FastQCFastQC Report
Thu 26 May 2016
SRR936086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5478197
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130620.23843611319563718No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94160.17188136899786555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64570.11786724719830266No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA24000.043.647846100-101
CGTACAT34600.040.511303100-101
GGTATCA156100.037.854241
CGGTATC20250.035.564915100-101
TCGTATC10100.031.822462100-101
ACGTATC8550.031.326206100-101
CGAGTAC25000.031.188372100-101
GTATCAA296650.030.129631
TACGCAG21000.029.193039100-101
CCGTACA19050.027.807144100-101
TCGAGTA10300.027.448448100-101
CCGGTAT7650.026.842258100-101
GGTACAT49500.026.092514100-101
CCGTATC11700.025.944525100-101
GACGCAG18950.025.91231100-101
TCGTACA19100.025.864622100-101
TATACGT8900.025.74391796-97
GCGTACA14400.025.626577100-101
CCTGTCG18650.025.52789796-97
AACGTAT5650.025.28066498-99
Produced by FastQC (version 0.11.5)