 50-51 -0.5490502388974363 1110 52-53 -0.5078429427359161 1110 54-55 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-1.2622582975949612 2314 42-43 -1.0865073020942688 2314 44-45 -1.1016560774344093 2314 46-47 -0.9509017326039384 2314 48-49 -0.9518531433611201 2314 50-51 -0.8782105803465683 2314 52-53 -0.7982614206295615 2314 54-55 -0.89503367408728 2314 56-57 -0.8464439306087748 2314 58-59 -0.8876968271636656 2314 60-61 -0.8382998995454791 2314 62-63 -0.9431652113888163 2314 64-65 -0.8133656247176937 2314 66-67 -0.7924498475891042 2314 68-69 -0.7391635818023019 2314 70-71 -0.6156942534863887 2314 72-73 -0.5727927409917797 2314 74-75 -0.5268914311177078 2314 76-77 -0.5934525576957199 2315 1 -1.4321291924955162 2315 2 -1.453068474839327 2315 3 -1.2833951188581665 2315 4 -1.2209153558913641 2315 5 -1.2293946589230416 2315 6 -1.2974214880330521 2315 7 -1.6582584456063074 2315 8 -1.6451842377299926 2315 9 -1.3608307752442101 2315 10-11 -1.5047657773602623 2315 12-13 -1.4798743759209572 2315 14-15 -1.4799875391516863 2315 16-17 -1.410712641900865 2315 18-19 -1.310610673470677 2315 20-21 -1.3496429061295956 2315 22-23 -1.5505283612402323 2315 24-25 -1.6035756630055928 2315 26-27 -1.4623730001878528 2315 28-29 -1.472079808182439 2315 30-31 -1.574886799833017 2315 32-33 -1.587061964089635 2315 34-35 -1.664620102943168 2315 36-37 -1.7925908751183215 2315 38-39 -1.665999081620697 2315 40-41 -1.7891261233907585 2315 42-43 -1.797892272061528 2315 44-45 -1.7375556099210954 2315 46-47 -1.5899596364080892 2315 48-49 -1.54727056800537 2315 50-51 -1.5772814441099214 2315 52-53 -1.6228150104238601 2315 54-55 -1.7433626346351936 2315 56-57 -1.7990249666531248 2315 58-59 -1.6458962398185797 2315 60-61 -1.750478961258512 2315 62-63 -1.6327470189588311 2315 64-65 -1.4887044569221928 2315 66-67 -1.5271135750370455 2315 68-69 -1.794002485395474 2315 70-71 -1.796689620592467 2315 72-73 -1.6373545014342668 2315 74-75 -1.6204432904241628 2315 76-77 -1.707776596055087 2316 1 -2.521418155102232 2316 2 -2.271476740009554 2316 3 -2.305465144376221 2316 4 -3.333743849663726 2316 5 -2.6083468855577863 2316 6 -2.559698671390038 2316 7 -2.240812397926206 2316 8 -2.008513194233977 2316 9 -2.405583502300587 2316 10-11 -2.300151618777832 2316 12-13 -2.966437502447789 2316 14-15 -4.266470198997688 2316 16-17 -3.36056126856133 2316 18-19 -2.8755228126312176 2316 20-21 -2.6630532619223786 2316 22-23 -2.988067273912911 2316 24-25 -2.866541284925816 2316 26-27 -2.840863996853667 2316 28-29 -3.439972771089362 2316 30-31 -3.7437947974554007 2316 32-33 -3.73936625187968 2316 34-35 -3.6371695616733035 2316 36-37 -3.6700038559970736 2316 38-39 -3.772098982713672 2316 40-41 -3.500864521731714 2316 42-43 -3.733978845396159 2316 44-45 -3.5662357141936205 2316 46-47 -3.5696516076903393 2316 48-49 -3.3129656015296227 2316 50-51 -3.3416439547108325 2316 52-53 -3.423996694281392 2316 54-55 -3.4766616174349316 2316 56-57 -3.429092655656248 2316 58-59 -3.6270374206697262 2316 60-61 -3.3458192692771114 2316 62-63 -3.2455315378940277 2316 64-65 -3.284226791049644 2316 66-67 -3.365493513583555 2316 68-69 -3.4705026390703004 2316 70-71 -3.3747939105701974 2316 72-73 -3.3279652983285004 2316 74-75 -3.509832080021038 2316 76-77 -3.7036704812006533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 172733.0 3 13948.0 4 17729.0 5 21571.0 6 23884.0 7 24970.0 8 28486.0 9 29943.0 10 34492.0 11 38049.0 12 31608.0 13 27878.0 14 28434.0 15 29608.0 16 29819.0 17 31586.0 18 33441.0 19 35312.0 20 37853.0 21 40963.0 22 45032.0 23 51281.0 24 58806.0 25 68291.0 26 79704.0 27 93509.0 28 109535.0 29 128025.0 30 151476.0 31 180371.0 32 219504.0 33 272381.0 34 357355.0 35 472676.0 36 658906.0 37 868675.0 38 749346.0 39 179893.0 40 1124.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.727942345581496 26.556461377735275 28.427484461079818 22.288111815603415 2 22.58531447965164 26.802054050166095 28.163576993017042 22.449054477165223 3 22.73396364921605 26.9097926800036 27.841117896965645 22.515125773814706 4 22.42071870991889 26.840697987557455 28.268642137598306 22.46994116492535 5 22.57136488171775 26.938306499978363 28.150239281096002 22.340089337207885 6 22.789257103631595 26.724168778343326 28.13914427802566 22.347429839999418 7 22.59962685742861 26.475321707210338 28.29453323676442 22.630518198596636 8 22.536414724365457 26.92712308158578 27.989731942349895 22.546730251698868 9 22.540360611884147 26.684779175519964 28.114201918263305 22.66065829433259 10-11 22.88044628314346 26.802540632649517 27.944996413222345 22.372016670984678 12-13 22.961844568390884 26.531627977375607 27.881439748100135 22.625087706133375 14-15 22.648378385878 26.76348801479147 28.051735456442717 22.536398142887812 16-17 22.848746972810886 26.611342690038835 28.108735647207826 22.43117468994245 18-19 22.79856738190467 26.601398734172594 27.829736574408436 22.770297309514298 20-21 22.642262244512253 26.8623964222199 27.779278337275002 22.716062995992854 22-23 22.807759514495416 26.84030954367922 27.459294662951244 22.89263627887412 24-25 22.874139793226288 26.977159838950616 27.337177502054743 22.811522865768353 26-27 22.821310753972128 27.001266373835787 27.25889535651669 22.918527515675404 28-29 23.053501141248876 26.97443905559396 27.242047905982197 22.730011897174965 30-31 22.773041613569468 26.755292780262618 27.335045283494548 23.136620322673366 32-33 22.733607919457413 27.137117897233665 27.247054579583015 22.882219603725908 34-35 22.877360234372397 26.828428287349904 27.34568878839895 22.948522689878747 36-37 22.917307332632493 26.88367203853954 27.49743345677021 22.701587172057756 38-39 22.782288910221325 26.861596760748057 27.54953431554225 22.80658001348836 40-41 22.684373536636464 26.829595794458637 27.507984887362657 22.978045781542246 42-43 22.593935970473304 27.08621946241137 27.282975264671883 23.036869302443446 44-45 22.629041576789948 26.812052957558347 27.53414919743806 23.024756268213643 46-47 22.551363513625276 26.77660621151034 27.58338395149566 23.08864632336872 48-49 22.696280673940088 