FastQCFastQC Report
Thu 26 May 2016
SRR936085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6939093
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTC1400.003418282115.21416656
CGAACTC21650.013.77197625
TAAGGCG24150.013.670011517
TCGAACT22500.013.56824
CGCGCGT2455.928363E-513.0383638
GCCTCGA23050.012.93689921
CTCGAAC23650.012.90889723
TAGCGCG2751.2207669E-512.9062925
GTACGAC6950.011.7481119
CCGTATC5155.2023097E-1011.72638569
CCGGGGT18700.011.3920148
CCTCGAA27650.011.298202522
TTAGTCG3802.3659304E-611.20971215
TAATTCG5803.2196112E-1011.01564635
GTTCGCG5207.623385E-910.92236124
GGCGGAA30250.010.91412220
GTAGACC41750.010.8817868
GACGCGA15750.010.82637769
CGGGGTG19200.010.54057649
AATCCGC20900.010.52889256
Produced by FastQC (version 0.11.5)