26.733456499356766 27.44622683939121 23.124035987311935 50-51 22.45858556791536 27.000168349123214 27.335332829138054 23.20591325382337 52-53 22.504336990730557 26.824108491737007 27.422736924410046 23.248817593122386 54-55 22.555534811705435 26.784337551232618 27.46500159991382 23.19512603714813 56-57 22.50957732868501 27.081856618350148 27.324335310522525 23.084230742442315 58-59 22.55578979501035 26.958277443580332 27.366535087262868 23.119397674146448 60-61 22.523314183429374 26.79327232580227 27.23653302144295 23.446880469325404 62-63 22.466168949975536 26.636479388870583 27.500020312917528 23.39733134823635 64-65 22.46424096936359 26.53503382643081 27.502986081264496 23.4977391229411 66-67 22.389830028819972 26.675314787647853 27.605258617463058 23.329596566069117 68-69 22.487627523898848 26.50935900316466 27.596142951844964 23.406870521091527 70-71 22.332707407892432 26.37599945790875 27.606261665326965 23.685031468871852 72-73 22.369940063670022 26.29911063634218 27.567588203875587 23.763361096112213 74-75 22.235383817041736 26.233090362457716 27.58164129248607 23.949884528014483 76-77 22.10770590626818 26.088586761081658 27.903579281619013 23.900128051031153 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12747.0 1 7794.5 2 2636.5 3 2607.5 4 2784.0 5 2848.0 6 3092.0 7 3374.5 8 3605.0 9 3733.0 10 3874.5 11 4172.5 12 4516.0 13 4703.0 14 4864.5 15 5354.0 16 6091.5 17 6501.0 18 7032.0 19 8308.5 20 10144.5 21 12853.0 22 14471.0 23 16879.5 24 22364.0 25 30238.5 26 35037.0 27 40745.0 28 54959.0 29 70194.5 30 76924.0 31 88336.0 32 111024.5 33 133587.0 34 153823.0 35 162773.0 36 174747.0 37 195109.5 38 207095.5 39 210693.0 40 214806.5 41 226965.5 42 243259.0 43 251507.0 44 252952.0 45 254988.5 46 260533.5 47 267342.5 48 269198.0 49 271483.0 50 270728.0 51 259956.0 52 252224.0 53 246903.0 54 228169.5 55 201629.0 56 175113.5 57 161726.0 58 153039.5 59 131283.5 60 107322.5 61 96431.0 62 85004.0 63 62262.0 64 44153.0 65 37359.0 66 32934.0 67 25665.5 68 19225.5 69 13463.5 70 11298.0 71 9417.5 72 6242.0 73 4462.5 74 3978.0 75 3364.5 76 2232.5 77 1371.5 78 1029.0 79 828.5 80 525.5 81 352.5 82 262.0 83 242.0 84 205.0 85 150.0 86 123.0 87 114.0 88 111.0 89 92.5 90 78.5 91 80.0 92 74.0 93 73.5 94 70.0 95 56.0 96 51.0 97 48.5 98 46.0 99 102.5 100 159.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0032492442312680616 2 0.007922314586350216 3 0.011427117352661834 4 0.01865577305817954 5 0.013654127443755673 6 0.013088247830444943 7 0.017049405123620052 8 0.018564502152806847 9 0.017359726201887225 10-11 0.020736749700677067 12-13 0.02007959918199364 14-15 0.008123110578170153 16-17 0.006261184108567107 18-19 0.012285063863165198 20-21 0.011144177546006469 22-23 0.015406528826911481 24-25 0.022699074166190082 26-27 0.013626746172143864 28-29 0.0077762811377539 30-31 0.007821916590440249 32-33 0.009099709265658026 34-35 0.010760839743441135 36-37 0.009099709265658026 38-39 0.01611844188881853 40-41 0.012084267871345263 42-43 0.01549779973228418 44-45 0.018591883424418653 46-47 0.01668432150212926 48-49 0.013991829793634657 50-51 0.02707095053354233 52-53 0.03215473996280163 54-55 0.02445147554934589 56-57 0.014758505398765323 58-59 0.016355746242787544 60-61 0.019331177757937513 62-63 0.025309422059849252 64-65 0.020426428622409894 66-67 0.01993356573339732 68-69 0.029672171336664235 70-71 0.05666097805537113 72-73 0.06792380777836211 74-75 0.07956084821338115 76-77 0.07680446687112567 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 5478197.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.19364976936871 #Duplication Level Percentage of deduplicated Percentage of total 1 70.20462545117532 31.025986293789504 2 13.584463567223226 12.006940503892245 3 5.840559199333984 7.7434688313789195 4 3.048685133353231 5.389300921619753 5 1.8684350064900803 4.128648114682538 6 1.1740254200136004 3.113068093945023 7 0.8057903795732821 2.4927572475671815 8 0.6021519804463318 2.1289034985421553 9 0.4457791386249542 1.7730546414193877 >10 2.1941840794550314 17.07647978946297 >50 0.14233575665880946 4.341782016260966 >100 0.08431042914153819 6.857038884787073 >500 0.0037817504066962344 1.0944944811565152 >1k 8.311505752121195E-4 0.5884215045808392 >5k 0.0 0.0 >10k+ 4.1557528759828506E-5 0.23965517691493596 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13049 0.23819880884166816 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.476254322361901E-5 0.0 2 0.0 0.0 0.0 2.7381271611809507E-4 0.0 3 0.0 0.0 0.0 4.0159198363987276E-4 0.0 4 0.0 0.0 0.0 0.0011500134076959992 0.0 5 0.0 0.0 0.0 0.0023547893586156176 1.8254181074539672E-5 6 0.0 0.0 0.0 0.004691324536156696 1.8254181074539672E-5 7 0.0 0.0 0.0 0.009985037047773199 1.8254181074539672E-5 8 0.0 0.0 0.0 0.016246221156340307 1.8254181074539672E-5 9 0.0 0.0 0.0 0.02265343871350373 3.6508362149079344E-5 10 0.0 0.0 0.0 0.030320194764810394 3.6508362149079344E-5 11 0.0 0.0 0.0 0.03928299767240937 3.6508362149079344E-5 12 0.0 0.0 0.0 0.05122123209515832 3.6508362149079344E-5 13 0.0 0.0 0.0 0.06854444993489646 3.6508362149079344E-5 14 0.0 0.0 0.0 0.08906214946267905 3.6508362149079344E-5 15 0.0 0.0 0.0 0.11266480559205885 3.6508362149079344E-5 16 0.0 0.0 0.0 0.13603015736746962 3.6508362149079344E-5 17 0.0 0.0 0.0 0.15757009103542644 3.6508362149079344E-5 18 0.0 0.0 0.0 0.17998622539496115 3.6508362149079344E-5 19 0.0 0.0 0.0 0.20497619928600597 3.6508362149079344E-5 20 0.0 0.0 0.0 0.23308763814079705 3.6508362149079344E-5 21 0.0 0.0 0.0 0.2633895787245329 3.6508362149079344E-5 22 0.0 0.0 0.0 0.2960098003047353 3.6508362149079344E-5 23 0.0 0.0 0.0 0.33085703197603156 3.6508362149079344E-5 24 0.0 0.0 0.0 0.3652114007583152 5.476254322361901E-5 25 0.0 0.0 0.0 0.4029975555826123 5.476254322361901E-5 26 0.0 0.0 0.0 0.44280992450618334 5.476254322361901E-5 27 0.0 0.0 0.0 0.4825857850676053 5.476254322361901E-5 28 0.0 0.0 0.0 0.5217044951103438 5.476254322361901E-5 29 0.0 0.0 0.0 0.5613708305853184 1.0952508644723802E-4 30 0.0 0.0 0.0 0.6043776811969339 1.0952508644723802E-4 31 0.0 0.0 0.0 0.6517472810853644 1.277792675217777E-4 32 0.0 0.0 0.0 0.7022931084807648 1.277792675217777E-4 33 0.0 0.0 0.0 0.7575485145933963 1.277792675217777E-4 34 0.0 0.0 0.0 0.8134975795868604 1.277792675217777E-4 35 0.0 0.0 0.0 0.8771499089937802 1.277792675217777E-4 36 0.0 0.0 0.0 0.9427919441378249 1.277792675217777E-4 37 0.0 0.0 0.0 1.0077220662199624 1.277792675217777E-4 38 0.0 0.0 0.0 1.0753355529200574 1.277792675217777E-4 39 0.0 0.0 0.0 1.1432411065173451 1.277792675217777E-4 40 0.0 0.0 0.0 1.216130051547982 1.277792675217777E-4 41 0.0 1.8254181074539672E-5 0.0 1.2942579465470117 1.277792675217777E-4 42 0.0 1.8254181074539672E-5 0.0 1.3786105172924596 1.277792675217777E-4 43 0.0 1.8254181074539672E-5 0.0 1.4721084327562517 1.4603344859631738E-4 44 0.0 1.8254181074539672E-5 0.0 1.5679063750354358 1.4603344859631738E-4 45 0.0 1.8254181074539672E-5 0.0 1.6709329730201379 1.4603344859631738E-4 46 0.0 1.8254181074539672E-5 0.0 1.7810787016239102 1.4603344859631738E-4 47 0.0 1.8254181074539672E-5 0.0 1.8953681293316031 1.6428762967085703E-4 48 0.0 1.8254181074539672E-5 0.0 2.011318687517079 1.6428762967085703E-4 49 0.0 1.8254181074539672E-5 0.0 2.1302629313987795 1.6428762967085703E-4 50 0.0 1.8254181074539672E-5 0.0 2.249681783988418 1.6428762967085703E-4 51 0.0 1.8254181074539672E-5 0.0 2.3775888307777175 1.6428762967085703E-4 52 0.0 1.8254181074539672E-5 0.0 2.5118665867620313 1.6428762967085703E-4 53 0.0 1.8254181074539672E-5 0.0 2.6587762360499267 1.6428762967085703E-4 54 0.0 1.8254181074539672E-5 0.0 2.820106688386708 1.6428762967085703E-4 55 0.0 1.8254181074539672E-5 0.0 2.9880816626346225 1.6428762967085703E-4 56 0.0 1.8254181074539672E-5 0.0 3.1664067575518002 1.6428762967085703E-4 57 0.0 1.8254181074539672E-5 0.0 3.3452794779012147 2.0079599181993638E-4 58 0.0 1.8254181074539672E-5 0.0 3.5325126131827678 2.0079599181993638E-4 59 0.0 1.8254181074539672E-5 0.0 3.7211513204070608 2.0079599181993638E-4 60 0.0 1.8254181074539672E-5 0.0 3.911597191557733 2.0079599181993638E-4 61 0.0 1.8254181074539672E-5 0.0 4.106752641425637 2.0079599181993638E-4 62 0.0 1.8254181074539672E-5 0.0 4.31738763684475 2.0079599181993638E-4 63 0.0 1.8254181074539672E-5 0.0 4.53943149543545 2.0079599181993638E-4 64 0.0 1.8254181074539672E-5 0.0 4.770401648571601 2.1905017289447604E-4 65 0.0 1.8254181074539672E-5 0.0 5.0160846716538305 2.1905017289447604E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCGCG 695 0.0 30.134796 56 CGCGGAA 755 0.0 25.392841 59 CGATCGT 880 0.0 24.603487 1 TCGCGCT 830 0.0 24.376429 37 AATTCGC 890 0.0 23.931297 55 CAATTCG 905 0.0 23.142612 54 CGCGCTT 910 0.0 23.013987 38 CTCGCGC 930 0.0 22.135563 36 CGCTCCG 980 0.0 21.00773 25 CGGTTAC 710 0.0 20.996187 4 TCGCGGA 965 0.0 20.970274 58 CCTCGCG 1035 0.0 19.8892 35 CGGCCAA 1045 0.0 19.367018 50 GACGTAT 810 0.0 18.40625 39 AACTCGA 1185 0.0 18.27228 13 ATCGTTC 1245 0.0 17.675505 3 ACTCGAG 1230 0.0 17.60362 14 GATCGTT 1290 0.0 17.058918 2 TTCGCGG 1330 0.0 15.749 57 ACGATCG 665 0.0 15.4824915 59 >>END_MODULE